Thermodynamic investigation of Z33-antibody interaction leads to selective purification of human antibodies

Mark B. van Eldijk, Ferdinanda C M Smits, Jens C. Thies, Jasmin Mecinović, Jan C M Van Hest*

*Kontaktforfatter for dette arbejde

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

Antibodies, such as IgGs, are widely applied as detection probes, purification ligands and targeting moieties in research and medicine. Protein A from Staphylococcus aureus is capable of selectively binding to antibodies. Z33, a 33 amino acid peptide sequence derived from Protein A, is a minimized binding domain with comparable interaction potential. This peptide was fused to two different proteins without perturbing the properties of both the protein and the Z33-domain. The thermodynamic parameters for the interaction of the fusion proteins with antibodies from various species were determined by isothermal titration calorimetry. This showed that binding was enthalpically driven and entropically unfavorable. A difference in Z33 binding affinity of several orders of magnitude was observed between human and bovine antibodies. This selectivity toward human IgGs was utilized for the efficient and selective purification of human IgGs from mixtures containing bovine IgGs and other proteins by affinity precipitation employing a fusion protein of Z33 and a stimulus-responsive elastin-like polypeptide.

OriginalsprogEngelsk
TidsskriftJournal of Biotechnology
Vol/bind179
Udgave nummer1
Sider (fra-til)32-41
Antal sider10
ISSN0168-1656
DOI
StatusUdgivet - 10. jun. 2014
Udgivet eksterntJa

Fingeraftryk

Antibodies
Purification
Thermodynamics
Proteins
Peptides
Staphylococcal Protein A
Fusion reactions
Elastin
Calorimetry
Titration
Medicine
Ligands
Polypeptides
Amino Acids
Amino acids
Research

Citer dette

van Eldijk, Mark B. ; Smits, Ferdinanda C M ; Thies, Jens C. ; Mecinović, Jasmin ; Van Hest, Jan C M. / Thermodynamic investigation of Z33-antibody interaction leads to selective purification of human antibodies. I: Journal of Biotechnology. 2014 ; Bind 179, Nr. 1. s. 32-41.
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title = "Thermodynamic investigation of Z33-antibody interaction leads to selective purification of human antibodies",
abstract = "Antibodies, such as IgGs, are widely applied as detection probes, purification ligands and targeting moieties in research and medicine. Protein A from Staphylococcus aureus is capable of selectively binding to antibodies. Z33, a 33 amino acid peptide sequence derived from Protein A, is a minimized binding domain with comparable interaction potential. This peptide was fused to two different proteins without perturbing the properties of both the protein and the Z33-domain. The thermodynamic parameters for the interaction of the fusion proteins with antibodies from various species were determined by isothermal titration calorimetry. This showed that binding was enthalpically driven and entropically unfavorable. A difference in Z33 binding affinity of several orders of magnitude was observed between human and bovine antibodies. This selectivity toward human IgGs was utilized for the efficient and selective purification of human IgGs from mixtures containing bovine IgGs and other proteins by affinity precipitation employing a fusion protein of Z33 and a stimulus-responsive elastin-like polypeptide.",
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Thermodynamic investigation of Z33-antibody interaction leads to selective purification of human antibodies. / van Eldijk, Mark B.; Smits, Ferdinanda C M; Thies, Jens C.; Mecinović, Jasmin; Van Hest, Jan C M.

I: Journal of Biotechnology, Bind 179, Nr. 1, 10.06.2014, s. 32-41.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Thermodynamic investigation of Z33-antibody interaction leads to selective purification of human antibodies

AU - van Eldijk, Mark B.

AU - Smits, Ferdinanda C M

AU - Thies, Jens C.

AU - Mecinović, Jasmin

AU - Van Hest, Jan C M

PY - 2014/6/10

Y1 - 2014/6/10

N2 - Antibodies, such as IgGs, are widely applied as detection probes, purification ligands and targeting moieties in research and medicine. Protein A from Staphylococcus aureus is capable of selectively binding to antibodies. Z33, a 33 amino acid peptide sequence derived from Protein A, is a minimized binding domain with comparable interaction potential. This peptide was fused to two different proteins without perturbing the properties of both the protein and the Z33-domain. The thermodynamic parameters for the interaction of the fusion proteins with antibodies from various species were determined by isothermal titration calorimetry. This showed that binding was enthalpically driven and entropically unfavorable. A difference in Z33 binding affinity of several orders of magnitude was observed between human and bovine antibodies. This selectivity toward human IgGs was utilized for the efficient and selective purification of human IgGs from mixtures containing bovine IgGs and other proteins by affinity precipitation employing a fusion protein of Z33 and a stimulus-responsive elastin-like polypeptide.

AB - Antibodies, such as IgGs, are widely applied as detection probes, purification ligands and targeting moieties in research and medicine. Protein A from Staphylococcus aureus is capable of selectively binding to antibodies. Z33, a 33 amino acid peptide sequence derived from Protein A, is a minimized binding domain with comparable interaction potential. This peptide was fused to two different proteins without perturbing the properties of both the protein and the Z33-domain. The thermodynamic parameters for the interaction of the fusion proteins with antibodies from various species were determined by isothermal titration calorimetry. This showed that binding was enthalpically driven and entropically unfavorable. A difference in Z33 binding affinity of several orders of magnitude was observed between human and bovine antibodies. This selectivity toward human IgGs was utilized for the efficient and selective purification of human IgGs from mixtures containing bovine IgGs and other proteins by affinity precipitation employing a fusion protein of Z33 and a stimulus-responsive elastin-like polypeptide.

KW - Antibody purification

KW - Elastin-like polypeptides

KW - Isothermal titration calorimetry

KW - Protein A

KW - Protein engineering

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DO - 10.1016/j.jbiotec.2014.03.023

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VL - 179

SP - 32

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