Self-assembled composite matrix in a hierarchical 3D scaffold for bone tissue engineering

M Chen, D Q S Le, Anette Baatrup, J V Nygaard, S Hein, L Bjerre, M Kassem, Xuenong Zou, Cody Bünger

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

It is of high clinical relevance in bone tissue engineering that scaffolds promote a high seeding efficiency of cells capable of osteogenic differentiation, such as human bone marrow-derived mesenchymal stem cells (hMSC). We evaluated the effects of a novel polycaprolactone (PCL) scaffold on hMSC seeding efficiency, proliferation, distribution, and differentiation. Porous PCL meshes prepared by fused deposition modeling (FDM) were embedded in matrix of hyaluronic acid, methylated collagen, and terpolymer via polyelectrolyte complex coacervation. Scaffolds were cultured statically and dynamically in osteogenic stimulation medium for up to 28 days. Compared to naked PCL scaffolds, embedded scaffolds provided a higher cell seeding efficiency (t-test, p
OriginalsprogEngelsk
TidsskriftActa Biomaterialia
Vol/bind7
Udgave nummer5
Sider (fra-til)2244-2255
Antal sider12
ISSN1742-7061
DOI
StatusUdgivet - 2011

Fingeraftryk

Polycaprolactone
Tissue Engineering
Scaffolds (biology)
Tissue engineering
Scaffolds
Bone
Composite materials
Hyaluronic acid
Terpolymers
Hyaluronic Acid
Tissue Scaffolds
Stem cells
Polyelectrolytes
Mesenchymal Stromal Cells
Collagen
polycaprolactone

Citer dette

Chen, M., Le, D. Q. S., Baatrup, A., Nygaard, J. V., Hein, S., Bjerre, L., ... Bünger, C. (2011). Self-assembled composite matrix in a hierarchical 3D scaffold for bone tissue engineering. Acta Biomaterialia, 7(5), 2244-2255. https://doi.org/10.1016/j.actbio.2010.12.031
Chen, M ; Le, D Q S ; Baatrup, Anette ; Nygaard, J V ; Hein, S ; Bjerre, L ; Kassem, M ; Zou, Xuenong ; Bünger, Cody. / Self-assembled composite matrix in a hierarchical 3D scaffold for bone tissue engineering. I: Acta Biomaterialia. 2011 ; Bind 7, Nr. 5. s. 2244-2255.
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Chen, M, Le, DQS, Baatrup, A, Nygaard, JV, Hein, S, Bjerre, L, Kassem, M, Zou, X & Bünger, C 2011, 'Self-assembled composite matrix in a hierarchical 3D scaffold for bone tissue engineering', Acta Biomaterialia, bind 7, nr. 5, s. 2244-2255. https://doi.org/10.1016/j.actbio.2010.12.031

Self-assembled composite matrix in a hierarchical 3D scaffold for bone tissue engineering. / Chen, M; Le, D Q S; Baatrup, Anette; Nygaard, J V; Hein, S; Bjerre, L; Kassem, M; Zou, Xuenong; Bünger, Cody.

I: Acta Biomaterialia, Bind 7, Nr. 5, 2011, s. 2244-2255.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Self-assembled composite matrix in a hierarchical 3D scaffold for bone tissue engineering

AU - Chen, M

AU - Le, D Q S

AU - Baatrup, Anette

AU - Nygaard, J V

AU - Hein, S

AU - Bjerre, L

AU - Kassem, M

AU - Zou, Xuenong

AU - Bünger, Cody

N1 - Copyright © 2010. Published by Elsevier Ltd.

PY - 2011

Y1 - 2011

N2 - It is of high clinical relevance in bone tissue engineering that scaffolds promote a high seeding efficiency of cells capable of osteogenic differentiation, such as human bone marrow-derived mesenchymal stem cells (hMSC). We evaluated the effects of a novel polycaprolactone (PCL) scaffold on hMSC seeding efficiency, proliferation, distribution, and differentiation. Porous PCL meshes prepared by fused deposition modeling (FDM) were embedded in matrix of hyaluronic acid, methylated collagen, and terpolymer via polyelectrolyte complex coacervation. Scaffolds were cultured statically and dynamically in osteogenic stimulation medium for up to 28 days. Compared to naked PCL scaffolds, embedded scaffolds provided a higher cell seeding efficiency (t-test, p

AB - It is of high clinical relevance in bone tissue engineering that scaffolds promote a high seeding efficiency of cells capable of osteogenic differentiation, such as human bone marrow-derived mesenchymal stem cells (hMSC). We evaluated the effects of a novel polycaprolactone (PCL) scaffold on hMSC seeding efficiency, proliferation, distribution, and differentiation. Porous PCL meshes prepared by fused deposition modeling (FDM) were embedded in matrix of hyaluronic acid, methylated collagen, and terpolymer via polyelectrolyte complex coacervation. Scaffolds were cultured statically and dynamically in osteogenic stimulation medium for up to 28 days. Compared to naked PCL scaffolds, embedded scaffolds provided a higher cell seeding efficiency (t-test, p

U2 - 10.1016/j.actbio.2010.12.031

DO - 10.1016/j.actbio.2010.12.031

M3 - Journal article

C2 - 21195810

VL - 7

SP - 2244

EP - 2255

JO - Acta Biomaterialia

JF - Acta Biomaterialia

SN - 1742-7061

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