Synthesis and Thermal Denaturation Studies of Conformationally Restricted 3'-C-Ethynyl-3'-O,4'-C-Methyleneribonucleotides

Patrick J. Hrdlicka, T.S. Kumar, Jesper Wengel

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

Incorporation of conformationally restricted 3′‐C‐ethynyl‐3′‐O,4′‐C‐methyleneribonucleotide X monomers into oligodeoxyribonucleotides results in large decreases in affinity towards complementary DNA, whereas significantly more stable duplexes are formed with RNA complements. RNA selectivity becomes more pronounced upon multiple incorporations of X monomers. Increased thermal stability is observed upon positioning of two X monomers in a +1 zipper interstrand motif. (© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2005)
OriginalsprogEngelsk
TidsskriftEuropean Journal of Organic Chemistry
Vol/bind2005
Udgave nummer24
Sider (fra-til)5184-5188
ISSN1434-193X
DOI
StatusUdgivet - 2005

Fingeraftryk

Denaturation
Monomers
RNA
Oligodeoxyribonucleotides
Fasteners
Thermodynamic stability
Complementary DNA
Hot Temperature

Citer dette

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title = "Synthesis and Thermal Denaturation Studies of Conformationally Restricted 3'-C-Ethynyl-3'-O,4'-C-Methyleneribonucleotides",
abstract = "Incorporation of conformationally restricted 3′‐C‐ethynyl‐3′‐O,4′‐C‐methyleneribonucleotide X monomers into oligodeoxyribonucleotides results in large decreases in affinity towards complementary DNA, whereas significantly more stable duplexes are formed with RNA complements. RNA selectivity becomes more pronounced upon multiple incorporations of X monomers. Increased thermal stability is observed upon positioning of two X monomers in a +1 zipper interstrand motif. ({\circledC} Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2005)",
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doi = "10.1002/ejoc.200500379",
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Synthesis and Thermal Denaturation Studies of Conformationally Restricted 3'-C-Ethynyl-3'-O,4'-C-Methyleneribonucleotides. / Hrdlicka, Patrick J.; Kumar, T.S.; Wengel, Jesper.

I: European Journal of Organic Chemistry, Bind 2005, Nr. 24, 2005, s. 5184-5188.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Synthesis and Thermal Denaturation Studies of Conformationally Restricted 3'-C-Ethynyl-3'-O,4'-C-Methyleneribonucleotides

AU - Hrdlicka, Patrick J.

AU - Kumar, T.S.

AU - Wengel, Jesper

PY - 2005

Y1 - 2005

N2 - Incorporation of conformationally restricted 3′‐C‐ethynyl‐3′‐O,4′‐C‐methyleneribonucleotide X monomers into oligodeoxyribonucleotides results in large decreases in affinity towards complementary DNA, whereas significantly more stable duplexes are formed with RNA complements. RNA selectivity becomes more pronounced upon multiple incorporations of X monomers. Increased thermal stability is observed upon positioning of two X monomers in a +1 zipper interstrand motif. (© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2005)

AB - Incorporation of conformationally restricted 3′‐C‐ethynyl‐3′‐O,4′‐C‐methyleneribonucleotide X monomers into oligodeoxyribonucleotides results in large decreases in affinity towards complementary DNA, whereas significantly more stable duplexes are formed with RNA complements. RNA selectivity becomes more pronounced upon multiple incorporations of X monomers. Increased thermal stability is observed upon positioning of two X monomers in a +1 zipper interstrand motif. (© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2005)

U2 - 10.1002/ejoc.200500379

DO - 10.1002/ejoc.200500379

M3 - Journal article

VL - 2005

SP - 5184

EP - 5188

JO - European Journal of Organic Chemistry

JF - European Journal of Organic Chemistry

SN - 1434-193X

IS - 24

ER -