SPR analysis of antibody-antigen interactions using graphene oxide linking layers

Yu V. Stebunov, Aleksey V. Arsenin, Valentyn S. Volkov

Publikation: Bidrag til tidsskriftKonferenceartikelForskningpeer review

Abstrakt

Surface plasmon resonance (SPR) biosensing provides a powerful tool for label-free kinetic analysis of antibody-antigen interactions. SPR analysis can be improved with biosensing interfaces based on graphene oxide (GO), which will enhance the accuracy of determination of kinetic constants and expand a range of suitable targets. GO possesses large surface area and oxygen-containing functional groups for covalent immobilization of biomolecules. Here, we are proposing an SPR biosensing interface based on a GO linking layer with immobilized protein A, which allows orientation-specific immobilization of antibodies. Protein A was covalently attached to the surface of GO via amine coupling. Thereafter, the developed biosensing interface was used for the analysis of the interaction between anti-bovine serum albumin (BSA) antibody and BSA.
OriginalsprogEngelsk
TidsskriftMaterials Today: Proceedings
Vol/bind5
Udgave nummer9 (Part 2)
Sider (fra-til)17442-17446
ISSN2214-7853
DOI
StatusUdgivet - 2018
Begivenhed9th International Conference on Advanced Nano Materials - University of Aveiro, Portugal
Varighed: 19. jul. 201721. jul. 2017

Konference

Konference9th International Conference on Advanced Nano Materials
LokationUniversity of Aveiro
Land/OmrådePortugal
Periode19/07/201721/07/2017

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