Screening for bioactive metabolites in plant extracts modulating glucose uptake and fat accumulation

Rime B El-Houri, Dorota Kotowska, Louise C B Olsen, Sumangala Bhattacharya, Lars P Christensen, Kai Grevsen, Niels Oksbjerg, Nils J. Færgeman, Karsten Kristiansen, Kathrine B Christensen

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

Dichloromethane and methanol extracts of seven different food and medicinal plants were tested in a screening platform for identification of extracts with potential bioactivity related to insulin-dependent glucose uptake and fat accumulation. The screening platform included a series of in vitro bioassays, peroxisome proliferator-activated receptor (PPAR) γ-mediated transactivation, adipocyte differentiation of 3T3-L1 cell cultures, and glucose uptake in both 3T3-L1 adipocytes and primary porcine myotubes, as well as one in vivo bioassay, fat accumulation in the nematode Caenorhabditis elegans. We found that dichloromethane extracts of aerial parts of golden root (Rhodiola rosea) and common elder (Sambucus nigra) as well as the dichloromethane extracts of thyme (Thymus vulgaris) and carrot (Daucus carota) were able to stimulate insulin-dependent glucose uptake in both adipocytes and myotubes while weekly activating PPARγ without promoting adipocyte differentiation. In addition, these extracts were able to decrease fat accumulation in C. elegans. Methanol extracts of summer savory (Satureja hortensis), common elder, and broccoli (Brassica oleracea) enhanced glucose uptake in myotubes but were not able to activate PPARγ, indicating a PPARγ-independent effect on glucose uptake.

OriginalsprogEngelsk
ArtikelnummerArticle ID 156398
TidsskriftEvidence-Based Complementary and Alternative Medicine
Vol/bind2014
Sider (fra-til)156398
Antal sider8
ISSN1741-427X
DOI
StatusUdgivet - 2014

Fingeraftryk

Adipocytes
Fats
Methylene Chloride
Satureja
Daucus carota
Caenorhabditis elegans
Methanol
Sambucus nigra
Insulin
3T3-L1 Cells
Edible Plants
Medicinal Plants
Transcriptional Activation

Citer dette

El-Houri, Rime B ; Kotowska, Dorota ; Olsen, Louise C B ; Bhattacharya, Sumangala ; Christensen, Lars P ; Grevsen, Kai ; Oksbjerg, Niels ; Færgeman, Nils J. ; Kristiansen, Karsten ; Christensen, Kathrine B. / Screening for bioactive metabolites in plant extracts modulating glucose uptake and fat accumulation. I: Evidence-Based Complementary and Alternative Medicine. 2014 ; Bind 2014. s. 156398.
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abstract = "Dichloromethane and methanol extracts of seven different food and medicinal plants were tested in a screening platform for identification of extracts with potential bioactivity related to insulin-dependent glucose uptake and fat accumulation. The screening platform included a series of in vitro bioassays, peroxisome proliferator-activated receptor (PPAR) γ-mediated transactivation, adipocyte differentiation of 3T3-L1 cell cultures, and glucose uptake in both 3T3-L1 adipocytes and primary porcine myotubes, as well as one in vivo bioassay, fat accumulation in the nematode Caenorhabditis elegans. We found that dichloromethane extracts of aerial parts of golden root (Rhodiola rosea) and common elder (Sambucus nigra) as well as the dichloromethane extracts of thyme (Thymus vulgaris) and carrot (Daucus carota) were able to stimulate insulin-dependent glucose uptake in both adipocytes and myotubes while weekly activating PPARγ without promoting adipocyte differentiation. In addition, these extracts were able to decrease fat accumulation in C. elegans. Methanol extracts of summer savory (Satureja hortensis), common elder, and broccoli (Brassica oleracea) enhanced glucose uptake in myotubes but were not able to activate PPARγ, indicating a PPARγ-independent effect on glucose uptake.",
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El-Houri, RB, Kotowska, D, Olsen, LCB, Bhattacharya, S, Christensen, LP, Grevsen, K, Oksbjerg, N, Færgeman, NJ, Kristiansen, K & Christensen, KB 2014, 'Screening for bioactive metabolites in plant extracts modulating glucose uptake and fat accumulation', Evidence-Based Complementary and Alternative Medicine, bind 2014, Article ID 156398, s. 156398. https://doi.org/10.1155/2014/156398

Screening for bioactive metabolites in plant extracts modulating glucose uptake and fat accumulation. / El-Houri, Rime B; Kotowska, Dorota; Olsen, Louise C B; Bhattacharya, Sumangala; Christensen, Lars P; Grevsen, Kai; Oksbjerg, Niels; Færgeman, Nils J.; Kristiansen, Karsten; Christensen, Kathrine B.

I: Evidence-Based Complementary and Alternative Medicine, Bind 2014, Article ID 156398, 2014, s. 156398.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Screening for bioactive metabolites in plant extracts modulating glucose uptake and fat accumulation

AU - El-Houri, Rime B

AU - Kotowska, Dorota

AU - Olsen, Louise C B

AU - Bhattacharya, Sumangala

AU - Christensen, Lars P

AU - Grevsen, Kai

AU - Oksbjerg, Niels

AU - Færgeman, Nils J.

AU - Kristiansen, Karsten

AU - Christensen, Kathrine B

PY - 2014

Y1 - 2014

N2 - Dichloromethane and methanol extracts of seven different food and medicinal plants were tested in a screening platform for identification of extracts with potential bioactivity related to insulin-dependent glucose uptake and fat accumulation. The screening platform included a series of in vitro bioassays, peroxisome proliferator-activated receptor (PPAR) γ-mediated transactivation, adipocyte differentiation of 3T3-L1 cell cultures, and glucose uptake in both 3T3-L1 adipocytes and primary porcine myotubes, as well as one in vivo bioassay, fat accumulation in the nematode Caenorhabditis elegans. We found that dichloromethane extracts of aerial parts of golden root (Rhodiola rosea) and common elder (Sambucus nigra) as well as the dichloromethane extracts of thyme (Thymus vulgaris) and carrot (Daucus carota) were able to stimulate insulin-dependent glucose uptake in both adipocytes and myotubes while weekly activating PPARγ without promoting adipocyte differentiation. In addition, these extracts were able to decrease fat accumulation in C. elegans. Methanol extracts of summer savory (Satureja hortensis), common elder, and broccoli (Brassica oleracea) enhanced glucose uptake in myotubes but were not able to activate PPARγ, indicating a PPARγ-independent effect on glucose uptake.

AB - Dichloromethane and methanol extracts of seven different food and medicinal plants were tested in a screening platform for identification of extracts with potential bioactivity related to insulin-dependent glucose uptake and fat accumulation. The screening platform included a series of in vitro bioassays, peroxisome proliferator-activated receptor (PPAR) γ-mediated transactivation, adipocyte differentiation of 3T3-L1 cell cultures, and glucose uptake in both 3T3-L1 adipocytes and primary porcine myotubes, as well as one in vivo bioassay, fat accumulation in the nematode Caenorhabditis elegans. We found that dichloromethane extracts of aerial parts of golden root (Rhodiola rosea) and common elder (Sambucus nigra) as well as the dichloromethane extracts of thyme (Thymus vulgaris) and carrot (Daucus carota) were able to stimulate insulin-dependent glucose uptake in both adipocytes and myotubes while weekly activating PPARγ without promoting adipocyte differentiation. In addition, these extracts were able to decrease fat accumulation in C. elegans. Methanol extracts of summer savory (Satureja hortensis), common elder, and broccoli (Brassica oleracea) enhanced glucose uptake in myotubes but were not able to activate PPARγ, indicating a PPARγ-independent effect on glucose uptake.

U2 - 10.1155/2014/156398

DO - 10.1155/2014/156398

M3 - Journal article

VL - 2014

SP - 156398

JO - Evidence-Based Complementary and Alternative Medicine

JF - Evidence-Based Complementary and Alternative Medicine

SN - 1741-427X

M1 - Article ID 156398

ER -