Shotgun lipidomics affords comprehensive and quantitative analysis of lipid species in cells and tissues at high-throughput [1 5]. The methodology is based on direct infusion of lipid extracts by electrospray ionization (ESI) combined with tandem mass spectrometry (MS/MS) and/or high resolution Fourier transform mass spectrometry (FTMS) for identification and quantification of lipid species . Shotgun lipidomics affords extensive lipidome coverage by combining the analysis of lipid extracts in positive and negative ion mode [1, 3]. Notably, sterols such as cholesterol and ergosterol exhibit low ionization efficiency in ESI . For this reason, chemical derivatization procedures including acetylation  or sulfation  are commonly implemented to facilitate ionization, detection and quantification of sterols for global lipidome analysis [1-3, 10].