Secondary structure of prokaryotic 5S ribosomal ribonucleic acids: a study with ribonucleases

S Douthwaite, R A Garrett

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

Udgivelsesdato: 1981-Dec-8
OriginalsprogEngelsk
TidsskriftBiochemistry
Vol/bind20
Udgave nummer25
Sider (fra-til)7301-7307
Antal sider6
ISSN0006-2960
StatusUdgivet - 1981

Fingeraftryk

Ribonucleases
ribonuclease T(2)
RNA
5S Ribosomal RNA
Ribonuclease T1
Cobra Venoms
Pancreatic Ribonuclease
Bacilli
Labeling
Escherichia coli
Nucleotides
Molecules
Proteins

Citer dette

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title = "Secondary structure of prokaryotic 5S ribosomal ribonucleic acids: a study with ribonucleases",
abstract = "The structures of 5S ribosomal RNAs from Escherichia coli and Bacillus stearothermophilus were examined by using ribonucleases A, T1, and T2 and a double helix specific cobra venom ribonuclease. By using both 5' and 3'-32P-end labeling methods and selecting for digested but intact 5S RNA molecules, we were able to distinguish between primary and secondary cutting positions and also to establish the relative degree of cutting. The data reveal the predicted similarities of the higher order structure in the two RNAs but also demonstrate a few significant differences. The data also provide direct evidence for three of the helical regions of the Fox and Woese model of 5S RNA [Fox, G. E., & Woese, C. (1975) Nature (London) 256, 505] and support other important structural features which include a nucleotide looped out from a helical region which has been proposed as a recognition site for protein L18.",
keywords = "Bacillus stearothermophilus, Base Sequence, Endonucleases, Endoribonucleases, Escherichia coli, Nucleic Acid Conformation, RNA, Ribosomal, Ribonuclease T1, Ribonuclease, Pancreatic, Ribonucleases",
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journal = "Biochemistry",
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Secondary structure of prokaryotic 5S ribosomal ribonucleic acids: a study with ribonucleases. / Douthwaite, S; Garrett, R A.

I: Biochemistry, Bind 20, Nr. 25, 1981, s. 7301-7307.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Secondary structure of prokaryotic 5S ribosomal ribonucleic acids: a study with ribonucleases

AU - Douthwaite, S

AU - Garrett, R A

PY - 1981

Y1 - 1981

N2 - The structures of 5S ribosomal RNAs from Escherichia coli and Bacillus stearothermophilus were examined by using ribonucleases A, T1, and T2 and a double helix specific cobra venom ribonuclease. By using both 5' and 3'-32P-end labeling methods and selecting for digested but intact 5S RNA molecules, we were able to distinguish between primary and secondary cutting positions and also to establish the relative degree of cutting. The data reveal the predicted similarities of the higher order structure in the two RNAs but also demonstrate a few significant differences. The data also provide direct evidence for three of the helical regions of the Fox and Woese model of 5S RNA [Fox, G. E., & Woese, C. (1975) Nature (London) 256, 505] and support other important structural features which include a nucleotide looped out from a helical region which has been proposed as a recognition site for protein L18.

AB - The structures of 5S ribosomal RNAs from Escherichia coli and Bacillus stearothermophilus were examined by using ribonucleases A, T1, and T2 and a double helix specific cobra venom ribonuclease. By using both 5' and 3'-32P-end labeling methods and selecting for digested but intact 5S RNA molecules, we were able to distinguish between primary and secondary cutting positions and also to establish the relative degree of cutting. The data reveal the predicted similarities of the higher order structure in the two RNAs but also demonstrate a few significant differences. The data also provide direct evidence for three of the helical regions of the Fox and Woese model of 5S RNA [Fox, G. E., & Woese, C. (1975) Nature (London) 256, 505] and support other important structural features which include a nucleotide looped out from a helical region which has been proposed as a recognition site for protein L18.

KW - Bacillus stearothermophilus

KW - Base Sequence

KW - Endonucleases

KW - Endoribonucleases

KW - Escherichia coli

KW - Nucleic Acid Conformation

KW - RNA, Ribosomal

KW - Ribonuclease T1

KW - Ribonuclease, Pancreatic

KW - Ribonucleases

M3 - Journal article

VL - 20

SP - 7301

EP - 7307

JO - Biochemistry

JF - Biochemistry

SN - 0006-2960

IS - 25

ER -