Study on the antioxidant activity and membrane interaction of a multiple antioxidant

Kasia Jodko-Jodko-Piórecka, Beate Klösgen, Grzegorz Litwinienko

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Abstrakt

Catecholamines, including dopamine, have a role in the transduction of nervous stimuli. Apart from that, they might behave as
endogenous phenolic antioxidants protecting the neuronal tissue from deleterious effects of oxidative stress. Results from our
preliminary study1 even indicate a synergistic effect: an interplay of catecholamines with other molecules seems to enhance
their antioxidant activity (e.g. 3,4-dihydroxyphenylalanine - L-DOPA -, and 2,2,5,7,8-pentamethyl-6-hydroxychroman - PMHC -,
an analogue of α-tocopherol). Addition of small amounts of catecholamines suppresses or largely inhibits the peroxidation of
lipids2. The understanding of the mechanism of antioxidant action of catecholamines and their interplay with other antioxidants
in lipid membranes requires interdisciplinary research on the kinetics and thermodynamics of antioxidant(s)/membrane
interactions.
Here we report kinetic data that, as a prerequisite, verify the antioxidant activity of dopamine by measuring its scavenging
activity against peroxyl radicals in a heterogeneous lipid/water system and give evidence about the synergistic effect between
dopamine and PMHC. We also present preliminary results from ongoing experiments on the interaction between dopamine,
PMHC and model lipid membranes by microcalorimetry (DSC, ITC). The calorimetric studies are performed on unilamellar
vesicles assembled from 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) as a model lipid membrane. As a hypothesis
for the dopamine/PMHC antioxidative co-operation we propose that dopamine acts mainly as a scavenger of radicals near the
membrane surface while PMHC is scavenging the peroxyl radicals within the membrane.
References
1) Jodko, K.; Kowalewska, E.; Sikora, B.; Litwinienko, G. Free Radic. Res. 2009, 43, 58
2) Liu, J; Mori, A. Arch. Biochem. Biophys. 1993, 302, 118
OriginalsprogEngelsk
Publikationsdato3. aug. 2011
StatusUdgivet - 3. aug. 2011
Begivenhed43rd IUPAC 2011 World Chemistry Congress; Chemistry Bridging Innovation Among the Americas and the World - San Juan, Puerto Rico
Varighed: 30. jul. 20117. aug. 2011

Konference

Konference43rd IUPAC 2011 World Chemistry Congress; Chemistry Bridging Innovation Among the Americas and the World
LandPuerto Rico
BySan Juan
Periode30/07/201107/08/2011

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