Special features of phosphatidylcholine vesicles as seen in cryo-transmission electron microscopy

Beate Klösgen*, Wolfgang Helfrich

*Kontaktforfatter for dette arbejde

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Resumé

Vesicles of egg yolk phosphatidylcholine (EYPC) were studied by cryo-transmission electron microscopy. The electron micrographs indicate that, despite the rapidity of cooling, membrane undulations are flattened and some vesicles change their shapes before the samples freeze. These artefacts are attributed to the action of the lateral tension that results from the membrane area contraction associated with the temperature drop. Other micrographs represent grainy membranes and angular vesicles. We regard them as the first direct evidence for the superstructure and optically invisible roughness which were recently postulated for these membranes.

OriginalsprogEngelsk
TidsskriftEuropean Biophysics Journal
Vol/bind22
Udgave nummer5
Sider (fra-til)329-340
Antal sider12
ISSN0175-7571
DOI
StatusUdgivet - 1. nov. 1993

Fingeraftryk

Cryoelectron Microscopy
Transmission Electron Microscopy
Membranes
Egg Yolk
Artifacts
Electrons

Citer dette

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abstract = "Vesicles of egg yolk phosphatidylcholine (EYPC) were studied by cryo-transmission electron microscopy. The electron micrographs indicate that, despite the rapidity of cooling, membrane undulations are flattened and some vesicles change their shapes before the samples freeze. These artefacts are attributed to the action of the lateral tension that results from the membrane area contraction associated with the temperature drop. Other micrographs represent grainy membranes and angular vesicles. We regard them as the first direct evidence for the superstructure and optically invisible roughness which were recently postulated for these membranes.",
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Special features of phosphatidylcholine vesicles as seen in cryo-transmission electron microscopy. / Klösgen, Beate; Helfrich, Wolfgang.

I: European Biophysics Journal, Bind 22, Nr. 5, 01.11.1993, s. 329-340.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Special features of phosphatidylcholine vesicles as seen in cryo-transmission electron microscopy

AU - Klösgen, Beate

AU - Helfrich, Wolfgang

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N2 - Vesicles of egg yolk phosphatidylcholine (EYPC) were studied by cryo-transmission electron microscopy. The electron micrographs indicate that, despite the rapidity of cooling, membrane undulations are flattened and some vesicles change their shapes before the samples freeze. These artefacts are attributed to the action of the lateral tension that results from the membrane area contraction associated with the temperature drop. Other micrographs represent grainy membranes and angular vesicles. We regard them as the first direct evidence for the superstructure and optically invisible roughness which were recently postulated for these membranes.

AB - Vesicles of egg yolk phosphatidylcholine (EYPC) were studied by cryo-transmission electron microscopy. The electron micrographs indicate that, despite the rapidity of cooling, membrane undulations are flattened and some vesicles change their shapes before the samples freeze. These artefacts are attributed to the action of the lateral tension that results from the membrane area contraction associated with the temperature drop. Other micrographs represent grainy membranes and angular vesicles. We regard them as the first direct evidence for the superstructure and optically invisible roughness which were recently postulated for these membranes.

KW - Cryo-transmission electron microscopy

KW - Membrane roughness

KW - Vesicle shapes

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