The effect of smoking on the urinary excretion of 8-oxodG and 8-oxoGuo in patients with type 2 diabetes

Anne-Sofie Sørensen, Laura Kofoed Kjær, Kasper Meidahl Petersen, Trine Maxel Henriksen, Vanja Cejvanovic, Oluf Pedersen, Torben Hansen, Cramer Kjeldahl Christensen, Ivan Brandslund, Henrik Enghusen Poulsen

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

Over the past decades, attention has been paid to understanding the impact of oxidative stress and related modifications of DNA and RNA on various human health risks. A recent meta-analysis comprising 1915 smokers and 3462 non-smokers found a significantly higher level of DNA oxidation measured as urinary 8-oxo-7, 8-dihydro-2'-deoxyguanosine (8-oxodG) excretion in smokers compared with non-smokers in a healthy population. We aimed to investigate if an increased urinary excretion of 8-oxodG in smokers versus never smokers and former smokers could be verified in a population with type 2 diabetes. Additionally, we measured RNA oxidation levels through urinary excretion of 8-oxo-7, 8-dihydroguanosine (8-oxoGuo). Our study included urinary samples from 2721 type 2 diabetic patients, analyzed using ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS). Logistic regression was used to examine the relationship between daily smokers (n = 462) versus former (n = 1341) and never smokers (n = 918) regarding the RNA and DNA oxidation, respectively. We did not find any significant effect of smoking on urinary excretion of 8-oxodG or 8-oxoGuo in our study. Due to a sparse study area, it is still too early to draw any conclusions on smoking and RNA-oxidation. Regarding DNA oxidation, our study suggests that the effect of smoking seen in healthy populations might be attenuated in patients with type 2 diabetes.

OriginalsprogEngelsk
TidsskriftScandinavian Journal of Clinical & Laboratory Investigation
Vol/bind77
Udgave nummer4
Sider (fra-til)253-258
ISSN0036-5513
DOI
StatusUdgivet - jul. 2017

Fingeraftryk

Type 2 Diabetes Mellitus
Smoking
RNA
DNA
Population
Tandem Mass Spectrometry
Liquid Chromatography
Meta-Analysis
Logistic Models
8-hydroxyguanosine
8-oxo-7-hydrodeoxyguanosine
Health

Citer dette

Sørensen, A-S., Kjær, L. K., Petersen, K. M., Henriksen, T. M., Cejvanovic, V., Pedersen, O., ... Poulsen, H. E. (2017). The effect of smoking on the urinary excretion of 8-oxodG and 8-oxoGuo in patients with type 2 diabetes. Scandinavian Journal of Clinical & Laboratory Investigation, 77(4), 253-258. https://doi.org/10.1080/00365513.2017.1299208
Sørensen, Anne-Sofie ; Kjær, Laura Kofoed ; Petersen, Kasper Meidahl ; Henriksen, Trine Maxel ; Cejvanovic, Vanja ; Pedersen, Oluf ; Hansen, Torben ; Christensen, Cramer Kjeldahl ; Brandslund, Ivan ; Poulsen, Henrik Enghusen. / The effect of smoking on the urinary excretion of 8-oxodG and 8-oxoGuo in patients with type 2 diabetes. I: Scandinavian Journal of Clinical & Laboratory Investigation. 2017 ; Bind 77, Nr. 4. s. 253-258.
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abstract = "Over the past decades, attention has been paid to understanding the impact of oxidative stress and related modifications of DNA and RNA on various human health risks. A recent meta-analysis comprising 1915 smokers and 3462 non-smokers found a significantly higher level of DNA oxidation measured as urinary 8-oxo-7, 8-dihydro-2'-deoxyguanosine (8-oxodG) excretion in smokers compared with non-smokers in a healthy population. We aimed to investigate if an increased urinary excretion of 8-oxodG in smokers versus never smokers and former smokers could be verified in a population with type 2 diabetes. Additionally, we measured RNA oxidation levels through urinary excretion of 8-oxo-7, 8-dihydroguanosine (8-oxoGuo). Our study included urinary samples from 2721 type 2 diabetic patients, analyzed using ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS). Logistic regression was used to examine the relationship between daily smokers (n = 462) versus former (n = 1341) and never smokers (n = 918) regarding the RNA and DNA oxidation, respectively. We did not find any significant effect of smoking on urinary excretion of 8-oxodG or 8-oxoGuo in our study. Due to a sparse study area, it is still too early to draw any conclusions on smoking and RNA-oxidation. Regarding DNA oxidation, our study suggests that the effect of smoking seen in healthy populations might be attenuated in patients with type 2 diabetes.",
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Sørensen, A-S, Kjær, LK, Petersen, KM, Henriksen, TM, Cejvanovic, V, Pedersen, O, Hansen, T, Christensen, CK, Brandslund, I & Poulsen, HE 2017, 'The effect of smoking on the urinary excretion of 8-oxodG and 8-oxoGuo in patients with type 2 diabetes', Scandinavian Journal of Clinical & Laboratory Investigation, bind 77, nr. 4, s. 253-258. https://doi.org/10.1080/00365513.2017.1299208

The effect of smoking on the urinary excretion of 8-oxodG and 8-oxoGuo in patients with type 2 diabetes. / Sørensen, Anne-Sofie; Kjær, Laura Kofoed; Petersen, Kasper Meidahl; Henriksen, Trine Maxel; Cejvanovic, Vanja; Pedersen, Oluf; Hansen, Torben; Christensen, Cramer Kjeldahl; Brandslund, Ivan; Poulsen, Henrik Enghusen.

I: Scandinavian Journal of Clinical & Laboratory Investigation, Bind 77, Nr. 4, 07.2017, s. 253-258.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - The effect of smoking on the urinary excretion of 8-oxodG and 8-oxoGuo in patients with type 2 diabetes

AU - Sørensen, Anne-Sofie

AU - Kjær, Laura Kofoed

AU - Petersen, Kasper Meidahl

AU - Henriksen, Trine Maxel

AU - Cejvanovic, Vanja

AU - Pedersen, Oluf

AU - Hansen, Torben

AU - Christensen, Cramer Kjeldahl

AU - Brandslund, Ivan

AU - Poulsen, Henrik Enghusen

PY - 2017/7

Y1 - 2017/7

N2 - Over the past decades, attention has been paid to understanding the impact of oxidative stress and related modifications of DNA and RNA on various human health risks. A recent meta-analysis comprising 1915 smokers and 3462 non-smokers found a significantly higher level of DNA oxidation measured as urinary 8-oxo-7, 8-dihydro-2'-deoxyguanosine (8-oxodG) excretion in smokers compared with non-smokers in a healthy population. We aimed to investigate if an increased urinary excretion of 8-oxodG in smokers versus never smokers and former smokers could be verified in a population with type 2 diabetes. Additionally, we measured RNA oxidation levels through urinary excretion of 8-oxo-7, 8-dihydroguanosine (8-oxoGuo). Our study included urinary samples from 2721 type 2 diabetic patients, analyzed using ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS). Logistic regression was used to examine the relationship between daily smokers (n = 462) versus former (n = 1341) and never smokers (n = 918) regarding the RNA and DNA oxidation, respectively. We did not find any significant effect of smoking on urinary excretion of 8-oxodG or 8-oxoGuo in our study. Due to a sparse study area, it is still too early to draw any conclusions on smoking and RNA-oxidation. Regarding DNA oxidation, our study suggests that the effect of smoking seen in healthy populations might be attenuated in patients with type 2 diabetes.

AB - Over the past decades, attention has been paid to understanding the impact of oxidative stress and related modifications of DNA and RNA on various human health risks. A recent meta-analysis comprising 1915 smokers and 3462 non-smokers found a significantly higher level of DNA oxidation measured as urinary 8-oxo-7, 8-dihydro-2'-deoxyguanosine (8-oxodG) excretion in smokers compared with non-smokers in a healthy population. We aimed to investigate if an increased urinary excretion of 8-oxodG in smokers versus never smokers and former smokers could be verified in a population with type 2 diabetes. Additionally, we measured RNA oxidation levels through urinary excretion of 8-oxo-7, 8-dihydroguanosine (8-oxoGuo). Our study included urinary samples from 2721 type 2 diabetic patients, analyzed using ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS). Logistic regression was used to examine the relationship between daily smokers (n = 462) versus former (n = 1341) and never smokers (n = 918) regarding the RNA and DNA oxidation, respectively. We did not find any significant effect of smoking on urinary excretion of 8-oxodG or 8-oxoGuo in our study. Due to a sparse study area, it is still too early to draw any conclusions on smoking and RNA-oxidation. Regarding DNA oxidation, our study suggests that the effect of smoking seen in healthy populations might be attenuated in patients with type 2 diabetes.

KW - Journal Article

U2 - 10.1080/00365513.2017.1299208

DO - 10.1080/00365513.2017.1299208

M3 - Journal article

VL - 77

SP - 253

EP - 258

JO - Scandinavian Journal of Clinical & Laboratory Investigation

JF - Scandinavian Journal of Clinical & Laboratory Investigation

SN - 0036-5513

IS - 4

ER -