The distribution of free calcium ions in the cholesteatoma epithelium

Viggo Svane-Knudsen, Gurli Rasmussen, Peter D Ottosen

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

The distribution of free calcium ions in normal skin and cholesteatoma epithelium was investigated using the oxalate precipitation method. In agreement with previous observations, we could demonstrate a calcium ion gradient in normal epidermis where the cells in stratum basale and spinosum reside in an environment containing relatively low concentrations of calcium ions, whereas the outer stratum granulosum contained abundant calcium. The concentration declined precipitously in the stratum corneum. In contrast, in cholesteatomas, the gradient was perturbed in some areas of the nucleated layers and areas appeared where oblong accumulations of free calcium ions were found basally in the stratum. These findings provide evidence that fluctuations in epidermal calcium in cholesteatoma epithelium may underlie the abnormal desquamation, may contribute to the formation of an abnormal permeability barrier and may regulate terminal events in epidermal differentiation.

OriginalsprogEngelsk
TidsskriftEuropean Archives of Oto-Rhino-Laryngology
Vol/bind262
Udgave nummer1
Sider (fra-til)77-80
Antal sider4
ISSN0937-4477
DOI
StatusUdgivet - jan. 2005

Fingeraftryk

Cholesteatoma
Epithelium
Ions
Epidermis
Skin

Citer dette

Svane-Knudsen, Viggo ; Rasmussen, Gurli ; Ottosen, Peter D. / The distribution of free calcium ions in the cholesteatoma epithelium. I: European Archives of Oto-Rhino-Laryngology. 2005 ; Bind 262, Nr. 1. s. 77-80.
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abstract = "The distribution of free calcium ions in normal skin and cholesteatoma epithelium was investigated using the oxalate precipitation method. In agreement with previous observations, we could demonstrate a calcium ion gradient in normal epidermis where the cells in stratum basale and spinosum reside in an environment containing relatively low concentrations of calcium ions, whereas the outer stratum granulosum contained abundant calcium. The concentration declined precipitously in the stratum corneum. In contrast, in cholesteatomas, the gradient was perturbed in some areas of the nucleated layers and areas appeared where oblong accumulations of free calcium ions were found basally in the stratum. These findings provide evidence that fluctuations in epidermal calcium in cholesteatoma epithelium may underlie the abnormal desquamation, may contribute to the formation of an abnormal permeability barrier and may regulate terminal events in epidermal differentiation.",
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The distribution of free calcium ions in the cholesteatoma epithelium. / Svane-Knudsen, Viggo; Rasmussen, Gurli; Ottosen, Peter D.

I: European Archives of Oto-Rhino-Laryngology, Bind 262, Nr. 1, 01.2005, s. 77-80.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - The distribution of free calcium ions in the cholesteatoma epithelium

AU - Svane-Knudsen, Viggo

AU - Rasmussen, Gurli

AU - Ottosen, Peter D

PY - 2005/1

Y1 - 2005/1

N2 - The distribution of free calcium ions in normal skin and cholesteatoma epithelium was investigated using the oxalate precipitation method. In agreement with previous observations, we could demonstrate a calcium ion gradient in normal epidermis where the cells in stratum basale and spinosum reside in an environment containing relatively low concentrations of calcium ions, whereas the outer stratum granulosum contained abundant calcium. The concentration declined precipitously in the stratum corneum. In contrast, in cholesteatomas, the gradient was perturbed in some areas of the nucleated layers and areas appeared where oblong accumulations of free calcium ions were found basally in the stratum. These findings provide evidence that fluctuations in epidermal calcium in cholesteatoma epithelium may underlie the abnormal desquamation, may contribute to the formation of an abnormal permeability barrier and may regulate terminal events in epidermal differentiation.

AB - The distribution of free calcium ions in normal skin and cholesteatoma epithelium was investigated using the oxalate precipitation method. In agreement with previous observations, we could demonstrate a calcium ion gradient in normal epidermis where the cells in stratum basale and spinosum reside in an environment containing relatively low concentrations of calcium ions, whereas the outer stratum granulosum contained abundant calcium. The concentration declined precipitously in the stratum corneum. In contrast, in cholesteatomas, the gradient was perturbed in some areas of the nucleated layers and areas appeared where oblong accumulations of free calcium ions were found basally in the stratum. These findings provide evidence that fluctuations in epidermal calcium in cholesteatoma epithelium may underlie the abnormal desquamation, may contribute to the formation of an abnormal permeability barrier and may regulate terminal events in epidermal differentiation.

KW - Calcium Channels

KW - Cholesteatoma, Middle Ear

KW - Epithelium

KW - Humans

KW - Tympanic Membrane

U2 - 10.1007/s00405-004-0737-9

DO - 10.1007/s00405-004-0737-9

M3 - Journal article

VL - 262

SP - 77

EP - 80

JO - European Archives of Oto-Rhino-Laryngology

JF - European Archives of Oto-Rhino-Laryngology

SN - 0937-4477

IS - 1

ER -