The acyl-CoA binding protein is required for normal epidermal barrier function in mice

Maria Bloksgaard, Signe Bek, Ann-Britt Marcher, Ditte Neess, Jonathan Brewer, Hans Kristian Hannibal-Bach, Torben Helledie, Christina Fenger, Marianne Due, Zane Berzina, Reinhard Neubert, John Chemnitz, Bente Finsen, Anders Clemmensen, Johannes Wilbertz, Henrik Saxtorph, Jens Knudsen, Luis Bagatolli, Susanne Mandrup

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

The acyl-CoA binding protein (ACBP) is a 10 kDa intracellular protein expressed in all eukaryotic species. Mice with targeted disruption of Acbp (ACBP(-/-) mice) are viable and fertile but present a visible skin and fur phenotype characterized by greasy fur and development of alopecia and scaling with age. Morphology and development of skin and appendages are normal in ACBP(-/-) mice; however, the stratum corneum display altered biophysical properties with reduced proton activity and decreased water content. Mass spectrometry analyses of lipids from epidermis and stratum corneum of ACBP(+/+) and ACBP(-/-) mice showed very similar composition, except for a significant and specific decrease in the very long chain free fatty acids (VLC-FFA) in stratum corneum of ACBP(-/-) mice. This finding indicates that ACBP is critically involved in the processes that lead to production of stratum corneum VLC-FFAs via complex phospholipids in the lamellar bodies. Importantly, we show that ACBP(-/-) mice display a ∼50% increased transepidermal water loss compared with ACBP(+/+) mice. Furthermore, skin and fur sebum monoalkyl diacylglycerol (MADAG) levels are significantly increased, suggesting that ACBP limits MADAG synthesis in sebaceous glands. In summary, our study shows that ACBP is required for production of VLC-FFA for stratum corneum and for maintaining normal epidermal barrier function.
OriginalsprogEngelsk
TidsskriftJournal of Lipid Research
Vol/bind53
Udgave nummer10
Sider (fra-til)2162-74
Antal sider13
ISSN0022-2275
DOI
StatusUdgivet - 2012

Fingeraftryk

Diazepam Binding Inhibitor
Skin
Diglycerides
Nonesterified Fatty Acids
Sebum
Sebaceous Glands
Epidermis
Water content
Mass spectrometry
Protons

Citer dette

Bloksgaard, Maria ; Bek, Signe ; Marcher, Ann-Britt ; Neess, Ditte ; Brewer, Jonathan ; Hannibal-Bach, Hans Kristian ; Helledie, Torben ; Fenger, Christina ; Due, Marianne ; Berzina, Zane ; Neubert, Reinhard ; Chemnitz, John ; Finsen, Bente ; Clemmensen, Anders ; Wilbertz, Johannes ; Saxtorph, Henrik ; Knudsen, Jens ; Bagatolli, Luis ; Mandrup, Susanne. / The acyl-CoA binding protein is required for normal epidermal barrier function in mice. I: Journal of Lipid Research. 2012 ; Bind 53, Nr. 10. s. 2162-74.
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title = "The acyl-CoA binding protein is required for normal epidermal barrier function in mice",
abstract = "The acyl-CoA binding protein (ACBP) is a 10 kDa intracellular protein expressed in all eukaryotic species. Mice with targeted disruption of Acbp (ACBP(-/-) mice) are viable and fertile but present a visible skin and fur phenotype characterized by greasy fur and development of alopecia and scaling with age. Morphology and development of skin and appendages are normal in ACBP(-/-) mice; however, the stratum corneum display altered biophysical properties with reduced proton activity and decreased water content. Mass spectrometry analyses of lipids from epidermis and stratum corneum of ACBP(+/+) and ACBP(-/-) mice showed very similar composition, except for a significant and specific decrease in the very long chain free fatty acids (VLC-FFA) in stratum corneum of ACBP(-/-) mice. This finding indicates that ACBP is critically involved in the processes that lead to production of stratum corneum VLC-FFAs via complex phospholipids in the lamellar bodies. Importantly, we show that ACBP(-/-) mice display a ∼50{\%} increased transepidermal water loss compared with ACBP(+/+) mice. Furthermore, skin and fur sebum monoalkyl diacylglycerol (MADAG) levels are significantly increased, suggesting that ACBP limits MADAG synthesis in sebaceous glands. In summary, our study shows that ACBP is required for production of VLC-FFA for stratum corneum and for maintaining normal epidermal barrier function.",
author = "Maria Bloksgaard and Signe Bek and Ann-Britt Marcher and Ditte Neess and Jonathan Brewer and Hannibal-Bach, {Hans Kristian} and Torben Helledie and Christina Fenger and Marianne Due and Zane Berzina and Reinhard Neubert and John Chemnitz and Bente Finsen and Anders Clemmensen and Johannes Wilbertz and Henrik Saxtorph and Jens Knudsen and Luis Bagatolli and Susanne Mandrup",
year = "2012",
doi = "10.1194/jlr.M029553",
language = "English",
volume = "53",
pages = "2162--74",
journal = "Journal of Lipid Research",
issn = "0022-2275",
publisher = "American Society for Biochemistry and Molecular Biology, Inc.",
number = "10",

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The acyl-CoA binding protein is required for normal epidermal barrier function in mice. / Bloksgaard, Maria; Bek, Signe; Marcher, Ann-Britt; Neess, Ditte; Brewer, Jonathan; Hannibal-Bach, Hans Kristian; Helledie, Torben; Fenger, Christina; Due, Marianne; Berzina, Zane; Neubert, Reinhard; Chemnitz, John; Finsen, Bente; Clemmensen, Anders; Wilbertz, Johannes; Saxtorph, Henrik; Knudsen, Jens; Bagatolli, Luis; Mandrup, Susanne.

I: Journal of Lipid Research, Bind 53, Nr. 10, 2012, s. 2162-74.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - The acyl-CoA binding protein is required for normal epidermal barrier function in mice

AU - Bloksgaard, Maria

AU - Bek, Signe

AU - Marcher, Ann-Britt

AU - Neess, Ditte

AU - Brewer, Jonathan

AU - Hannibal-Bach, Hans Kristian

AU - Helledie, Torben

AU - Fenger, Christina

AU - Due, Marianne

AU - Berzina, Zane

AU - Neubert, Reinhard

AU - Chemnitz, John

AU - Finsen, Bente

AU - Clemmensen, Anders

AU - Wilbertz, Johannes

AU - Saxtorph, Henrik

AU - Knudsen, Jens

AU - Bagatolli, Luis

AU - Mandrup, Susanne

PY - 2012

Y1 - 2012

N2 - The acyl-CoA binding protein (ACBP) is a 10 kDa intracellular protein expressed in all eukaryotic species. Mice with targeted disruption of Acbp (ACBP(-/-) mice) are viable and fertile but present a visible skin and fur phenotype characterized by greasy fur and development of alopecia and scaling with age. Morphology and development of skin and appendages are normal in ACBP(-/-) mice; however, the stratum corneum display altered biophysical properties with reduced proton activity and decreased water content. Mass spectrometry analyses of lipids from epidermis and stratum corneum of ACBP(+/+) and ACBP(-/-) mice showed very similar composition, except for a significant and specific decrease in the very long chain free fatty acids (VLC-FFA) in stratum corneum of ACBP(-/-) mice. This finding indicates that ACBP is critically involved in the processes that lead to production of stratum corneum VLC-FFAs via complex phospholipids in the lamellar bodies. Importantly, we show that ACBP(-/-) mice display a ∼50% increased transepidermal water loss compared with ACBP(+/+) mice. Furthermore, skin and fur sebum monoalkyl diacylglycerol (MADAG) levels are significantly increased, suggesting that ACBP limits MADAG synthesis in sebaceous glands. In summary, our study shows that ACBP is required for production of VLC-FFA for stratum corneum and for maintaining normal epidermal barrier function.

AB - The acyl-CoA binding protein (ACBP) is a 10 kDa intracellular protein expressed in all eukaryotic species. Mice with targeted disruption of Acbp (ACBP(-/-) mice) are viable and fertile but present a visible skin and fur phenotype characterized by greasy fur and development of alopecia and scaling with age. Morphology and development of skin and appendages are normal in ACBP(-/-) mice; however, the stratum corneum display altered biophysical properties with reduced proton activity and decreased water content. Mass spectrometry analyses of lipids from epidermis and stratum corneum of ACBP(+/+) and ACBP(-/-) mice showed very similar composition, except for a significant and specific decrease in the very long chain free fatty acids (VLC-FFA) in stratum corneum of ACBP(-/-) mice. This finding indicates that ACBP is critically involved in the processes that lead to production of stratum corneum VLC-FFAs via complex phospholipids in the lamellar bodies. Importantly, we show that ACBP(-/-) mice display a ∼50% increased transepidermal water loss compared with ACBP(+/+) mice. Furthermore, skin and fur sebum monoalkyl diacylglycerol (MADAG) levels are significantly increased, suggesting that ACBP limits MADAG synthesis in sebaceous glands. In summary, our study shows that ACBP is required for production of VLC-FFA for stratum corneum and for maintaining normal epidermal barrier function.

U2 - 10.1194/jlr.M029553

DO - 10.1194/jlr.M029553

M3 - Journal article

VL - 53

SP - 2162

EP - 2174

JO - Journal of Lipid Research

JF - Journal of Lipid Research

SN - 0022-2275

IS - 10

ER -