Smoking attenuates wound inflammation and proliferation while smoking cessation restores inflammation but not proliferation

Lars Tue Sørensen, Birgitte Grønkær Toft, Jørgen Rygaard, Steen Ladelund, Børge Teisner, Finn Gottrup

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

Full-thickness 5 mm punch biopsy wounds were made lateral to the sacrum in 48 smokers and 30 never smokers. After 1 week, the wounds were excised and fixed. The smokers were then randomized to continuous smoking or abstinence with a transdermal nicotine patch or a placebo patch. The sequence of wounding and excision was repeated after 4, 8, and 12 weeks. All excised tissue was stained with hematoxylin-eosin and immunohistochemically for macrophages (CD68), procollagen 1 N-terminal propeptide (PINP) in fibroblasts, and endothelial cells (CD31). The cellularity was assessed and scored by two independent histopathologists, and for the analysis, proportional odds models and random effect models for repeated measurements were applied. Macrophages and PINP-stained fibroblasts were reduced in the smokers' wounds (0.28 [0.14-0.58] [OR, 95%CI]; p=0.01 and 0.37[0.19-0.70]; p
OriginalsprogEngelsk
TidsskriftWound Repair and Regeneration
Vol/bind18
Udgave nummer2
Sider (fra-til)186-92
Antal sider7
ISSN1067-1927
DOI
StatusUdgivet - 1. mar. 2010

Fingeraftryk

Smoking Cessation
Smoking
Wounds and Injuries
Fibroblasts
Macrophages
Procollagen
Sacrum
Hematoxylin
Eosine Yellowish-(YS)
Placebos

Citer dette

Sørensen, Lars Tue ; Toft, Birgitte Grønkær ; Rygaard, Jørgen ; Ladelund, Steen ; Teisner, Børge ; Gottrup, Finn. / Smoking attenuates wound inflammation and proliferation while smoking cessation restores inflammation but not proliferation. I: Wound Repair and Regeneration. 2010 ; Bind 18, Nr. 2. s. 186-92.
@article{bbe155178c8f4fabae039ffbad27ed00,
title = "Smoking attenuates wound inflammation and proliferation while smoking cessation restores inflammation but not proliferation",
abstract = "Full-thickness 5 mm punch biopsy wounds were made lateral to the sacrum in 48 smokers and 30 never smokers. After 1 week, the wounds were excised and fixed. The smokers were then randomized to continuous smoking or abstinence with a transdermal nicotine patch or a placebo patch. The sequence of wounding and excision was repeated after 4, 8, and 12 weeks. All excised tissue was stained with hematoxylin-eosin and immunohistochemically for macrophages (CD68), procollagen 1 N-terminal propeptide (PINP) in fibroblasts, and endothelial cells (CD31). The cellularity was assessed and scored by two independent histopathologists, and for the analysis, proportional odds models and random effect models for repeated measurements were applied. Macrophages and PINP-stained fibroblasts were reduced in the smokers' wounds (0.28 [0.14-0.58] [OR, 95{\%}CI]; p=0.01 and 0.37[0.19-0.70]; p",
keywords = "Adult, Cell Proliferation, Endothelial Cells, Female, Fibroblasts, Humans, Inflammation, Macrophages, Male, Neovascularization, Physiologic, Peptide Fragments, Procollagen, Skin, Smoking, Smoking Cessation, Wound Healing",
author = "S{\o}rensen, {Lars Tue} and Toft, {Birgitte Gr{\o}nk{\ae}r} and J{\o}rgen Rygaard and Steen Ladelund and B{\o}rge Teisner and Finn Gottrup",
year = "2010",
month = "3",
day = "1",
doi = "10.1111/j.1524-475X.2010.00569.x",
language = "English",
volume = "18",
pages = "186--92",
journal = "Wound Repair and Regeneration",
issn = "1067-1927",
publisher = "Wiley-Blackwell",
number = "2",

}

Smoking attenuates wound inflammation and proliferation while smoking cessation restores inflammation but not proliferation. / Sørensen, Lars Tue; Toft, Birgitte Grønkær; Rygaard, Jørgen; Ladelund, Steen; Teisner, Børge; Gottrup, Finn.

I: Wound Repair and Regeneration, Bind 18, Nr. 2, 01.03.2010, s. 186-92.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Smoking attenuates wound inflammation and proliferation while smoking cessation restores inflammation but not proliferation

AU - Sørensen, Lars Tue

AU - Toft, Birgitte Grønkær

AU - Rygaard, Jørgen

AU - Ladelund, Steen

AU - Teisner, Børge

AU - Gottrup, Finn

PY - 2010/3/1

Y1 - 2010/3/1

N2 - Full-thickness 5 mm punch biopsy wounds were made lateral to the sacrum in 48 smokers and 30 never smokers. After 1 week, the wounds were excised and fixed. The smokers were then randomized to continuous smoking or abstinence with a transdermal nicotine patch or a placebo patch. The sequence of wounding and excision was repeated after 4, 8, and 12 weeks. All excised tissue was stained with hematoxylin-eosin and immunohistochemically for macrophages (CD68), procollagen 1 N-terminal propeptide (PINP) in fibroblasts, and endothelial cells (CD31). The cellularity was assessed and scored by two independent histopathologists, and for the analysis, proportional odds models and random effect models for repeated measurements were applied. Macrophages and PINP-stained fibroblasts were reduced in the smokers' wounds (0.28 [0.14-0.58] [OR, 95%CI]; p=0.01 and 0.37[0.19-0.70]; p

AB - Full-thickness 5 mm punch biopsy wounds were made lateral to the sacrum in 48 smokers and 30 never smokers. After 1 week, the wounds were excised and fixed. The smokers were then randomized to continuous smoking or abstinence with a transdermal nicotine patch or a placebo patch. The sequence of wounding and excision was repeated after 4, 8, and 12 weeks. All excised tissue was stained with hematoxylin-eosin and immunohistochemically for macrophages (CD68), procollagen 1 N-terminal propeptide (PINP) in fibroblasts, and endothelial cells (CD31). The cellularity was assessed and scored by two independent histopathologists, and for the analysis, proportional odds models and random effect models for repeated measurements were applied. Macrophages and PINP-stained fibroblasts were reduced in the smokers' wounds (0.28 [0.14-0.58] [OR, 95%CI]; p=0.01 and 0.37[0.19-0.70]; p

KW - Adult

KW - Cell Proliferation

KW - Endothelial Cells

KW - Female

KW - Fibroblasts

KW - Humans

KW - Inflammation

KW - Macrophages

KW - Male

KW - Neovascularization, Physiologic

KW - Peptide Fragments

KW - Procollagen

KW - Skin

KW - Smoking

KW - Smoking Cessation

KW - Wound Healing

U2 - 10.1111/j.1524-475X.2010.00569.x

DO - 10.1111/j.1524-475X.2010.00569.x

M3 - Journal article

VL - 18

SP - 186

EP - 192

JO - Wound Repair and Regeneration

JF - Wound Repair and Regeneration

SN - 1067-1927

IS - 2

ER -