Sequence analysis of the Epstein-Barr virus (EBV) latent membrane protein-1 gene and promoter region

identification of four variants among wild-type EBV isolates

K Sandvej, J W Gratama, M Munch, X G Zhou, R L Bolhuis, B S Andresen, N Gregersen, S Hamilton-Dutoit

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

Sequence variations in the Epstein-Barr virus (EBV) encoded latent membrane protein-1 (LMP-1) gene have been described in a Chinese nasopharyngeal carcinoma-derived isolate (CAO), and in viral isolates from various EBV-associated tumors. It has been suggested that these genetic changes, which include loss of a Xho I restriction site (position 169425) and a C-terminal 30-base pair (bp) deletion (position 168287-168256), define EBV genotypes associated with increased tumorigenicity or with disease among particular geographic populations. To determine the frequency of LMP-1 variations in European wild-type virus isolates, we sequenced the LMP-1 promoter and gene in EBV from lymphoblastoid cell lines from healthy carriers and patients without EBV-associated disease. Sequence changes were often present, and defined at least four main groups of viral isolates, which we designate Groups A through D. The widespread prevalence of LMP-1 sequence variations, particularly the Xho I polymorphism and the 30-bp deletion, indicate that they cannot be used as simple markers for oncogenic viruses related to particular forms of EBV-associated tumor. Several of the structural changes detected occur, however, at sites where they may affect transcription, translation, or function of LMP-1. Future in vitro studies should aim to establish the functional importance of variations at these sites.

OriginalsprogEngelsk
TidsskriftBlood
Vol/bind90
Udgave nummer1
Sider (fra-til)323-30
Antal sider8
ISSN0006-4971
StatusUdgivet - 1. jul. 1997

Fingeraftryk

Human Herpesvirus 4
Sequence Analysis
Membrane Proteins
Base Pairing
Oncogenic Viruses
Virus Diseases
Neoplasms
Viruses
Cell Line
Population

Citer dette

Sandvej, K ; Gratama, J W ; Munch, M ; Zhou, X G ; Bolhuis, R L ; Andresen, B S ; Gregersen, N ; Hamilton-Dutoit, S. / Sequence analysis of the Epstein-Barr virus (EBV) latent membrane protein-1 gene and promoter region : identification of four variants among wild-type EBV isolates. I: Blood. 1997 ; Bind 90, Nr. 1. s. 323-30.
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title = "Sequence analysis of the Epstein-Barr virus (EBV) latent membrane protein-1 gene and promoter region: identification of four variants among wild-type EBV isolates",
abstract = "Sequence variations in the Epstein-Barr virus (EBV) encoded latent membrane protein-1 (LMP-1) gene have been described in a Chinese nasopharyngeal carcinoma-derived isolate (CAO), and in viral isolates from various EBV-associated tumors. It has been suggested that these genetic changes, which include loss of a Xho I restriction site (position 169425) and a C-terminal 30-base pair (bp) deletion (position 168287-168256), define EBV genotypes associated with increased tumorigenicity or with disease among particular geographic populations. To determine the frequency of LMP-1 variations in European wild-type virus isolates, we sequenced the LMP-1 promoter and gene in EBV from lymphoblastoid cell lines from healthy carriers and patients without EBV-associated disease. Sequence changes were often present, and defined at least four main groups of viral isolates, which we designate Groups A through D. The widespread prevalence of LMP-1 sequence variations, particularly the Xho I polymorphism and the 30-bp deletion, indicate that they cannot be used as simple markers for oncogenic viruses related to particular forms of EBV-associated tumor. Several of the structural changes detected occur, however, at sites where they may affect transcription, translation, or function of LMP-1. Future in vitro studies should aim to establish the functional importance of variations at these sites.",
keywords = "Amino Acid Sequence, Base Sequence, Cell Line, Genes, Viral, Herpesvirus 4, Human, Humans, Lymphocytes, Molecular Sequence Data, Promoter Regions, Genetic, Sequence Alignment, Sequence Analysis, Viral Matrix Proteins",
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Sandvej, K, Gratama, JW, Munch, M, Zhou, XG, Bolhuis, RL, Andresen, BS, Gregersen, N & Hamilton-Dutoit, S 1997, 'Sequence analysis of the Epstein-Barr virus (EBV) latent membrane protein-1 gene and promoter region: identification of four variants among wild-type EBV isolates', Blood, bind 90, nr. 1, s. 323-30.

Sequence analysis of the Epstein-Barr virus (EBV) latent membrane protein-1 gene and promoter region : identification of four variants among wild-type EBV isolates. / Sandvej, K; Gratama, J W; Munch, M; Zhou, X G; Bolhuis, R L; Andresen, B S; Gregersen, N; Hamilton-Dutoit, S.

I: Blood, Bind 90, Nr. 1, 01.07.1997, s. 323-30.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Sequence analysis of the Epstein-Barr virus (EBV) latent membrane protein-1 gene and promoter region

T2 - identification of four variants among wild-type EBV isolates

AU - Sandvej, K

AU - Gratama, J W

AU - Munch, M

AU - Zhou, X G

AU - Bolhuis, R L

AU - Andresen, B S

AU - Gregersen, N

AU - Hamilton-Dutoit, S

PY - 1997/7/1

Y1 - 1997/7/1

N2 - Sequence variations in the Epstein-Barr virus (EBV) encoded latent membrane protein-1 (LMP-1) gene have been described in a Chinese nasopharyngeal carcinoma-derived isolate (CAO), and in viral isolates from various EBV-associated tumors. It has been suggested that these genetic changes, which include loss of a Xho I restriction site (position 169425) and a C-terminal 30-base pair (bp) deletion (position 168287-168256), define EBV genotypes associated with increased tumorigenicity or with disease among particular geographic populations. To determine the frequency of LMP-1 variations in European wild-type virus isolates, we sequenced the LMP-1 promoter and gene in EBV from lymphoblastoid cell lines from healthy carriers and patients without EBV-associated disease. Sequence changes were often present, and defined at least four main groups of viral isolates, which we designate Groups A through D. The widespread prevalence of LMP-1 sequence variations, particularly the Xho I polymorphism and the 30-bp deletion, indicate that they cannot be used as simple markers for oncogenic viruses related to particular forms of EBV-associated tumor. Several of the structural changes detected occur, however, at sites where they may affect transcription, translation, or function of LMP-1. Future in vitro studies should aim to establish the functional importance of variations at these sites.

AB - Sequence variations in the Epstein-Barr virus (EBV) encoded latent membrane protein-1 (LMP-1) gene have been described in a Chinese nasopharyngeal carcinoma-derived isolate (CAO), and in viral isolates from various EBV-associated tumors. It has been suggested that these genetic changes, which include loss of a Xho I restriction site (position 169425) and a C-terminal 30-base pair (bp) deletion (position 168287-168256), define EBV genotypes associated with increased tumorigenicity or with disease among particular geographic populations. To determine the frequency of LMP-1 variations in European wild-type virus isolates, we sequenced the LMP-1 promoter and gene in EBV from lymphoblastoid cell lines from healthy carriers and patients without EBV-associated disease. Sequence changes were often present, and defined at least four main groups of viral isolates, which we designate Groups A through D. The widespread prevalence of LMP-1 sequence variations, particularly the Xho I polymorphism and the 30-bp deletion, indicate that they cannot be used as simple markers for oncogenic viruses related to particular forms of EBV-associated tumor. Several of the structural changes detected occur, however, at sites where they may affect transcription, translation, or function of LMP-1. Future in vitro studies should aim to establish the functional importance of variations at these sites.

KW - Amino Acid Sequence

KW - Base Sequence

KW - Cell Line

KW - Genes, Viral

KW - Herpesvirus 4, Human

KW - Humans

KW - Lymphocytes

KW - Molecular Sequence Data

KW - Promoter Regions, Genetic

KW - Sequence Alignment

KW - Sequence Analysis

KW - Viral Matrix Proteins

M3 - Journal article

VL - 90

SP - 323

EP - 330

JO - Blood

JF - Blood

SN - 0006-4971

IS - 1

ER -