Template Directed Oligomer Ligation in Eutectic Phases in Water-Ice

Mark Dörr, Philipp M. G. Löffler, Rafal Wieczorek, Pierre-Alain Monnard

Publikation: Konferencebidrag uden forlag/tidsskriftPosterForskning

Resumé

Eutectic phases of water-ice are protective micro-environments for the non-enzymatic, metal ion mediated
polymerization of imidazole-activated ribonucleotides (Monnard 2008). Polymers of up to 30-mer lengths
can herein be achieved. Even longer polymers can be obtained by adding activated monomers or short
oligomers to a solution over several freeze-thawing cycles. The final length is determined by the balancing
rates of condensation and hydrolysis. A certain critical length of about 25 to 30 oligonucleotides is required
to show catalytic activity (Scott 1995). This length can be much quicker achieved, if small, activated,
oligonucleotides are ligated on a template. A template directed ligation can lead to autocatalytic or cross-
catalytic replication and thus maintain a certain pool of catalyitc species. Important for these processes is a
destabilization of the formed douplex/multiplex to overcome product inhibition. The latest results of our
template directed ligation experiments in the eutectic ice phase are presented. Different activation strategies
are compared and an outlook towards applications in molecular evolution and artifical cell systems
(« protocells ») will be given.
Figure 1. (a) Reaction scheme of the condensation reaction of two oligoribonucleotides : The leaving group in this
example is imidazole. (b) Illustration of a possible spatial arrangement of a template (15nt) directed ligation. The 7-mer
is activated with imidazole at the 5' phosphate (apical moiety in the middle of the construct).
References
Monnard P, Szostak JW (2008). Metal-ion catalyzed polymerization in the eutectic phase in water-ice: A possible
approach to template-directed RNA polymerization. J.Inorg.Biochem., 102(5-6):1104-1111.
Scott WG, Finch JT, Klug A (1995). The crystal structure of an AII-RNA hammerhead ribozyme: A proposed
mechanism for RNA catalytic cleavage. Cell, 81(7):991-1002
OriginalsprogEngelsk
Publikationsdato3. jul. 2011
Antal sider1
StatusUdgivet - 3. jul. 2011

Fingeraftryk

Ice
Oligomers
Eutectics
Oligonucleotides
Metal ions
Water
Polymers
Polymerization
Oligoribonucleotides
RNA
Ribonucleotides
Catalytic RNA
Thawing
Condensation reactions
Condensation
Hydrolysis
Catalyst activity
Monomers
Crystal structure
Chemical activation

Citer dette

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title = "Template Directed Oligomer Ligation in Eutectic Phases in Water-Ice",
abstract = "Eutectic phases of water-ice are protective micro-environments for the non-enzymatic, metal ion mediatedpolymerization of imidazole-activated ribonucleotides (Monnard 2008). Polymers of up to 30-mer lengthscan herein be achieved. Even longer polymers can be obtained by adding activated monomers or shortoligomers to a solution over several freeze-thawing cycles. The final length is determined by the balancingrates of condensation and hydrolysis. A certain critical length of about 25 to 30 oligonucleotides is requiredto show catalytic activity (Scott 1995). This length can be much quicker achieved, if small, activated,oligonucleotides are ligated on a template. A template directed ligation can lead to autocatalytic or cross-catalytic replication and thus maintain a certain pool of catalyitc species. Important for these processes is adestabilization of the formed douplex/multiplex to overcome product inhibition. The latest results of ourtemplate directed ligation experiments in the eutectic ice phase are presented. Different activation strategiesare compared and an outlook towards applications in molecular evolution and artifical cell systems(« protocells ») will be given.Figure 1. (a) Reaction scheme of the condensation reaction of two oligoribonucleotides : The leaving group in thisexample is imidazole. (b) Illustration of a possible spatial arrangement of a template (15nt) directed ligation. The 7-meris activated with imidazole at the 5' phosphate (apical moiety in the middle of the construct).ReferencesMonnard P, Szostak JW (2008). Metal-ion catalyzed polymerization in the eutectic phase in water-ice: A possibleapproach to template-directed RNA polymerization. J.Inorg.Biochem., 102(5-6):1104-1111.Scott WG, Finch JT, Klug A (1995). The crystal structure of an AII-RNA hammerhead ribozyme: A proposedmechanism for RNA catalytic cleavage. Cell, 81(7):991-1002",
keywords = "oligonucleotides, RNA, Polymers, Eutectic phase in water-ice, Origin of Life",
author = "Mark D{\"o}rr and L{\"o}ffler, {Philipp M. G.} and Rafal Wieczorek and Pierre-Alain Monnard",
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}

Template Directed Oligomer Ligation in Eutectic Phases in Water-Ice. / Dörr, Mark; Löffler, Philipp M. G.; Wieczorek, Rafal; Monnard, Pierre-Alain.

2011.

Publikation: Konferencebidrag uden forlag/tidsskriftPosterForskning

TY - CONF

T1 - Template Directed Oligomer Ligation in Eutectic Phases in Water-Ice

AU - Dörr, Mark

AU - Löffler, Philipp M. G.

AU - Wieczorek, Rafal

AU - Monnard, Pierre-Alain

PY - 2011/7/3

Y1 - 2011/7/3

N2 - Eutectic phases of water-ice are protective micro-environments for the non-enzymatic, metal ion mediatedpolymerization of imidazole-activated ribonucleotides (Monnard 2008). Polymers of up to 30-mer lengthscan herein be achieved. Even longer polymers can be obtained by adding activated monomers or shortoligomers to a solution over several freeze-thawing cycles. The final length is determined by the balancingrates of condensation and hydrolysis. A certain critical length of about 25 to 30 oligonucleotides is requiredto show catalytic activity (Scott 1995). This length can be much quicker achieved, if small, activated,oligonucleotides are ligated on a template. A template directed ligation can lead to autocatalytic or cross-catalytic replication and thus maintain a certain pool of catalyitc species. Important for these processes is adestabilization of the formed douplex/multiplex to overcome product inhibition. The latest results of ourtemplate directed ligation experiments in the eutectic ice phase are presented. Different activation strategiesare compared and an outlook towards applications in molecular evolution and artifical cell systems(« protocells ») will be given.Figure 1. (a) Reaction scheme of the condensation reaction of two oligoribonucleotides : The leaving group in thisexample is imidazole. (b) Illustration of a possible spatial arrangement of a template (15nt) directed ligation. The 7-meris activated with imidazole at the 5' phosphate (apical moiety in the middle of the construct).ReferencesMonnard P, Szostak JW (2008). Metal-ion catalyzed polymerization in the eutectic phase in water-ice: A possibleapproach to template-directed RNA polymerization. J.Inorg.Biochem., 102(5-6):1104-1111.Scott WG, Finch JT, Klug A (1995). The crystal structure of an AII-RNA hammerhead ribozyme: A proposedmechanism for RNA catalytic cleavage. Cell, 81(7):991-1002

AB - Eutectic phases of water-ice are protective micro-environments for the non-enzymatic, metal ion mediatedpolymerization of imidazole-activated ribonucleotides (Monnard 2008). Polymers of up to 30-mer lengthscan herein be achieved. Even longer polymers can be obtained by adding activated monomers or shortoligomers to a solution over several freeze-thawing cycles. The final length is determined by the balancingrates of condensation and hydrolysis. A certain critical length of about 25 to 30 oligonucleotides is requiredto show catalytic activity (Scott 1995). This length can be much quicker achieved, if small, activated,oligonucleotides are ligated on a template. A template directed ligation can lead to autocatalytic or cross-catalytic replication and thus maintain a certain pool of catalyitc species. Important for these processes is adestabilization of the formed douplex/multiplex to overcome product inhibition. The latest results of ourtemplate directed ligation experiments in the eutectic ice phase are presented. Different activation strategiesare compared and an outlook towards applications in molecular evolution and artifical cell systems(« protocells ») will be given.Figure 1. (a) Reaction scheme of the condensation reaction of two oligoribonucleotides : The leaving group in thisexample is imidazole. (b) Illustration of a possible spatial arrangement of a template (15nt) directed ligation. The 7-meris activated with imidazole at the 5' phosphate (apical moiety in the middle of the construct).ReferencesMonnard P, Szostak JW (2008). Metal-ion catalyzed polymerization in the eutectic phase in water-ice: A possibleapproach to template-directed RNA polymerization. J.Inorg.Biochem., 102(5-6):1104-1111.Scott WG, Finch JT, Klug A (1995). The crystal structure of an AII-RNA hammerhead ribozyme: A proposedmechanism for RNA catalytic cleavage. Cell, 81(7):991-1002

KW - oligonucleotides

KW - RNA

KW - Polymers

KW - Eutectic phase in water-ice

KW - Origin of Life

M3 - Poster

ER -