The effects of globotriaosylceramide tail saturation level on bilayer phases

Weria Pezeshkian, Vitaly V Chaban, Ludger Johannes, Julian Shillcock, John H Ipsen, Himanshu Khandelia

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review


Globotriaosylceramide (Gb3) is a glycosphingolipid present in the plasma membrane that is the natural receptor of the bacterial Shiga toxin. The unsaturation level of Gb3 acyl chains has a drastic impact on lipid bilayer properties and phase behaviour, and on many Gb3-related cellular processes. For example: the Shiga toxin B subunit forms tubular invaginations in the presence of Gb3 with an unsaturated acyl chain (U-Gb3), while in the presence of Gb3 with a saturated acyl chain (S-Gb3) such invagination does not occur. We have used all-atom molecular dynamics simulations to investigate the effects of the Gb3 concentration and its acyl chain saturation on the phase behaviour of a mixed bilayer of dioleoylphosphatidylcholine and Gb3. The simulation results show that: (1) the Gb3 acyl chains (longer tails) from one leaflet interdigitate into the opposing leaflet and lead to significant bilayer rigidification and immobilisation of the lipid tails. S-Gb3 can form a highly ordered, relatively immobile phase which is resistant to bending while these changes for U-Gb3 are not significant. (2) At low concentrations of Gb3, U-Gb3 and S-Gb3 have a similar impact on the bilayer reminiscent of the effect of sphingomyelin lipids and (3) At higher Gb3 concentrations, U-Gb3 mixes better with dioleoylphosphatidylcholine than S-Gb3. Our simulations also provide the first molecular level structural model of Gb3 in membranes.

TidsskriftSoft Matter
Udgave nummer7
Sider (fra-til)1352-1361
Antal sider10
StatusUdgivet - 4. feb. 2015


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