Stereological estimation of nuclear volume in benign melanocytic lesions and cutaneous malignant melanomas

Flemming Brandt Sørensen

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

Recent developments in stereology have made it possible to obtain unbiased, objective estimates of the volume weighted mean nuclear volume (vV) from ordinary histological sections, without any assumptions concerning nuclear shape. The aim of this study was to gather baseline data of nuclear vV in melanocytic cutaneous tumors and to compare these with estimates of nuclear volume fraction and with traditional two-dimensional morphometric estimates of nuclear profile area, nuclear density, and mitotic index. Routinely processed, paraffin embedded tissue specimens from 47 malignant melanomas and 76 noninvasive melanocytic cutaneous tumors were investigated retrospectively. vV clearly distinguished between noninvasive (average vV = 122 microns 3) and invasive lesions (average vV = 246 microns 3). Most of the patients with malignant melanomas showing an overlap of nuclear vV with benign lesions had a favorable prognosis. No significant differences in vV could be demonstrated among different noninvasive tumor types. Two-dimensional estimates only distinguished benign from malignant tumors with considerable overlap and with significantly varying influence from other factors among different benign lesional types. The variance associated with estimates of vV disclosed a linear relationship to vV, whereas the relative variation seemed to be constant. Tumor heterogeneity was without significant influence on estimates of nuclear vV. It is concluded that nuclear vV is a superior, efficient, and sensitive estimator for distinguishing between melanocytic cutaneous tumors showing different biological behavior, well-suited for objective malignancy grading.

OriginalsprogEngelsk
TidsskriftAmerican Journal of Dermatopathology
Vol/bind11
Udgave nummer6
Sider (fra-til)517-27
Antal sider11
ISSN0193-1091
StatusUdgivet - dec. 1989

Fingeraftryk

Neoplasms
Skin
Melanoma
Mitotic Index
Cutaneous Malignant Melanoma
Paraffin

Citer dette

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title = "Stereological estimation of nuclear volume in benign melanocytic lesions and cutaneous malignant melanomas",
abstract = "Recent developments in stereology have made it possible to obtain unbiased, objective estimates of the volume weighted mean nuclear volume (vV) from ordinary histological sections, without any assumptions concerning nuclear shape. The aim of this study was to gather baseline data of nuclear vV in melanocytic cutaneous tumors and to compare these with estimates of nuclear volume fraction and with traditional two-dimensional morphometric estimates of nuclear profile area, nuclear density, and mitotic index. Routinely processed, paraffin embedded tissue specimens from 47 malignant melanomas and 76 noninvasive melanocytic cutaneous tumors were investigated retrospectively. vV clearly distinguished between noninvasive (average vV = 122 microns 3) and invasive lesions (average vV = 246 microns 3). Most of the patients with malignant melanomas showing an overlap of nuclear vV with benign lesions had a favorable prognosis. No significant differences in vV could be demonstrated among different noninvasive tumor types. Two-dimensional estimates only distinguished benign from malignant tumors with considerable overlap and with significantly varying influence from other factors among different benign lesional types. The variance associated with estimates of vV disclosed a linear relationship to vV, whereas the relative variation seemed to be constant. Tumor heterogeneity was without significant influence on estimates of nuclear vV. It is concluded that nuclear vV is a superior, efficient, and sensitive estimator for distinguishing between melanocytic cutaneous tumors showing different biological behavior, well-suited for objective malignancy grading.",
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Stereological estimation of nuclear volume in benign melanocytic lesions and cutaneous malignant melanomas. / Sørensen, Flemming Brandt.

I: American Journal of Dermatopathology, Bind 11, Nr. 6, 12.1989, s. 517-27.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Stereological estimation of nuclear volume in benign melanocytic lesions and cutaneous malignant melanomas

AU - Sørensen, Flemming Brandt

PY - 1989/12

Y1 - 1989/12

N2 - Recent developments in stereology have made it possible to obtain unbiased, objective estimates of the volume weighted mean nuclear volume (vV) from ordinary histological sections, without any assumptions concerning nuclear shape. The aim of this study was to gather baseline data of nuclear vV in melanocytic cutaneous tumors and to compare these with estimates of nuclear volume fraction and with traditional two-dimensional morphometric estimates of nuclear profile area, nuclear density, and mitotic index. Routinely processed, paraffin embedded tissue specimens from 47 malignant melanomas and 76 noninvasive melanocytic cutaneous tumors were investigated retrospectively. vV clearly distinguished between noninvasive (average vV = 122 microns 3) and invasive lesions (average vV = 246 microns 3). Most of the patients with malignant melanomas showing an overlap of nuclear vV with benign lesions had a favorable prognosis. No significant differences in vV could be demonstrated among different noninvasive tumor types. Two-dimensional estimates only distinguished benign from malignant tumors with considerable overlap and with significantly varying influence from other factors among different benign lesional types. The variance associated with estimates of vV disclosed a linear relationship to vV, whereas the relative variation seemed to be constant. Tumor heterogeneity was without significant influence on estimates of nuclear vV. It is concluded that nuclear vV is a superior, efficient, and sensitive estimator for distinguishing between melanocytic cutaneous tumors showing different biological behavior, well-suited for objective malignancy grading.

AB - Recent developments in stereology have made it possible to obtain unbiased, objective estimates of the volume weighted mean nuclear volume (vV) from ordinary histological sections, without any assumptions concerning nuclear shape. The aim of this study was to gather baseline data of nuclear vV in melanocytic cutaneous tumors and to compare these with estimates of nuclear volume fraction and with traditional two-dimensional morphometric estimates of nuclear profile area, nuclear density, and mitotic index. Routinely processed, paraffin embedded tissue specimens from 47 malignant melanomas and 76 noninvasive melanocytic cutaneous tumors were investigated retrospectively. vV clearly distinguished between noninvasive (average vV = 122 microns 3) and invasive lesions (average vV = 246 microns 3). Most of the patients with malignant melanomas showing an overlap of nuclear vV with benign lesions had a favorable prognosis. No significant differences in vV could be demonstrated among different noninvasive tumor types. Two-dimensional estimates only distinguished benign from malignant tumors with considerable overlap and with significantly varying influence from other factors among different benign lesional types. The variance associated with estimates of vV disclosed a linear relationship to vV, whereas the relative variation seemed to be constant. Tumor heterogeneity was without significant influence on estimates of nuclear vV. It is concluded that nuclear vV is a superior, efficient, and sensitive estimator for distinguishing between melanocytic cutaneous tumors showing different biological behavior, well-suited for objective malignancy grading.

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KW - Adult

KW - Aged

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KW - Humans

KW - Lentigo

KW - Male

KW - Mathematics

KW - Melanocytes

KW - Melanoma

KW - Middle Aged

KW - Nevus

KW - Skin Neoplasms

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