The diffusion dynamics of PEGylated liposomes in the intact vitreous of the ex vivo porcine eye

A fluorescence correlation spectroscopy and biodistribution study

Anne Z. Eriksen, Jonathan Brewer, Thomas L. Andresen, Andrew J. Urquhart*

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Resumé

The diffusion dynamics of nanocarriers in the vitreous and the influence of nanocarrier physicochemical properties on these dynamics is an important aspect of the efficacy of intravitreal administered nanomedicines for the treatment of posterior segment eye diseases. Here we use fluorescence correlation spectroscopy (FCS) to determine liposome diffusion coefficients in the intact vitreous (DVit) of ex vivo porcine eyes using a modified Miyake-Apple technique to minimize the disruption of the vitreous fine structure. We chose to investigate whether the zeta potential of polyethylene glycol functionalized (i.e. PEGylated) liposomes altered liposome in situ diffusion dynamics in the vitreous. Non-PEGylated cationic nanocarriers have previously shown little to no diffusion in the vitreous, whilst neutral and anionic have shown diffusion. The liposomes investigated had diameters below 150 nm and zeta potentials ranging from −20 to +12 mV. We observed that PEGylated cationic liposomes had significantly lower DVit values (1.14 μm2s−1) than PEGylated neutral and anionic liposomes (2.78 and 2.87 μm2 s−1). However, PEGylated cationic liposomes had a similar biodistribution profile across the vitreous to the other systems. These results show that PEGylated cationic liposomes with limited cationic charge can diffuse across the vitreous and indicate that the vitreous as a barrier to nanocarriers (Ø < 500 nm) is more complicated than simply an electrostatic barrier as previously suggested.

OriginalsprogEngelsk
TidsskriftInternational Journal of Pharmaceutics
Vol/bind522
Udgave nummer1-2
Sider (fra-til)90-97
ISSN0378-5173
DOI
StatusUdgivet - 2017

Fingeraftryk

Eye Diseases
Malus
Static Electricity

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abstract = "The diffusion dynamics of nanocarriers in the vitreous and the influence of nanocarrier physicochemical properties on these dynamics is an important aspect of the efficacy of intravitreal administered nanomedicines for the treatment of posterior segment eye diseases. Here we use fluorescence correlation spectroscopy (FCS) to determine liposome diffusion coefficients in the intact vitreous (DVit) of ex vivo porcine eyes using a modified Miyake-Apple technique to minimize the disruption of the vitreous fine structure. We chose to investigate whether the zeta potential of polyethylene glycol functionalized (i.e. PEGylated) liposomes altered liposome in situ diffusion dynamics in the vitreous. Non-PEGylated cationic nanocarriers have previously shown little to no diffusion in the vitreous, whilst neutral and anionic have shown diffusion. The liposomes investigated had diameters below 150 nm and zeta potentials ranging from −20 to +12 mV. We observed that PEGylated cationic liposomes had significantly lower DVit values (1.14 μm2s−1) than PEGylated neutral and anionic liposomes (2.78 and 2.87 μm2 s−1). However, PEGylated cationic liposomes had a similar biodistribution profile across the vitreous to the other systems. These results show that PEGylated cationic liposomes with limited cationic charge can diffuse across the vitreous and indicate that the vitreous as a barrier to nanocarriers ({\O} < 500 nm) is more complicated than simply an electrostatic barrier as previously suggested.",
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author = "Eriksen, {Anne Z.} and Jonathan Brewer and Andresen, {Thomas L.} and Urquhart, {Andrew J.}",
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The diffusion dynamics of PEGylated liposomes in the intact vitreous of the ex vivo porcine eye : A fluorescence correlation spectroscopy and biodistribution study. / Eriksen, Anne Z.; Brewer, Jonathan; Andresen, Thomas L.; Urquhart, Andrew J.

I: International Journal of Pharmaceutics, Bind 522, Nr. 1-2, 2017, s. 90-97.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - The diffusion dynamics of PEGylated liposomes in the intact vitreous of the ex vivo porcine eye

T2 - A fluorescence correlation spectroscopy and biodistribution study

AU - Eriksen, Anne Z.

AU - Brewer, Jonathan

AU - Andresen, Thomas L.

AU - Urquhart, Andrew J.

PY - 2017

Y1 - 2017

N2 - The diffusion dynamics of nanocarriers in the vitreous and the influence of nanocarrier physicochemical properties on these dynamics is an important aspect of the efficacy of intravitreal administered nanomedicines for the treatment of posterior segment eye diseases. Here we use fluorescence correlation spectroscopy (FCS) to determine liposome diffusion coefficients in the intact vitreous (DVit) of ex vivo porcine eyes using a modified Miyake-Apple technique to minimize the disruption of the vitreous fine structure. We chose to investigate whether the zeta potential of polyethylene glycol functionalized (i.e. PEGylated) liposomes altered liposome in situ diffusion dynamics in the vitreous. Non-PEGylated cationic nanocarriers have previously shown little to no diffusion in the vitreous, whilst neutral and anionic have shown diffusion. The liposomes investigated had diameters below 150 nm and zeta potentials ranging from −20 to +12 mV. We observed that PEGylated cationic liposomes had significantly lower DVit values (1.14 μm2s−1) than PEGylated neutral and anionic liposomes (2.78 and 2.87 μm2 s−1). However, PEGylated cationic liposomes had a similar biodistribution profile across the vitreous to the other systems. These results show that PEGylated cationic liposomes with limited cationic charge can diffuse across the vitreous and indicate that the vitreous as a barrier to nanocarriers (Ø < 500 nm) is more complicated than simply an electrostatic barrier as previously suggested.

AB - The diffusion dynamics of nanocarriers in the vitreous and the influence of nanocarrier physicochemical properties on these dynamics is an important aspect of the efficacy of intravitreal administered nanomedicines for the treatment of posterior segment eye diseases. Here we use fluorescence correlation spectroscopy (FCS) to determine liposome diffusion coefficients in the intact vitreous (DVit) of ex vivo porcine eyes using a modified Miyake-Apple technique to minimize the disruption of the vitreous fine structure. We chose to investigate whether the zeta potential of polyethylene glycol functionalized (i.e. PEGylated) liposomes altered liposome in situ diffusion dynamics in the vitreous. Non-PEGylated cationic nanocarriers have previously shown little to no diffusion in the vitreous, whilst neutral and anionic have shown diffusion. The liposomes investigated had diameters below 150 nm and zeta potentials ranging from −20 to +12 mV. We observed that PEGylated cationic liposomes had significantly lower DVit values (1.14 μm2s−1) than PEGylated neutral and anionic liposomes (2.78 and 2.87 μm2 s−1). However, PEGylated cationic liposomes had a similar biodistribution profile across the vitreous to the other systems. These results show that PEGylated cationic liposomes with limited cationic charge can diffuse across the vitreous and indicate that the vitreous as a barrier to nanocarriers (Ø < 500 nm) is more complicated than simply an electrostatic barrier as previously suggested.

KW - Diffusion

KW - Fluorescence

KW - Liposomes

KW - Ocular

KW - Vitreous

U2 - 10.1016/j.ijpharm.2017.03.003

DO - 10.1016/j.ijpharm.2017.03.003

M3 - Journal article

VL - 522

SP - 90

EP - 97

JO - International Journal of Pharmaceutics

JF - International Journal of Pharmaceutics

SN - 0378-5173

IS - 1-2

ER -