The importance of -460 C/T and +405 G/C single nucleotide polymorphisms to the function of vascular endothelial growth factor A in colorectal cancer

Torben F Hansen, Karen-Lise G Spindler, Karen A Lorentzen, Dorte A Olsen, Rikke F Andersen, Jan Lindebjerg, Ivan Brandslund, Anders Jakobsen

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

Udgivelsesdato: 2009-Nov-11
OriginalsprogEngelsk
TidsskriftJournal of Cancer Research and Clinical Oncology
Vol/bind136
Udgave nummer5
Sider (fra-til)751-758
ISSN0171-5216
DOI
StatusUdgivet - 11. nov. 2009

Fingeraftryk

Vascular Endothelial Growth Factor A
Single Nucleotide Polymorphism
Colorectal Neoplasms
Proteins
Alleles
Polymerase Chain Reaction
Messenger RNA
DNA

Bibliografisk note

Published online: 11 November 2009.

Citer dette

@article{44cd84f0029011dfaefb000ea68e967b,
title = "The importance of -460 C/T and +405 G/C single nucleotide polymorphisms to the function of vascular endothelial growth factor A in colorectal cancer",
abstract = "PURPOSE: The present study investigated the functional influence of the single nucleotide polymorphisms (SNPs) -460 C/T and +405 G/C at vascular endothelial growth factor A (VEGF-A), mRNA and protein levels in colorectal cancer (CRC) and normal colorectal tissue. METHODS: Blood and tissue were collected from 113 patients surgically resected for colorectal cancer. SNPs were analysed from genomic DNA by PCR, the VEGF-A gene expression analysis was performed by RT-PCR and protein analysis by ELISA. RESULTS: The T-allele in the -460 C/T SNP and the C-allele in the +405 G/C SNP were associated with significantly lower VEGF-A protein levels in normal colorectal tissue. There were no differences in protein levels in the malignant tissue according to genotypes. No differences were observed at the gene expression levels either. CONCLUSION: The results indicate that the two SNPs have a functional influence on the VEGF-A protein levels in normal colorectal tissue. The possible clinical implications of the findings need further investigation.",
author = "Hansen, {Torben F} and Spindler, {Karen-Lise G} and Lorentzen, {Karen A} and Olsen, {Dorte A} and Andersen, {Rikke F} and Jan Lindebjerg and Ivan Brandslund and Anders Jakobsen",
note = "Published online: 11 November 2009.",
year = "2009",
month = "11",
day = "11",
doi = "10.1007/s00432-009-0714-1",
language = "English",
volume = "136",
pages = "751--758",
journal = "Journal of Cancer Research and Clinical Oncology",
issn = "0171-5216",
publisher = "Heinemann",
number = "5",

}

The importance of -460 C/T and +405 G/C single nucleotide polymorphisms to the function of vascular endothelial growth factor A in colorectal cancer. / Hansen, Torben F; Spindler, Karen-Lise G; Lorentzen, Karen A; Olsen, Dorte A; Andersen, Rikke F; Lindebjerg, Jan; Brandslund, Ivan; Jakobsen, Anders.

I: Journal of Cancer Research and Clinical Oncology, Bind 136, Nr. 5, 11.11.2009, s. 751-758.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - The importance of -460 C/T and +405 G/C single nucleotide polymorphisms to the function of vascular endothelial growth factor A in colorectal cancer

AU - Hansen, Torben F

AU - Spindler, Karen-Lise G

AU - Lorentzen, Karen A

AU - Olsen, Dorte A

AU - Andersen, Rikke F

AU - Lindebjerg, Jan

AU - Brandslund, Ivan

AU - Jakobsen, Anders

N1 - Published online: 11 November 2009.

PY - 2009/11/11

Y1 - 2009/11/11

N2 - PURPOSE: The present study investigated the functional influence of the single nucleotide polymorphisms (SNPs) -460 C/T and +405 G/C at vascular endothelial growth factor A (VEGF-A), mRNA and protein levels in colorectal cancer (CRC) and normal colorectal tissue. METHODS: Blood and tissue were collected from 113 patients surgically resected for colorectal cancer. SNPs were analysed from genomic DNA by PCR, the VEGF-A gene expression analysis was performed by RT-PCR and protein analysis by ELISA. RESULTS: The T-allele in the -460 C/T SNP and the C-allele in the +405 G/C SNP were associated with significantly lower VEGF-A protein levels in normal colorectal tissue. There were no differences in protein levels in the malignant tissue according to genotypes. No differences were observed at the gene expression levels either. CONCLUSION: The results indicate that the two SNPs have a functional influence on the VEGF-A protein levels in normal colorectal tissue. The possible clinical implications of the findings need further investigation.

AB - PURPOSE: The present study investigated the functional influence of the single nucleotide polymorphisms (SNPs) -460 C/T and +405 G/C at vascular endothelial growth factor A (VEGF-A), mRNA and protein levels in colorectal cancer (CRC) and normal colorectal tissue. METHODS: Blood and tissue were collected from 113 patients surgically resected for colorectal cancer. SNPs were analysed from genomic DNA by PCR, the VEGF-A gene expression analysis was performed by RT-PCR and protein analysis by ELISA. RESULTS: The T-allele in the -460 C/T SNP and the C-allele in the +405 G/C SNP were associated with significantly lower VEGF-A protein levels in normal colorectal tissue. There were no differences in protein levels in the malignant tissue according to genotypes. No differences were observed at the gene expression levels either. CONCLUSION: The results indicate that the two SNPs have a functional influence on the VEGF-A protein levels in normal colorectal tissue. The possible clinical implications of the findings need further investigation.

U2 - 10.1007/s00432-009-0714-1

DO - 10.1007/s00432-009-0714-1

M3 - Journal article

C2 - 19904558

VL - 136

SP - 751

EP - 758

JO - Journal of Cancer Research and Clinical Oncology

JF - Journal of Cancer Research and Clinical Oncology

SN - 0171-5216

IS - 5

ER -