@article{a2604310cf0411deb0fe000ea68e967b,
title = "T cell antigen receptor expression by subsets of Ly-2-L3T4- (CD8-CD4-) thymocytes",
abstract = "The V beta 8-specific mAb F23.1 and KJ16 were used as fluorescent stains to test for TCR expression on the surface of subpopulations of early, CD4-CD8- (L3T4-Ly-2-) thymocytes from adult CBA mice. A surprisingly high proportion (27\%) of Ly-2-L3T4- thymocytes were strongly F23.1 and KJ16 positive. No positive cells were detected among Ly-2-L3T4- thymocytes from V beta 8-negative SJL mice. In contrast to the adult thymus, Ly-2-L3T4- cells from embryonic CBA thymus lacked F23.1-positive cells. Subsets of adult CBA Ly-2-L3T4- thymocytes were separated to determine which expressed V beta 8. The major subset, Ly-1 low B2A2-M1/69+Thy-1+Pgp-1-, representing a phenotype similar to embryonic Ly-2-L3T4- thymocytes and the phenotype commonly isolated from adult thymocytes as Ly-1 {"}dull,{"} lacked cells strongly positive for F23.1. In contrast, a series of subsets of adult CBA Ly-2-L3T4- thymocytes which were B2A2-M1/69- and Pgp-1+ all included strongly F23.1-positive cells. A minor subset, negative for most markers except Pgp-1 and presumed on the basis of this phenotype and some reconstitution studies to include the earliest intrathymic precursors, contained 28\% F23.1-positive cells. However, no F.23.1-positive cells were detected in equivalent {"}prethymic{"} populations from bone marrow or from athymic mouse spleen. The subsets of Ly-2-L3T4- thymocytes which were Ly-1 high, B2A2-M1/69-, and Pgp-1+ all contained about 70\% F23.1-positive cells, indicating a V beta 8 usage much higher than the mature T cell average. These results indicate that a series of distinct developmental events have occurred within these CD4-CD8- thymocytes previously considered as a single group of early precursor cells, and that some aspects of repertoire selection may be occurring amongst thymocytes which lack CD4 or CD8.",
keywords = "Aging, Animals, Antibodies, Monoclonal, Antigens, Differentiation, T-Lymphocyte, Antigens, Ly, Antigens, Surface, Cell Line, Embryo, Mammalian, Fluorescent Dyes, Hematopoietic Stem Cells, Male, Membrane Glycoproteins, Mice, Mice, Inbred CBA, Mice, Nude, Phenotype, Receptors, Antigen, T-Cell, Receptors, Lymphocyte Homing, Staining and Labeling, T-Lymphocytes",
author = "A Wilson and T Ewing and T Owens and R Scollay and K Shortman",
year = "1988",
month = mar,
day = "1",
doi = "10.4049/jimmunol.140.5.1470",
language = "English",
volume = "140",
pages = "1470--6",
journal = "The Journal of Immunology",
issn = "0022-1767",
publisher = "Oxford University Press",
number = "5",
}