The human cap-binding complex is functionally connected to the nuclear RNA exosome

Peter Refsing Andersen, Michal Domanski, Maiken Søndergaard Kristiansen, Helena Storvall, Evgenia Ntini, Celine Verheggen, Aleks Schein, Jakob Bunkenborg, Ina Poser, Marie Hallais, Rickard Sandberg, Anthony Hyman, John Lacava, Michael P Rout, Jens S. Andersen, Edouard Bertrand, Torben Heick Jensen

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

Nuclear processing and quality control of eukaryotic RNA is mediated by the RNA exosome, which is regulated by accessory factors. However, the mechanism of exosome recruitment to its ribonucleoprotein (RNP) targets remains poorly understood. Here we report a physical link between the human exosome and the cap-binding complex (CBC). The CBC associates with the ARS2 protein to form CBC-ARS2 (CBCA) and then further connects, together with the ZC3H18 protein, to the nuclear exosome targeting (NEXT) complex, thus forming CBC-NEXT (CBCN). RNA immunoprecipitation using CBCN factors as well as the analysis of combinatorial depletion of CBCN and exosome components underscore the functional relevance of CBC-exosome bridging at the level of target RNA. Specifically, CBCA suppresses read-through products of several RNA families by promoting their transcriptional termination. We suggest that the RNP 5' cap links transcription termination to exosomal RNA degradation through CBCN.
OriginalsprogEngelsk
TidsskriftNature Structural and Molecular Biology
Vol/bind20
Udgave nummer12
Sider (fra-til)1367-1376
ISSN1545-9993
DOI
StatusUdgivet - 2013

Fingeraftryk

Exosomes
Nuclear RNA
RNA
Nuclear Proteins

Citer dette

Andersen, P. R., Domanski, M., Kristiansen, M. S., Storvall, H., Ntini, E., Verheggen, C., ... Jensen, T. H. (2013). The human cap-binding complex is functionally connected to the nuclear RNA exosome. Nature Structural and Molecular Biology, 20(12), 1367-1376. https://doi.org/10.1038/nsmb.2703
Andersen, Peter Refsing ; Domanski, Michal ; Kristiansen, Maiken Søndergaard ; Storvall, Helena ; Ntini, Evgenia ; Verheggen, Celine ; Schein, Aleks ; Bunkenborg, Jakob ; Poser, Ina ; Hallais, Marie ; Sandberg, Rickard ; Hyman, Anthony ; Lacava, John ; Rout, Michael P ; Andersen, Jens S. ; Bertrand, Edouard ; Jensen, Torben Heick. / The human cap-binding complex is functionally connected to the nuclear RNA exosome. I: Nature Structural and Molecular Biology. 2013 ; Bind 20, Nr. 12. s. 1367-1376.
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title = "The human cap-binding complex is functionally connected to the nuclear RNA exosome",
abstract = "Nuclear processing and quality control of eukaryotic RNA is mediated by the RNA exosome, which is regulated by accessory factors. However, the mechanism of exosome recruitment to its ribonucleoprotein (RNP) targets remains poorly understood. Here we report a physical link between the human exosome and the cap-binding complex (CBC). The CBC associates with the ARS2 protein to form CBC-ARS2 (CBCA) and then further connects, together with the ZC3H18 protein, to the nuclear exosome targeting (NEXT) complex, thus forming CBC-NEXT (CBCN). RNA immunoprecipitation using CBCN factors as well as the analysis of combinatorial depletion of CBCN and exosome components underscore the functional relevance of CBC-exosome bridging at the level of target RNA. Specifically, CBCA suppresses read-through products of several RNA families by promoting their transcriptional termination. We suggest that the RNP 5' cap links transcription termination to exosomal RNA degradation through CBCN.",
author = "Andersen, {Peter Refsing} and Michal Domanski and Kristiansen, {Maiken S{\o}ndergaard} and Helena Storvall and Evgenia Ntini and Celine Verheggen and Aleks Schein and Jakob Bunkenborg and Ina Poser and Marie Hallais and Rickard Sandberg and Anthony Hyman and John Lacava and Rout, {Michael P} and Andersen, {Jens S.} and Edouard Bertrand and Jensen, {Torben Heick}",
year = "2013",
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Andersen, PR, Domanski, M, Kristiansen, MS, Storvall, H, Ntini, E, Verheggen, C, Schein, A, Bunkenborg, J, Poser, I, Hallais, M, Sandberg, R, Hyman, A, Lacava, J, Rout, MP, Andersen, JS, Bertrand, E & Jensen, TH 2013, 'The human cap-binding complex is functionally connected to the nuclear RNA exosome', Nature Structural and Molecular Biology, bind 20, nr. 12, s. 1367-1376. https://doi.org/10.1038/nsmb.2703

The human cap-binding complex is functionally connected to the nuclear RNA exosome. / Andersen, Peter Refsing; Domanski, Michal; Kristiansen, Maiken Søndergaard; Storvall, Helena; Ntini, Evgenia; Verheggen, Celine; Schein, Aleks; Bunkenborg, Jakob; Poser, Ina; Hallais, Marie; Sandberg, Rickard; Hyman, Anthony; Lacava, John; Rout, Michael P; Andersen, Jens S.; Bertrand, Edouard; Jensen, Torben Heick.

I: Nature Structural and Molecular Biology, Bind 20, Nr. 12, 2013, s. 1367-1376.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - The human cap-binding complex is functionally connected to the nuclear RNA exosome

AU - Andersen, Peter Refsing

AU - Domanski, Michal

AU - Kristiansen, Maiken Søndergaard

AU - Storvall, Helena

AU - Ntini, Evgenia

AU - Verheggen, Celine

AU - Schein, Aleks

AU - Bunkenborg, Jakob

AU - Poser, Ina

AU - Hallais, Marie

AU - Sandberg, Rickard

AU - Hyman, Anthony

AU - Lacava, John

AU - Rout, Michael P

AU - Andersen, Jens S.

AU - Bertrand, Edouard

AU - Jensen, Torben Heick

PY - 2013

Y1 - 2013

N2 - Nuclear processing and quality control of eukaryotic RNA is mediated by the RNA exosome, which is regulated by accessory factors. However, the mechanism of exosome recruitment to its ribonucleoprotein (RNP) targets remains poorly understood. Here we report a physical link between the human exosome and the cap-binding complex (CBC). The CBC associates with the ARS2 protein to form CBC-ARS2 (CBCA) and then further connects, together with the ZC3H18 protein, to the nuclear exosome targeting (NEXT) complex, thus forming CBC-NEXT (CBCN). RNA immunoprecipitation using CBCN factors as well as the analysis of combinatorial depletion of CBCN and exosome components underscore the functional relevance of CBC-exosome bridging at the level of target RNA. Specifically, CBCA suppresses read-through products of several RNA families by promoting their transcriptional termination. We suggest that the RNP 5' cap links transcription termination to exosomal RNA degradation through CBCN.

AB - Nuclear processing and quality control of eukaryotic RNA is mediated by the RNA exosome, which is regulated by accessory factors. However, the mechanism of exosome recruitment to its ribonucleoprotein (RNP) targets remains poorly understood. Here we report a physical link between the human exosome and the cap-binding complex (CBC). The CBC associates with the ARS2 protein to form CBC-ARS2 (CBCA) and then further connects, together with the ZC3H18 protein, to the nuclear exosome targeting (NEXT) complex, thus forming CBC-NEXT (CBCN). RNA immunoprecipitation using CBCN factors as well as the analysis of combinatorial depletion of CBCN and exosome components underscore the functional relevance of CBC-exosome bridging at the level of target RNA. Specifically, CBCA suppresses read-through products of several RNA families by promoting their transcriptional termination. We suggest that the RNP 5' cap links transcription termination to exosomal RNA degradation through CBCN.

U2 - 10.1038/nsmb.2703

DO - 10.1038/nsmb.2703

M3 - Journal article

C2 - 24270879

VL - 20

SP - 1367

EP - 1376

JO - Nature Structural and Molecular Biology

JF - Nature Structural and Molecular Biology

SN - 1545-9993

IS - 12

ER -

Andersen PR, Domanski M, Kristiansen MS, Storvall H, Ntini E, Verheggen C et al. The human cap-binding complex is functionally connected to the nuclear RNA exosome. Nature Structural and Molecular Biology. 2013;20(12):1367-1376. https://doi.org/10.1038/nsmb.2703