Synthetic LNA/DNA nano-scaffolds for highly efficient diagnostics of nucleic acids and autoimmune antibodies

Irina Kira Astakhova*

*Kontaktforfatter for dette arbejde

Publikation: Kapitel i bog/rapport/konference-proceedingKonferencebidrag i proceedingsForskningpeer review

Abstrakt

Herein novel fluorescent oligonucleotides for homogeneous (all-in-solution) detection of nucleic acids and autoimmune antibodies (autoantibodies) are described. The probes are prepared by highly efficient copper-catalyzed click chemistry between novel alkyne-modified locked nucleic acid (LNA) strands and a series of fluorescent azides. The multiply labeled fluorescent LNA/DNA probes prepared herein generally display high binding affinity to complementary DNA/RNA, high quantum yields and, hence, high fluorescence brightness values. With the novel fluorescent probes, specific sensing of the monoclonal human autoantibody is achieved. It makes the novel "clickable" LNA/DNA complexes a very promising tool in molecular diagnostics of both nucleic acids and autoantibodies against DNA. The latter are produced under several autoimmune conditions including antiphospholipide syndrome and systemic lupus erythematosus.

OriginalsprogEngelsk
TitelNanotechnology 2014 : Technical Proceedings of the 2014 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2014
Vol/bind2
ForlagCRC Press
Publikationsdato2014
Sider181-184
ISBN (Trykt)978-148225827-1
StatusUdgivet - 2014
BegivenhedNanotechnology Conference and Expo: NSTI-Nanotech 2014 - Washington, DC, USA
Varighed: 15. jun. 201418. jun. 2014

Konference

KonferenceNanotechnology Conference and Expo
LandUSA
ByWashington, DC
Periode15/06/201418/06/2014

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