The Ste locus, a component of the parasitic cry-Ste system of Drosophila melanogaster, encodes a protein that forms crystals in primary spermatocytes and mimics properties of the beta subunit of casein kinase 2

M P Bozzetti, S Massari, P Finelli, F Meggio, L A Pinna, B Boldyreff, O G Issinger, G Palumbo, C Ciriaco, S Bonaccorsi

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

Udgivelsesdato: 1995-Jun-20
OriginalsprogEngelsk
TidsskriftProceedings of the National Academy of Science of the United States of America
Vol/bind92
Udgave nummer13
Sider (fra-til)6067-6071
Antal sider4
ISSN0027-8424
StatusUdgivet - 20. jun. 1995

Fingeraftryk

Casein Kinase II
Drosophila melanogaster
Proteins
Catalytic Domain
Enzymes

Citer dette

@article{ba89ca205a4111de839d000ea68e967b,
title = "The Ste locus, a component of the parasitic cry-Ste system of Drosophila melanogaster, encodes a protein that forms crystals in primary spermatocytes and mimics properties of the beta subunit of casein kinase 2",
abstract = "Males of Drosophila melanogaster lacking the Y chromosome-linked crystal locus show multiple meiotic alterations including chromosome disorganization and prominent crystal formation in primary spermatocytes. These alterations are due to the derepression of the X chromosome-linked Stellate sequences. To understand how the derepression of the Stellate elements gives rise to these abnormalities, we have expressed the protein encoded by the Stellate sequences in bacteria and produced an antibody against the fusion protein. Immunostaining of crystal- testes has clearly shown that the Stellate protein is a major component of the crystals. Moreover, in vitro experiments have shown that this protein can interact with the catalytic alpha subunit of casein kinase 2 enzyme, altering its activity.",
keywords = "Amino Acid Sequence, Animals, Base Sequence, Casein Kinase II, DNA Primers, Drosophila Proteins, Drosophila melanogaster, Genes, Insect, Insect Proteins, Macromolecular Substances, Male, Molecular Sequence Data, Protein Kinases, Protein-Serine-Threonine Kinases, Proteins, Sequence Homology, Amino Acid, Spermatocytes, X Chromosome",
author = "Bozzetti, {M P} and S Massari and P Finelli and F Meggio and Pinna, {L A} and B Boldyreff and Issinger, {O G} and G Palumbo and C Ciriaco and S Bonaccorsi",
year = "1995",
month = "6",
day = "20",
language = "English",
volume = "92",
pages = "6067--6071",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
publisher = "The National Academy of Sciences of the United States of America",
number = "13",

}

The Ste locus, a component of the parasitic cry-Ste system of Drosophila melanogaster, encodes a protein that forms crystals in primary spermatocytes and mimics properties of the beta subunit of casein kinase 2. / Bozzetti, M P; Massari, S; Finelli, P; Meggio, F; Pinna, L A; Boldyreff, B; Issinger, O G; Palumbo, G; Ciriaco, C; Bonaccorsi, S.

I: Proceedings of the National Academy of Science of the United States of America, Bind 92, Nr. 13, 20.06.1995, s. 6067-6071.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - The Ste locus, a component of the parasitic cry-Ste system of Drosophila melanogaster, encodes a protein that forms crystals in primary spermatocytes and mimics properties of the beta subunit of casein kinase 2

AU - Bozzetti, M P

AU - Massari, S

AU - Finelli, P

AU - Meggio, F

AU - Pinna, L A

AU - Boldyreff, B

AU - Issinger, O G

AU - Palumbo, G

AU - Ciriaco, C

AU - Bonaccorsi, S

PY - 1995/6/20

Y1 - 1995/6/20

N2 - Males of Drosophila melanogaster lacking the Y chromosome-linked crystal locus show multiple meiotic alterations including chromosome disorganization and prominent crystal formation in primary spermatocytes. These alterations are due to the derepression of the X chromosome-linked Stellate sequences. To understand how the derepression of the Stellate elements gives rise to these abnormalities, we have expressed the protein encoded by the Stellate sequences in bacteria and produced an antibody against the fusion protein. Immunostaining of crystal- testes has clearly shown that the Stellate protein is a major component of the crystals. Moreover, in vitro experiments have shown that this protein can interact with the catalytic alpha subunit of casein kinase 2 enzyme, altering its activity.

AB - Males of Drosophila melanogaster lacking the Y chromosome-linked crystal locus show multiple meiotic alterations including chromosome disorganization and prominent crystal formation in primary spermatocytes. These alterations are due to the derepression of the X chromosome-linked Stellate sequences. To understand how the derepression of the Stellate elements gives rise to these abnormalities, we have expressed the protein encoded by the Stellate sequences in bacteria and produced an antibody against the fusion protein. Immunostaining of crystal- testes has clearly shown that the Stellate protein is a major component of the crystals. Moreover, in vitro experiments have shown that this protein can interact with the catalytic alpha subunit of casein kinase 2 enzyme, altering its activity.

KW - Amino Acid Sequence

KW - Animals

KW - Base Sequence

KW - Casein Kinase II

KW - DNA Primers

KW - Drosophila Proteins

KW - Drosophila melanogaster

KW - Genes, Insect

KW - Insect Proteins

KW - Macromolecular Substances

KW - Male

KW - Molecular Sequence Data

KW - Protein Kinases

KW - Protein-Serine-Threonine Kinases

KW - Proteins

KW - Sequence Homology, Amino Acid

KW - Spermatocytes

KW - X Chromosome

M3 - Journal article

VL - 92

SP - 6067

EP - 6071

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 13

ER -