The cell-free fetal DNA fraction in maternal blood decreases after physical activity

Jacob Mørup Schlütter, Lotte Hatt, Cathrine Bach, Ida Kirkegaard, Steen Kølvraa, Niels Uldbjerg

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

OBJECTIVE: If noninvasive prenatal testing using next generation sequencing is to be effective for pregnant women, a cell-free fetal DNA (cffDNA) fraction above 4% is essential unless the depth of sequencing is increased. This study's objective is to determine whether physical activity has an effect on the proportion of cell-free DNA (cfDNA) arising from the fetus (fetal fraction).

METHODS: Nine pregnant women carrying male fetuses at gestational age 12(+0)  weeks to 14(+6)  weeks were included. Plasma from nine pregnant women was drawn prior to, immediately after, and 30 min after 30 min of cycling with a pulse-rate of 150 beats per minute. The concentrations of cffDNA (DYS14) and cfDNA (RASSF1A) were assessed using quantitative real-time polymerase chain reaction.

RESULTS: The fetal fraction decreased significantly in all participants after physical activity (p < 0.01), a decrease varying from 1-17 percentage points. This was due to a significant increase in the concentration of cfDNA (p < 0.01), whereas the concentration of cffDNA remained the same. This alteration of the fetal fraction was not present 30 min after physical activity.

CONCLUSION: When planning the timing of noninvasive prenatal diagnosis based on the fetal fraction, physical activity prior to sampling should be avoided.

OriginalsprogEngelsk
TidsskriftPrenatal Diagnosis
Vol/bind34
Udgave nummer4
Sider (fra-til)341-4
Antal sider4
ISSN0197-3851
DOI
StatusUdgivet - apr. 2014

Fingeraftryk

Mothers
Exercise
DNA
Pregnant Women
Fetus
Prenatal Diagnosis
Gestational Age
Real-Time Polymerase Chain Reaction

Citer dette

Schlütter, J. M., Hatt, L., Bach, C., Kirkegaard, I., Kølvraa, S., & Uldbjerg, N. (2014). The cell-free fetal DNA fraction in maternal blood decreases after physical activity. Prenatal Diagnosis, 34(4), 341-4. https://doi.org/10.1002/pd.4306
Schlütter, Jacob Mørup ; Hatt, Lotte ; Bach, Cathrine ; Kirkegaard, Ida ; Kølvraa, Steen ; Uldbjerg, Niels. / The cell-free fetal DNA fraction in maternal blood decreases after physical activity. I: Prenatal Diagnosis. 2014 ; Bind 34, Nr. 4. s. 341-4.
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abstract = "OBJECTIVE: If noninvasive prenatal testing using next generation sequencing is to be effective for pregnant women, a cell-free fetal DNA (cffDNA) fraction above 4{\%} is essential unless the depth of sequencing is increased. This study's objective is to determine whether physical activity has an effect on the proportion of cell-free DNA (cfDNA) arising from the fetus (fetal fraction).METHODS: Nine pregnant women carrying male fetuses at gestational age 12(+0)  weeks to 14(+6)  weeks were included. Plasma from nine pregnant women was drawn prior to, immediately after, and 30 min after 30 min of cycling with a pulse-rate of 150 beats per minute. The concentrations of cffDNA (DYS14) and cfDNA (RASSF1A) were assessed using quantitative real-time polymerase chain reaction.RESULTS: The fetal fraction decreased significantly in all participants after physical activity (p < 0.01), a decrease varying from 1-17 percentage points. This was due to a significant increase in the concentration of cfDNA (p < 0.01), whereas the concentration of cffDNA remained the same. This alteration of the fetal fraction was not present 30 min after physical activity.CONCLUSION: When planning the timing of noninvasive prenatal diagnosis based on the fetal fraction, physical activity prior to sampling should be avoided.",
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Schlütter, JM, Hatt, L, Bach, C, Kirkegaard, I, Kølvraa, S & Uldbjerg, N 2014, 'The cell-free fetal DNA fraction in maternal blood decreases after physical activity', Prenatal Diagnosis, bind 34, nr. 4, s. 341-4. https://doi.org/10.1002/pd.4306

The cell-free fetal DNA fraction in maternal blood decreases after physical activity. / Schlütter, Jacob Mørup; Hatt, Lotte; Bach, Cathrine; Kirkegaard, Ida; Kølvraa, Steen; Uldbjerg, Niels.

I: Prenatal Diagnosis, Bind 34, Nr. 4, 04.2014, s. 341-4.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - The cell-free fetal DNA fraction in maternal blood decreases after physical activity

AU - Schlütter, Jacob Mørup

AU - Hatt, Lotte

AU - Bach, Cathrine

AU - Kirkegaard, Ida

AU - Kølvraa, Steen

AU - Uldbjerg, Niels

N1 - © 2013 John Wiley & Sons, Ltd.

PY - 2014/4

Y1 - 2014/4

N2 - OBJECTIVE: If noninvasive prenatal testing using next generation sequencing is to be effective for pregnant women, a cell-free fetal DNA (cffDNA) fraction above 4% is essential unless the depth of sequencing is increased. This study's objective is to determine whether physical activity has an effect on the proportion of cell-free DNA (cfDNA) arising from the fetus (fetal fraction).METHODS: Nine pregnant women carrying male fetuses at gestational age 12(+0)  weeks to 14(+6)  weeks were included. Plasma from nine pregnant women was drawn prior to, immediately after, and 30 min after 30 min of cycling with a pulse-rate of 150 beats per minute. The concentrations of cffDNA (DYS14) and cfDNA (RASSF1A) were assessed using quantitative real-time polymerase chain reaction.RESULTS: The fetal fraction decreased significantly in all participants after physical activity (p < 0.01), a decrease varying from 1-17 percentage points. This was due to a significant increase in the concentration of cfDNA (p < 0.01), whereas the concentration of cffDNA remained the same. This alteration of the fetal fraction was not present 30 min after physical activity.CONCLUSION: When planning the timing of noninvasive prenatal diagnosis based on the fetal fraction, physical activity prior to sampling should be avoided.

AB - OBJECTIVE: If noninvasive prenatal testing using next generation sequencing is to be effective for pregnant women, a cell-free fetal DNA (cffDNA) fraction above 4% is essential unless the depth of sequencing is increased. This study's objective is to determine whether physical activity has an effect on the proportion of cell-free DNA (cfDNA) arising from the fetus (fetal fraction).METHODS: Nine pregnant women carrying male fetuses at gestational age 12(+0)  weeks to 14(+6)  weeks were included. Plasma from nine pregnant women was drawn prior to, immediately after, and 30 min after 30 min of cycling with a pulse-rate of 150 beats per minute. The concentrations of cffDNA (DYS14) and cfDNA (RASSF1A) were assessed using quantitative real-time polymerase chain reaction.RESULTS: The fetal fraction decreased significantly in all participants after physical activity (p < 0.01), a decrease varying from 1-17 percentage points. This was due to a significant increase in the concentration of cfDNA (p < 0.01), whereas the concentration of cffDNA remained the same. This alteration of the fetal fraction was not present 30 min after physical activity.CONCLUSION: When planning the timing of noninvasive prenatal diagnosis based on the fetal fraction, physical activity prior to sampling should be avoided.

U2 - 10.1002/pd.4306

DO - 10.1002/pd.4306

M3 - Journal article

VL - 34

SP - 341

EP - 344

JO - Prenatal Diagnosis

JF - Prenatal Diagnosis

SN - 0197-3851

IS - 4

ER -

Schlütter JM, Hatt L, Bach C, Kirkegaard I, Kølvraa S, Uldbjerg N. The cell-free fetal DNA fraction in maternal blood decreases after physical activity. Prenatal Diagnosis. 2014 apr;34(4):341-4. https://doi.org/10.1002/pd.4306