Thermodynamic, counterion and hydration effects for the incorporation of locked nucleic acid (LNA) nucleotides in duplex

Herleen Kaur, Amit Arora, Jesper Wengel, Souvik Maiti

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Abstrakt

A Locked Nucleic Acid (LNA) monomer is a conformationally restricted nucleotide analogue with an extra 2′-O, 4′-C-methylene bridge added to the ribose ring that is known to exhibit enhanced hybridization affinity towards complementary DNA and RNA, however the underlying thermodynamic basis for this observation are poorly understood. We have evaluated the influence of LNA residues on hybridization thermodynamics, counterions and hydration of DNA•DNA and DNA•RNA heteroduplex using spectroscopic and calorimetric techniques. Thermodynamic analysis for duplex formation using UV and differential scanning calorimetry suggested that LNA-induced stabilization results from a large, favorable increase in the enthalpy of hybridization that compensates for the unfavorable entropy change. The heat capacity change, ΔCp, accompanying the duplex formation, obtained through DSC, has also been reported and has been used to furnish thermodynamic parameters at 37 °C. Furthermore, it was observed that relative to the formation of unmodified duplex, the formation of LNA-modified duplexes was accompanied by a higher uptake of counterions and a lower uptake of water molecules.

OriginalsprogEngelsk
BogserieNucleic Acids Symposium Series
Vol/bind52
Udgave nummer1
Sider (fra-til)425-426
ISSN0261-3166
DOI
StatusUdgivet - 2008
BegivenhedThermodynamic, counterion and hydration effects for the incorporation of locked nucleic acid (LNA) nucleotides in duplex -
Varighed: 24. aug. 2010 → …

Konference

KonferenceThermodynamic, counterion and hydration effects for the incorporation of locked nucleic acid (LNA) nucleotides in duplex
Periode24/08/2010 → …

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Volumne: 52

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