Self-Assembly of DNA–Peptide Supermolecules: Coiled-Coil Peptide Structures Templated by d-DNA and l-DNA Triplexes Exhibit Chirality-Independent but Orientation-Dependent Stabilizing Cooperativity

Chenguang Lou, Josephine Tuborg Boesen, Niels Johan Christensen, Kasper K. Sørensen, Peter W. Thulstrup, Martin Nors Pedersen, Ernest Giralt, Knud J. Jensen*, Jesper Wengel

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Abstrakt

DNA nanostructures have been designed and used in many different applications. However, the use of nucleic acid scaffolds to promote the self-assembly of artificial protein mimics is only starting to emerge. Herein five coiled-coil peptide structures were templated by the hybridization of a d-DNA triplex or its mirror-image counterpart, an l-DNA triplex. The self-assembly of the desired trimeric structures in solution was confirmed by gel electrophoresis and small-angle X-ray scattering, and the stabilizing synergy between the two domains was found to be chirality-independent but orientation-dependent. This is the first example of using a nucleic acid scaffold of l-DNA to template the formation of artificial protein mimics. The results may advance the emerging POC-based nanotechnology field by adding two extra dimensions, that is, chirality and polarity, to provide innovative molecular tools for rational design and bottom-up construction of artificial protein mimics, programmable materials and responsive nanodevices.
OriginalsprogEngelsk
TidsskriftChemistry - A European Journal
Vol/bind26
Udgave nummer25
Sider (fra-til)5676-5684
ISSN0947-6539
DOI
StatusUdgivet - 4. apr. 2020

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