The Göttingen minipig as a model for postprandial hyperlipidaemia in man: experimental observations.

A K Olsen, E-M Bladbjerg, P Marckmann, L F Larsen, A K Hansen

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

 
Udgivelsesdato: 2002-Oct
OriginalsprogEngelsk
TidsskriftLaboratory Animals. Journal of the Laboratory Animal Science Association
Vol/bind36
Udgave nummer4
Sider (fra-til)438-444
Antal sider6
ISSN0023-6772
DOI
StatusUdgivet - 1. okt. 2002

Fingeraftryk

hyperlipidemia
Hyperlipidemias
Fats
lipids
triacylglycerols
olive oil
Lipid Metabolism
Cross-Over Studies
phospholipid emulsion soybean oil
lipid metabolism
dose response
Diet
Lipids
Food
blood
dosage
Olive Oil

Citer dette

@article{419ae540eb7a11dc86ef000ea68e967b,
title = "The G{\"o}ttingen minipig as a model for postprandial hyperlipidaemia in man: experimental observations.",
abstract = "Postprandial hyperlipidaemia is believed to be atherogenic. This study aimed to establish a minipig model to investigate determinants of postprandial lipid metabolism. In a randomized cross-over design seven minipigs were subjected to six different feeding regimens: intragastric fat loads of 1, 2, and 4 g fat (Intralipid, 20{\%}) kg(-1) in two fractions 1.5 h apart (1/3 first, 2/3 second), 2 g fat (Intralipid kg(-1) in one fraction, and 2 g olive oil kg(-1) in two fractions, all after pre-feeding with standard diet, and finally 2 g fat (Intralipid kg(-1) in two fractions without pre-feeding. Blood was sampled before and hourly for 7 h after gavaging, and plasma triglycerides were measured. Triglycerides increased significantly in all the feeding regimens (P < 0.001), except when olive oil was used as the fat source. A borderline significant dose-response effect of the Intralipid dose on the triglyceride response was observed. We found no significant differences in triglyceride response whether 2 g fat (Intralipid kg(-1) was given in one or two fractions, with or without pre-feeding. We conclude that postprandial hyperlipidaemia in minipigs can be induced by gavaging an emulgated lipid solution (1-4 g fat/kg, Intralipid, while olive oil is not applicable. There is no need to administer the fat fractionated or to withhold food prior to administration.",
keywords = "Administration, Oral, Animals, Area Under Curve, Dietary Fats, Disease Models, Animal, Dose-Response Relationship, Drug, Fat Emulsions, Intravenous, Hyperlipidemias, Plant Oils, Postprandial Period, Swine, Swine, Miniature, Time Factors, Triglycerides",
author = "Olsen, {A K} and E-M Bladbjerg and P Marckmann and Larsen, {L F} and Hansen, {A K}",
year = "2002",
month = "10",
day = "1",
doi = "10.1258/002367702320389116",
language = "English",
volume = "36",
pages = "438--444",
journal = "Laboratory Animals",
issn = "0023-6772",
publisher = "SAGE Publications",
number = "4",

}

The Göttingen minipig as a model for postprandial hyperlipidaemia in man: experimental observations. / Olsen, A K; Bladbjerg, E-M; Marckmann, P; Larsen, L F; Hansen, A K.

I: Laboratory Animals. Journal of the Laboratory Animal Science Association, Bind 36, Nr. 4, 01.10.2002, s. 438-444.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - The Göttingen minipig as a model for postprandial hyperlipidaemia in man: experimental observations.

AU - Olsen, A K

AU - Bladbjerg, E-M

AU - Marckmann, P

AU - Larsen, L F

AU - Hansen, A K

PY - 2002/10/1

Y1 - 2002/10/1

N2 - Postprandial hyperlipidaemia is believed to be atherogenic. This study aimed to establish a minipig model to investigate determinants of postprandial lipid metabolism. In a randomized cross-over design seven minipigs were subjected to six different feeding regimens: intragastric fat loads of 1, 2, and 4 g fat (Intralipid, 20%) kg(-1) in two fractions 1.5 h apart (1/3 first, 2/3 second), 2 g fat (Intralipid kg(-1) in one fraction, and 2 g olive oil kg(-1) in two fractions, all after pre-feeding with standard diet, and finally 2 g fat (Intralipid kg(-1) in two fractions without pre-feeding. Blood was sampled before and hourly for 7 h after gavaging, and plasma triglycerides were measured. Triglycerides increased significantly in all the feeding regimens (P < 0.001), except when olive oil was used as the fat source. A borderline significant dose-response effect of the Intralipid dose on the triglyceride response was observed. We found no significant differences in triglyceride response whether 2 g fat (Intralipid kg(-1) was given in one or two fractions, with or without pre-feeding. We conclude that postprandial hyperlipidaemia in minipigs can be induced by gavaging an emulgated lipid solution (1-4 g fat/kg, Intralipid, while olive oil is not applicable. There is no need to administer the fat fractionated or to withhold food prior to administration.

AB - Postprandial hyperlipidaemia is believed to be atherogenic. This study aimed to establish a minipig model to investigate determinants of postprandial lipid metabolism. In a randomized cross-over design seven minipigs were subjected to six different feeding regimens: intragastric fat loads of 1, 2, and 4 g fat (Intralipid, 20%) kg(-1) in two fractions 1.5 h apart (1/3 first, 2/3 second), 2 g fat (Intralipid kg(-1) in one fraction, and 2 g olive oil kg(-1) in two fractions, all after pre-feeding with standard diet, and finally 2 g fat (Intralipid kg(-1) in two fractions without pre-feeding. Blood was sampled before and hourly for 7 h after gavaging, and plasma triglycerides were measured. Triglycerides increased significantly in all the feeding regimens (P < 0.001), except when olive oil was used as the fat source. A borderline significant dose-response effect of the Intralipid dose on the triglyceride response was observed. We found no significant differences in triglyceride response whether 2 g fat (Intralipid kg(-1) was given in one or two fractions, with or without pre-feeding. We conclude that postprandial hyperlipidaemia in minipigs can be induced by gavaging an emulgated lipid solution (1-4 g fat/kg, Intralipid, while olive oil is not applicable. There is no need to administer the fat fractionated or to withhold food prior to administration.

KW - Administration, Oral

KW - Animals

KW - Area Under Curve

KW - Dietary Fats

KW - Disease Models, Animal

KW - Dose-Response Relationship, Drug

KW - Fat Emulsions, Intravenous

KW - Hyperlipidemias

KW - Plant Oils

KW - Postprandial Period

KW - Swine

KW - Swine, Miniature

KW - Time Factors

KW - Triglycerides

U2 - 10.1258/002367702320389116

DO - 10.1258/002367702320389116

M3 - Journal article

C2 - 12396288

VL - 36

SP - 438

EP - 444

JO - Laboratory Animals

JF - Laboratory Animals

SN - 0023-6772

IS - 4

ER -