Shotgun lipidomics on a LTQ Orbitrap mass spectrometer by successive switching between acquisition polarity modes

K. Schuhmann, R. Almeida, M. Baumert, R. Herzog, S. R. Bornstein, A. Shevchenko

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

Topdown shotgun lipidomics relies on direct infusion of total lipid extracts into a high-resolution tandem mass spectrometer and implies that individual lipids are recognized by their accurately determined m/z. Lipid ionization efficiency and detection specificity strongly depend on the acquisition polarity, and therefore it is beneficial to analyze lipid mixtures in both positive and negative modes. Hybrid LTQ Orbitrap mass spectrometers are widely applied in topdown lipidomics; however, rapid polarity switching was previously unfeasible because of the severe and immediate degradation of mass accuracy. Here, we report on a method to rapidly acquire high-resolution spectra in both polarity modes with sub-ppm mass accuracy and demonstrate that it not only simplifies and accelerates shotgun lipidomics analyses but also improves the lipidome coverage because more lipid classes and more individual species within each class are recognized. In this way, shotgun analysis of total lipid extracts of human blood plasma enabled to quantify 222 species from 15 major lipid classes within 7?min acquisition cycle. Copyright (C) 2012 John Wiley & Sons, Ltd.
OriginalsprogEngelsk
TidsskriftJournal of Mass Spectrometry
Vol/bind47
Udgave nummer1
Sider (fra-til)96-104
Antal sider9
ISSN1076-5174
DOI
StatusUdgivet - 2012

Citer dette

Schuhmann, K. ; Almeida, R. ; Baumert, M. ; Herzog, R. ; Bornstein, S. R. ; Shevchenko, A. / Shotgun lipidomics on a LTQ Orbitrap mass spectrometer by successive switching between acquisition polarity modes. I: Journal of Mass Spectrometry. 2012 ; Bind 47, Nr. 1. s. 96-104.
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Schuhmann, K, Almeida, R, Baumert, M, Herzog, R, Bornstein, SR & Shevchenko, A 2012, 'Shotgun lipidomics on a LTQ Orbitrap mass spectrometer by successive switching between acquisition polarity modes', Journal of Mass Spectrometry, bind 47, nr. 1, s. 96-104. https://doi.org/10.1002/jms.2031

Shotgun lipidomics on a LTQ Orbitrap mass spectrometer by successive switching between acquisition polarity modes. / Schuhmann, K.; Almeida, R.; Baumert, M.; Herzog, R.; Bornstein, S. R.; Shevchenko, A.

I: Journal of Mass Spectrometry, Bind 47, Nr. 1, 2012, s. 96-104.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

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AU - Baumert, M.

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AU - Bornstein, S. R.

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AB - Topdown shotgun lipidomics relies on direct infusion of total lipid extracts into a high-resolution tandem mass spectrometer and implies that individual lipids are recognized by their accurately determined m/z. Lipid ionization efficiency and detection specificity strongly depend on the acquisition polarity, and therefore it is beneficial to analyze lipid mixtures in both positive and negative modes. Hybrid LTQ Orbitrap mass spectrometers are widely applied in topdown lipidomics; however, rapid polarity switching was previously unfeasible because of the severe and immediate degradation of mass accuracy. Here, we report on a method to rapidly acquire high-resolution spectra in both polarity modes with sub-ppm mass accuracy and demonstrate that it not only simplifies and accelerates shotgun lipidomics analyses but also improves the lipidome coverage because more lipid classes and more individual species within each class are recognized. In this way, shotgun analysis of total lipid extracts of human blood plasma enabled to quantify 222 species from 15 major lipid classes within 7?min acquisition cycle. Copyright (C) 2012 John Wiley & Sons, Ltd.

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