Tetrameric and polymeric silver complexes of the omeprazole scaffold; synthesis, structure, in vitro and in vivo antimicrobial activities and DNA interaction

Vibe Jakobsen; Livia Viganor; Alfonso Blanco-Fernández; Orla Howe; Michael Devereux; Christine J. McKenzie; Vickie McKee

doi:10.1016/j.jinorgbio.2018.05.018

Tetrameric and polymeric silver complexes of the omeprazole scaffold; synthesis, structure, in vitro and in vivo antimicrobial activities and DNA interaction

Vibe Jakobsen, Livia Viganor, Alfonso Blanco-Fernández, Orla Howe, Michael Devereux, Christine J. McKenzie, Vickie McKee^*

^*Kontaktforfatter

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Abstract

Two complexes [AgI(pmtbH)]₄ (1) and {[Ag₄(pmtbH)₄(NO₃)₄·2X}_n (2) (where pmtbH is 2-[(2-pyridinylmethyl)thio]-1H-benzimidazole and X is H₂O or MeOH) were synthesised and structurally characterised. Complex 2 showed therapeutic potential against Candida albicans, Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa but complex 1 did not show significant activity in vitro. Further in vivo studies using larvae of the insect Galleria mellonella indicated that complex 2 significantly stimulates the immune system and that pre-treatment with the complex offers appreciable protection against all three bacteria. Real-time flow cytometry data support the observed antimicrobial profile of complex 2 and suggest the antimicrobial response may be linked to a form of bacterial programmed cell death (PCD). Complex 2 was found to interact with DNA in the bacterial and fungal cells but it did not cleave plasmid DNA isolated from the three bacteria.

Originalsprog	Engelsk
Tidsskrift	Journal of Inorganic Biochemistry
Vol/bind	186
Sider (fra-til)	317-328
ISSN	0162-0134
DOI	https://doi.org/10.1016/j.jinorgbio.2018.05.018
Status	Udgivet - 1. sep. 2018

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10.1016/j.jinorgbio.2018.05.018

Open Access VersionAccepteret manuskript, 1,84 MBLicens: CC BY-NC-ND

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title = "Tetrameric and polymeric silver complexes of the omeprazole scaffold; synthesis, structure, in vitro and in vivo antimicrobial activities and DNA interaction",

abstract = "Two complexes [AgI(pmtbH)]4 (1) and {[Ag4(pmtbH)4(NO3)4·2X}n (2) (where pmtbH is 2-[(2-pyridinylmethyl)thio]-1H-benzimidazole and X is H2O or MeOH) were synthesised and structurally characterised. Complex 2 showed therapeutic potential against Candida albicans, Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa but complex 1 did not show significant activity in vitro. Further in vivo studies using larvae of the insect Galleria mellonella indicated that complex 2 significantly stimulates the immune system and that pre-treatment with the complex offers appreciable protection against all three bacteria. Real-time flow cytometry data support the observed antimicrobial profile of complex 2 and suggest the antimicrobial response may be linked to a form of bacterial programmed cell death (PCD). Complex 2 was found to interact with DNA in the bacterial and fungal cells but it did not cleave plasmid DNA isolated from the three bacteria.",

keywords = "Antimicrobial, Flow cytometry, Galleria mellonella, Omeprazole, Silver(I)",

author = "Vibe Jakobsen and Livia Viganor and Alfonso Blanco-Fern{\'a}ndez and Orla Howe and Michael Devereux and McKenzie, {Christine J.} and Vickie McKee",

year = "2018",

month = sep,

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doi = "10.1016/j.jinorgbio.2018.05.018",

language = "English",

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Tetrameric and polymeric silver complexes of the omeprazole scaffold; synthesis, structure, in vitro and in vivo antimicrobial activities and DNA interaction. / Jakobsen, Vibe; Viganor, Livia; Blanco-Fernández, Alfonso et al.
I: Journal of Inorganic Biochemistry, Bind 186, 01.09.2018, s. 317-328.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

TY - JOUR

T1 - Tetrameric and polymeric silver complexes of the omeprazole scaffold; synthesis, structure, in vitro and in vivo antimicrobial activities and DNA interaction

AU - Jakobsen, Vibe

AU - Viganor, Livia

AU - Blanco-Fernández, Alfonso

AU - Howe, Orla

AU - Devereux, Michael

AU - McKenzie, Christine J.

AU - McKee, Vickie

PY - 2018/9/1

Y1 - 2018/9/1

N2 - Two complexes [AgI(pmtbH)]4 (1) and {[Ag4(pmtbH)4(NO3)4·2X}n (2) (where pmtbH is 2-[(2-pyridinylmethyl)thio]-1H-benzimidazole and X is H2O or MeOH) were synthesised and structurally characterised. Complex 2 showed therapeutic potential against Candida albicans, Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa but complex 1 did not show significant activity in vitro. Further in vivo studies using larvae of the insect Galleria mellonella indicated that complex 2 significantly stimulates the immune system and that pre-treatment with the complex offers appreciable protection against all three bacteria. Real-time flow cytometry data support the observed antimicrobial profile of complex 2 and suggest the antimicrobial response may be linked to a form of bacterial programmed cell death (PCD). Complex 2 was found to interact with DNA in the bacterial and fungal cells but it did not cleave plasmid DNA isolated from the three bacteria.

AB - Two complexes [AgI(pmtbH)]4 (1) and {[Ag4(pmtbH)4(NO3)4·2X}n (2) (where pmtbH is 2-[(2-pyridinylmethyl)thio]-1H-benzimidazole and X is H2O or MeOH) were synthesised and structurally characterised. Complex 2 showed therapeutic potential against Candida albicans, Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa but complex 1 did not show significant activity in vitro. Further in vivo studies using larvae of the insect Galleria mellonella indicated that complex 2 significantly stimulates the immune system and that pre-treatment with the complex offers appreciable protection against all three bacteria. Real-time flow cytometry data support the observed antimicrobial profile of complex 2 and suggest the antimicrobial response may be linked to a form of bacterial programmed cell death (PCD). Complex 2 was found to interact with DNA in the bacterial and fungal cells but it did not cleave plasmid DNA isolated from the three bacteria.

KW - Antimicrobial

KW - Flow cytometry

KW - Galleria mellonella

KW - Omeprazole

KW - Silver(I)

U2 - 10.1016/j.jinorgbio.2018.05.018

DO - 10.1016/j.jinorgbio.2018.05.018

M3 - Journal article

C2 - 30025225

AN - SCOPUS:85049857921

SN - 0162-0134

VL - 186

SP - 317

EP - 328

JO - Journal of Inorganic Biochemistry

JF - Journal of Inorganic Biochemistry

ER -

Tetrameric and polymeric silver complexes of the omeprazole scaffold; synthesis, structure, in vitro and in vivo antimicrobial activities and DNA interaction

Abstract

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