@article{c495b290cf0311deb0fe000ea68e967b,
title = "The acquisition of cytokine responsiveness by murine B cells: A role for antigen and IL-5 in the induction of IL-2 receptors",
abstract = "The mechanism whereby small resting (high buoyant density) murine B cells are induced to express interleukin-2 receptors (IL-2R) and to respond to IL-2 was addressed by staining with anti-IL-2R alpha and -IL-2R beta monoclonal antibodies (mAb), and using receptor-specific cDNA probes. Resting B cells expressed undetectable levels of both IL-2R alpha and beta chains on their surface and did not respond to IL-2, even at supra-physiological concentrations. Sepharose-coupled, but not streptavidin-cross-linked, plastic-adsorbed or soluble, anti-mu up-regulated the expression of IL-2R alpha and beta chains and mRNA to levels comparable to those seen in activated T cells. Anti-mu-stimulated B cells responded to IL-2 by incorporation of [3H]thymidine and high rate immunoglobulin (Ig) secretion. Both IL-5 (at optimal concentration) and suboptimal lipopolysaccharide (LPS; 20 ng/ml) induced surface expression of IL-2R alpha. The level of expression induced by IL-5 was equivalent to that on anti-Ig-activated B cells. Neither stimulus induced detectable expression of IL-2R beta, and neither induced B cells to respond to IL-2. IL-2R alpha expression was strongly enhanced, and low levels of IL-2R beta staining and mRNA were induced by the combination of LPS plus IL-5. LPS+IL-5-treated B cells responded to IL-2 by Ig secretion. This indicates that B cells regulate their responsiveness to IL-2 similarly to T cells, via the combined level of expression of IL-2R beta and IL-2R alpha. The synergy between IL-5 and LPS for B-cell responses shows a requirement for complementary stimuli such as would be provided by cytokines, and either cellular interaction or antigen recognition in regulation of B-cell responsiveness to IL-2.",
keywords = "Animals, B-Lymphocytes, Cells, Cultured, Drug Synergism, Female, Interleukin-2, Interleukin-5, Lipopolysaccharides, Lymphocyte Activation, Mice, Mice, Inbred C57BL, RNA, Messenger, Receptors, Antigen, B-Cell, Receptors, Interleukin-2, Up-Regulation",
author = "J Poudrier and T Owens",
year = "1994",
month = mar,
day = "1",
language = "English",
volume = "81",
pages = "373--80",
journal = "Immunology",
issn = "0019-2805",
publisher = "Wiley",
number = "3",
}