Shotgun lipidomic analysis of chemically sulfated sterols compromises analytical sensitivity: Recommendation for large-scale global lipidome analysis

Albert Casanovas, Hans Kristian Hannibal-Bach, Ole Nørregaard Jensen, Christer S Ejsing

Publikation: Bidrag til tidsskriftKommentar/debatForskningpeer review

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Abstrakt

Shotgun lipidomics affords comprehensive and quantitative analysis of lipid species in cells and tissues at high-throughput [1 5]. The methodology is based on direct infusion of lipid extracts by electrospray ionization (ESI) combined with tandem mass spectrometry (MS/MS) and/or high resolution Fourier transform mass spectrometry (FTMS) for identification and quantification of lipid species [6]. Shotgun lipidomics affords extensive lipidome coverage by combining the analysis of lipid extracts in positive and negative ion mode [1, 3]. Notably, sterols such as cholesterol and ergosterol exhibit low ionization efficiency in ESI [7]. For this reason, chemical derivatization procedures including acetylation [8] or sulfation [9] are commonly implemented to facilitate ionization, detection and quantification of sterols for global lipidome analysis [1-3, 10].
OriginalsprogEngelsk
TidsskriftEuropean Journal of Lipid Science and Technology
Vol/bind116
Udgave nummer12
Sider (fra-til)1618-1620
ISSN1438-7697
DOI
StatusUdgivet - 2014

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