siRNA nanoparticle functionalization of nanostructured scaffolds enables controlled multilineage differentiation of stem cells

Morten Ø Andersen, Jens Vinge Nygaard, Jorge S Burns, Merete Krog Raarup, Jens Randel Nyengaard, Cody Bünger, Flemming Besenbacher, Kenneth Alan Howard, Moustapha Kassem, Jørgen Kjems

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

The creation of complex tissues and organs is the ultimate goal in tissue engineering. Engineered morphogenesis necessitates spatially controlled development of multiple cell types within a scaffold implant. We present a novel method to achieve this by adhering nanoparticles containing different small-interfering RNAs (siRNAs) into nanostructured scaffolds. This allows spatial retention of the RNAs within nanopores until their cellular delivery. The released siRNAs were capable of gene silencing BCL2L2 and TRIB2, in mesenchymal stem cells (MSCs), enhancing osteogenic and adipogenic differentiation, respectively. This approach for enhancing a single type of differentiation is immediately applicable to all areas of tissue engineering. Different nanoparticles localized to spatially distinct locations within a single implant allowed two different tissue types to develop in controllable areas of an implant. As a consequence of this, we predict that complex tissues and organs can be engineered by the in situ development of multiple cell types guided by spatially restricted nanoparticles.
OriginalsprogEngelsk
TidsskriftMolecular Therapy
Vol/bind18
Udgave nummer11
Sider (fra-til)2018-27
ISSN1525-0016
DOI
StatusUdgivet - 1. nov. 2010

Fingeraftryk

Small Interfering RNA
Tissue Engineering
Mesenchymal Stromal Cells
Morphogenesis
RNA

Citer dette

Andersen, Morten Ø ; Nygaard, Jens Vinge ; Burns, Jorge S ; Raarup, Merete Krog ; Nyengaard, Jens Randel ; Bünger, Cody ; Besenbacher, Flemming ; Howard, Kenneth Alan ; Kassem, Moustapha ; Kjems, Jørgen. / siRNA nanoparticle functionalization of nanostructured scaffolds enables controlled multilineage differentiation of stem cells. I: Molecular Therapy. 2010 ; Bind 18, Nr. 11. s. 2018-27.
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abstract = "The creation of complex tissues and organs is the ultimate goal in tissue engineering. Engineered morphogenesis necessitates spatially controlled development of multiple cell types within a scaffold implant. We present a novel method to achieve this by adhering nanoparticles containing different small-interfering RNAs (siRNAs) into nanostructured scaffolds. This allows spatial retention of the RNAs within nanopores until their cellular delivery. The released siRNAs were capable of gene silencing BCL2L2 and TRIB2, in mesenchymal stem cells (MSCs), enhancing osteogenic and adipogenic differentiation, respectively. This approach for enhancing a single type of differentiation is immediately applicable to all areas of tissue engineering. Different nanoparticles localized to spatially distinct locations within a single implant allowed two different tissue types to develop in controllable areas of an implant. As a consequence of this, we predict that complex tissues and organs can be engineered by the in situ development of multiple cell types guided by spatially restricted nanoparticles.",
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Andersen, MØ, Nygaard, JV, Burns, JS, Raarup, MK, Nyengaard, JR, Bünger, C, Besenbacher, F, Howard, KA, Kassem, M & Kjems, J 2010, 'siRNA nanoparticle functionalization of nanostructured scaffolds enables controlled multilineage differentiation of stem cells', Molecular Therapy, bind 18, nr. 11, s. 2018-27. https://doi.org/10.1038/mt.2010.166

siRNA nanoparticle functionalization of nanostructured scaffolds enables controlled multilineage differentiation of stem cells. / Andersen, Morten Ø; Nygaard, Jens Vinge; Burns, Jorge S; Raarup, Merete Krog; Nyengaard, Jens Randel; Bünger, Cody; Besenbacher, Flemming; Howard, Kenneth Alan; Kassem, Moustapha; Kjems, Jørgen.

I: Molecular Therapy, Bind 18, Nr. 11, 01.11.2010, s. 2018-27.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

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AU - Andersen, Morten Ø

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AU - Burns, Jorge S

AU - Raarup, Merete Krog

AU - Nyengaard, Jens Randel

AU - Bünger, Cody

AU - Besenbacher, Flemming

AU - Howard, Kenneth Alan

AU - Kassem, Moustapha

AU - Kjems, Jørgen

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N2 - The creation of complex tissues and organs is the ultimate goal in tissue engineering. Engineered morphogenesis necessitates spatially controlled development of multiple cell types within a scaffold implant. We present a novel method to achieve this by adhering nanoparticles containing different small-interfering RNAs (siRNAs) into nanostructured scaffolds. This allows spatial retention of the RNAs within nanopores until their cellular delivery. The released siRNAs were capable of gene silencing BCL2L2 and TRIB2, in mesenchymal stem cells (MSCs), enhancing osteogenic and adipogenic differentiation, respectively. This approach for enhancing a single type of differentiation is immediately applicable to all areas of tissue engineering. Different nanoparticles localized to spatially distinct locations within a single implant allowed two different tissue types to develop in controllable areas of an implant. As a consequence of this, we predict that complex tissues and organs can be engineered by the in situ development of multiple cell types guided by spatially restricted nanoparticles.

AB - The creation of complex tissues and organs is the ultimate goal in tissue engineering. Engineered morphogenesis necessitates spatially controlled development of multiple cell types within a scaffold implant. We present a novel method to achieve this by adhering nanoparticles containing different small-interfering RNAs (siRNAs) into nanostructured scaffolds. This allows spatial retention of the RNAs within nanopores until their cellular delivery. The released siRNAs were capable of gene silencing BCL2L2 and TRIB2, in mesenchymal stem cells (MSCs), enhancing osteogenic and adipogenic differentiation, respectively. This approach for enhancing a single type of differentiation is immediately applicable to all areas of tissue engineering. Different nanoparticles localized to spatially distinct locations within a single implant allowed two different tissue types to develop in controllable areas of an implant. As a consequence of this, we predict that complex tissues and organs can be engineered by the in situ development of multiple cell types guided by spatially restricted nanoparticles.

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