The use of subcutaneous fat tissue for amyloid typing by enzyme-linked immunosorbent assay

K E Olsen, K Sletten, Per Westermark

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

The amyloidoses are biochemically heterogeneous diseases with pathophysiologic deposits of various proteins. The clinical course, prognosis, and therapy are different for each type of amyloidosis and, therefore, a type-specific diagnosis is demanded as early as possible. We describe a method for typing the most common systemic amyloidoses of AL, AA, and transthyretin types by enzyme-linked immunosorbent assay (ELISA), using abdominal wall subcutaneous fat biopsy specimens. The method was tested on 21 abdominal fat biopsy specimens that were sent to the laboratory. Of these, 15 contained amyloid that was successfully characterized in 14 cases. One specimen contained amyloid that did not react with any antisera used. The 6 specimens without amyloid gave no reaction in ELISA. The described ELISA method is reliable and easy to perform, and the tissue sample needed is obtained by minor surgery.

OriginalsprogEngelsk
TidsskriftAmerican Journal of Clinical Pathology
Vol/bind111
Udgave nummer3
Sider (fra-til)355-62
Antal sider8
ISSN0002-9173
StatusUdgivet - mar. 1999

Fingeraftryk

Subcutaneous Fat
Subcutaneous Tissue
Abdominal Subcutaneous Fat
Abdominal Fat
Prealbumin
Abdominal Wall
Proteins

Citer dette

Olsen, K E ; Sletten, K ; Westermark, Per. / The use of subcutaneous fat tissue for amyloid typing by enzyme-linked immunosorbent assay. I: American Journal of Clinical Pathology. 1999 ; Bind 111, Nr. 3. s. 355-62.
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The use of subcutaneous fat tissue for amyloid typing by enzyme-linked immunosorbent assay. / Olsen, K E; Sletten, K; Westermark, Per.

I: American Journal of Clinical Pathology, Bind 111, Nr. 3, 03.1999, s. 355-62.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - The use of subcutaneous fat tissue for amyloid typing by enzyme-linked immunosorbent assay

AU - Olsen, K E

AU - Sletten, K

AU - Westermark, Per

PY - 1999/3

Y1 - 1999/3

N2 - The amyloidoses are biochemically heterogeneous diseases with pathophysiologic deposits of various proteins. The clinical course, prognosis, and therapy are different for each type of amyloidosis and, therefore, a type-specific diagnosis is demanded as early as possible. We describe a method for typing the most common systemic amyloidoses of AL, AA, and transthyretin types by enzyme-linked immunosorbent assay (ELISA), using abdominal wall subcutaneous fat biopsy specimens. The method was tested on 21 abdominal fat biopsy specimens that were sent to the laboratory. Of these, 15 contained amyloid that was successfully characterized in 14 cases. One specimen contained amyloid that did not react with any antisera used. The 6 specimens without amyloid gave no reaction in ELISA. The described ELISA method is reliable and easy to perform, and the tissue sample needed is obtained by minor surgery.

AB - The amyloidoses are biochemically heterogeneous diseases with pathophysiologic deposits of various proteins. The clinical course, prognosis, and therapy are different for each type of amyloidosis and, therefore, a type-specific diagnosis is demanded as early as possible. We describe a method for typing the most common systemic amyloidoses of AL, AA, and transthyretin types by enzyme-linked immunosorbent assay (ELISA), using abdominal wall subcutaneous fat biopsy specimens. The method was tested on 21 abdominal fat biopsy specimens that were sent to the laboratory. Of these, 15 contained amyloid that was successfully characterized in 14 cases. One specimen contained amyloid that did not react with any antisera used. The 6 specimens without amyloid gave no reaction in ELISA. The described ELISA method is reliable and easy to perform, and the tissue sample needed is obtained by minor surgery.

KW - Abdomen

KW - Adipose Tissue

KW - Amino Acid Sequence

KW - Amyloid

KW - Amyloidosis

KW - Animals

KW - Biopsy

KW - Cross Reactions

KW - Enzyme-Linked Immunosorbent Assay

KW - Humans

KW - Immunoglobulin Light Chains

KW - Molecular Sequence Data

KW - Prealbumin

KW - Rabbits

KW - Reproducibility of Results

KW - Serum Amyloid A Protein

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

M3 - Journal article

VL - 111

SP - 355

EP - 362

JO - American Journal of Clinical Pathology

JF - American Journal of Clinical Pathology

SN - 0002-9173

IS - 3

ER -