The Legs Have it: In Situ Expression of Ion Transporters V-Type H+-ATPase and Na+/K+-ATPase in the Osmoregulatory Leg Organs of the Invading Copepod Eurytemora affinis

Lucie Gerber, Carol Eunmi Lee, Evelyse Grousset, Eva Blondeau-Bidet, Nesrine Boudour Boucheker, Catherine Lorin-Nebel, Mireille Charmantier-Daures, Guy Charmantier

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

The copepod Eurytemora affinis has an unusually broad salinity range, as some populations have recently invaded freshwater habitats independently from their ancestral saline habitats. Prior studies have shown evolutionary shifts in ion transporter activity during freshwater invasions and localization of ion transporters in newly discovered "Crusalis organs" in the swimming legs. The goals of this study were to localize and quantify expression of ion transport enzymes V-type H+-ATPase (VHA) and Na+/K+-ATPase (NKA) in the swimming legs of E. affinis and determine the degree of involvement of each leg in ionic regulation. We confirmed the presence of two distinct types of ionocytes in the Crusalis organs. Both cell types expressed VHA and NKA, and in the freshwater population the location of VHA and NKA in ionocytes was, respectively, apical and basal. Quantification of in situ expression of NKA and VHA established the predominance of swimming leg pairs 3 and 4 in ion transport in both saline and freshwater populations. Increases in VHA expression in swimming legs 3 and 4 of the freshwater population (in fresh water) relative to the saline population (at 15 PSU) arose from an increase in the abundance of VHA per cell rather than an increase in the number of ionocytes. This result suggests a simple mechanism for increasing ion uptake in fresh water. In contrast, the decline in NKA expression in the freshwater population arose from a decrease in ionocyte area in legs 4, likely resulting from decreases in number or size of ionocytes containing NKA. Such results provide insights into mechanisms of ionic regulation for this species, with added insights into evolutionary mechanisms underlying physiological adaptation during habitat invasions.

OriginalsprogEngelsk
TidsskriftPhysiological and Biochemical Zoology
Vol/bind89
Udgave nummer3
Sider (fra-til)233-250
Antal sider18
ISSN1522-2152
DOI
StatusUdgivet - 2016
Udgivet eksterntJa

Fingeraftryk

Eurytemora affinis
H-transporting ATP synthase
sodium-potassium-exchanging ATPase
Fresh Water
transporters
Leg
legs
Copepoda
Ions
ions
ion transport
Population
Ecosystem
habitats
vinyl polysiloxane
sodium-translocating ATPase
cells
salinity

Citer dette

Gerber, Lucie ; Lee, Carol Eunmi ; Grousset, Evelyse ; Blondeau-Bidet, Eva ; Boucheker, Nesrine Boudour ; Lorin-Nebel, Catherine ; Charmantier-Daures, Mireille ; Charmantier, Guy. / The Legs Have it: In Situ Expression of Ion Transporters V-Type H+-ATPase and Na+/K+-ATPase in the Osmoregulatory Leg Organs of the Invading Copepod Eurytemora affinis. I: Physiological and Biochemical Zoology. 2016 ; Bind 89, Nr. 3. s. 233-250.
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title = "The Legs Have it: In Situ Expression of Ion Transporters V-Type H+-ATPase and Na+/K+-ATPase in the Osmoregulatory Leg Organs of the Invading Copepod Eurytemora affinis",
abstract = "The copepod Eurytemora affinis has an unusually broad salinity range, as some populations have recently invaded freshwater habitats independently from their ancestral saline habitats. Prior studies have shown evolutionary shifts in ion transporter activity during freshwater invasions and localization of ion transporters in newly discovered {"}Crusalis organs{"} in the swimming legs. The goals of this study were to localize and quantify expression of ion transport enzymes V-type H+-ATPase (VHA) and Na+/K+-ATPase (NKA) in the swimming legs of E. affinis and determine the degree of involvement of each leg in ionic regulation. We confirmed the presence of two distinct types of ionocytes in the Crusalis organs. Both cell types expressed VHA and NKA, and in the freshwater population the location of VHA and NKA in ionocytes was, respectively, apical and basal. Quantification of in situ expression of NKA and VHA established the predominance of swimming leg pairs 3 and 4 in ion transport in both saline and freshwater populations. Increases in VHA expression in swimming legs 3 and 4 of the freshwater population (in fresh water) relative to the saline population (at 15 PSU) arose from an increase in the abundance of VHA per cell rather than an increase in the number of ionocytes. This result suggests a simple mechanism for increasing ion uptake in fresh water. In contrast, the decline in NKA expression in the freshwater population arose from a decrease in ionocyte area in legs 4, likely resulting from decreases in number or size of ionocytes containing NKA. Such results provide insights into mechanisms of ionic regulation for this species, with added insights into evolutionary mechanisms underlying physiological adaptation during habitat invasions.",
author = "Lucie Gerber and Lee, {Carol Eunmi} and Evelyse Grousset and Eva Blondeau-Bidet and Boucheker, {Nesrine Boudour} and Catherine Lorin-Nebel and Mireille Charmantier-Daures and Guy Charmantier",
year = "2016",
doi = "10.1086/686323",
language = "English",
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Gerber, L, Lee, CE, Grousset, E, Blondeau-Bidet, E, Boucheker, NB, Lorin-Nebel, C, Charmantier-Daures, M & Charmantier, G 2016, 'The Legs Have it: In Situ Expression of Ion Transporters V-Type H+-ATPase and Na+/K+-ATPase in the Osmoregulatory Leg Organs of the Invading Copepod Eurytemora affinis', Physiological and Biochemical Zoology, bind 89, nr. 3, s. 233-250. https://doi.org/10.1086/686323

The Legs Have it: In Situ Expression of Ion Transporters V-Type H+-ATPase and Na+/K+-ATPase in the Osmoregulatory Leg Organs of the Invading Copepod Eurytemora affinis. / Gerber, Lucie; Lee, Carol Eunmi; Grousset, Evelyse; Blondeau-Bidet, Eva; Boucheker, Nesrine Boudour; Lorin-Nebel, Catherine; Charmantier-Daures, Mireille; Charmantier, Guy.

I: Physiological and Biochemical Zoology, Bind 89, Nr. 3, 2016, s. 233-250.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - The Legs Have it: In Situ Expression of Ion Transporters V-Type H+-ATPase and Na+/K+-ATPase in the Osmoregulatory Leg Organs of the Invading Copepod Eurytemora affinis

AU - Gerber, Lucie

AU - Lee, Carol Eunmi

AU - Grousset, Evelyse

AU - Blondeau-Bidet, Eva

AU - Boucheker, Nesrine Boudour

AU - Lorin-Nebel, Catherine

AU - Charmantier-Daures, Mireille

AU - Charmantier, Guy

PY - 2016

Y1 - 2016

N2 - The copepod Eurytemora affinis has an unusually broad salinity range, as some populations have recently invaded freshwater habitats independently from their ancestral saline habitats. Prior studies have shown evolutionary shifts in ion transporter activity during freshwater invasions and localization of ion transporters in newly discovered "Crusalis organs" in the swimming legs. The goals of this study were to localize and quantify expression of ion transport enzymes V-type H+-ATPase (VHA) and Na+/K+-ATPase (NKA) in the swimming legs of E. affinis and determine the degree of involvement of each leg in ionic regulation. We confirmed the presence of two distinct types of ionocytes in the Crusalis organs. Both cell types expressed VHA and NKA, and in the freshwater population the location of VHA and NKA in ionocytes was, respectively, apical and basal. Quantification of in situ expression of NKA and VHA established the predominance of swimming leg pairs 3 and 4 in ion transport in both saline and freshwater populations. Increases in VHA expression in swimming legs 3 and 4 of the freshwater population (in fresh water) relative to the saline population (at 15 PSU) arose from an increase in the abundance of VHA per cell rather than an increase in the number of ionocytes. This result suggests a simple mechanism for increasing ion uptake in fresh water. In contrast, the decline in NKA expression in the freshwater population arose from a decrease in ionocyte area in legs 4, likely resulting from decreases in number or size of ionocytes containing NKA. Such results provide insights into mechanisms of ionic regulation for this species, with added insights into evolutionary mechanisms underlying physiological adaptation during habitat invasions.

AB - The copepod Eurytemora affinis has an unusually broad salinity range, as some populations have recently invaded freshwater habitats independently from their ancestral saline habitats. Prior studies have shown evolutionary shifts in ion transporter activity during freshwater invasions and localization of ion transporters in newly discovered "Crusalis organs" in the swimming legs. The goals of this study were to localize and quantify expression of ion transport enzymes V-type H+-ATPase (VHA) and Na+/K+-ATPase (NKA) in the swimming legs of E. affinis and determine the degree of involvement of each leg in ionic regulation. We confirmed the presence of two distinct types of ionocytes in the Crusalis organs. Both cell types expressed VHA and NKA, and in the freshwater population the location of VHA and NKA in ionocytes was, respectively, apical and basal. Quantification of in situ expression of NKA and VHA established the predominance of swimming leg pairs 3 and 4 in ion transport in both saline and freshwater populations. Increases in VHA expression in swimming legs 3 and 4 of the freshwater population (in fresh water) relative to the saline population (at 15 PSU) arose from an increase in the abundance of VHA per cell rather than an increase in the number of ionocytes. This result suggests a simple mechanism for increasing ion uptake in fresh water. In contrast, the decline in NKA expression in the freshwater population arose from a decrease in ionocyte area in legs 4, likely resulting from decreases in number or size of ionocytes containing NKA. Such results provide insights into mechanisms of ionic regulation for this species, with added insights into evolutionary mechanisms underlying physiological adaptation during habitat invasions.

U2 - 10.1086/686323

DO - 10.1086/686323

M3 - Journal article

VL - 89

SP - 233

EP - 250

JO - Physiological and Biochemical Zoology

JF - Physiological and Biochemical Zoology

SN - 1522-2152

IS - 3

ER -