Abstract
Neuropsychiatric systemic lupus erythematosus is an autoimmune disorder characterized by an irregular exchange between the central nervous system and the immune system, leading to the outbreak of neurological conditions with possible disabling effects. Although neuropsychiatric systemic lupus erythematosus is the most common expression of lupus condition, it is still poorly understood. In this study, we focus on the development of an advantageous method based on the application of synthetic nucleic acids and protein-based antigen arrays in order to characterize autoreactive antibodies in neuropsychiatric systemic lupus erythematosus. We confirmed the benefits of using synthetic oligonucleotides such as assay reproducibility, elevated affinity and specificity to autoreactive antibodies. We also demonstrated presence of autoantibodies towards three particular synthetic double stranded antigens and verify similarity of antinuclear antibody patterns in ordinary lupus and neuropsychiatric systemic lupus erythematosus.
| Originalsprog | Engelsk |
|---|---|
| Artikelnummer | 100068 |
| Tidsskrift | Journal of Translational Autoimmunity |
| Vol/bind | 3 |
| Antal sider | 8 |
| DOI | |
| Status | Udgivet - jan. 2020 |
Finansiering
We acknowledge Prof Dr Elizabeth D Mellins and Prof Dr PJ Utz, Stanford University, USA, for providing with SLE control samples. We acknowledge Konsul George Jorck og Hustru Emma Jorck’s Fond research prize, award no. 40934, for supporting the study. The funder had no input into neither the study nor preparing this paper.