TY - JOUR
T1 - Scoring the tumor-stroma ratio in colon cancer
T2 - procedure and recommendations
AU - van Pelt, G. W.
AU - Kjær-Frifeldt, S.
AU - van Krieken, J. H.J.M.
AU - Al Dieri, R.
AU - Morreau, H.
AU - Tollenaar, R. A.E.M.
AU - Sørensen, F. B.
AU - Mesker, W. E.
PY - 2018/10/1
Y1 - 2018/10/1
N2 - The tumor-stroma ratio (TSR) has been reported as a strong, independent prognostic parameter in colon cancer as well as in other epithelial cancer types, and may be implemented to routine pathology diagnostics. The TSR is an easy technique, based on routine hematoxylin and eosin stained histological sections, estimating the amount of stroma present in the primary tumor. It links tumors with high stromal content to poor prognosis. The analysis time is less than 2 min with a low inter-observer variation. Scoring of the TSR has been validated in a number of independent international studies. In this manuscript, we provide a detailed technical description of estimating the TSR in colon cancer, including examples, pitfalls, and recommendations.
AB - The tumor-stroma ratio (TSR) has been reported as a strong, independent prognostic parameter in colon cancer as well as in other epithelial cancer types, and may be implemented to routine pathology diagnostics. The TSR is an easy technique, based on routine hematoxylin and eosin stained histological sections, estimating the amount of stroma present in the primary tumor. It links tumors with high stromal content to poor prognosis. The analysis time is less than 2 min with a low inter-observer variation. Scoring of the TSR has been validated in a number of independent international studies. In this manuscript, we provide a detailed technical description of estimating the TSR in colon cancer, including examples, pitfalls, and recommendations.
KW - Colonic Neoplasms/pathology
KW - Coloring Agents
KW - Eosine Yellowish-(YS)
KW - Epithelial Cells/pathology
KW - Hematoxylin
KW - Humans
KW - Microscopy
KW - Observer Variation
KW - Predictive Value of Tests
KW - Prognosis
KW - Reproducibility of Results
KW - Staining and Labeling/methods
KW - Stromal Cells/pathology
KW - Workflow
U2 - 10.1007/s00428-018-2408-z
DO - 10.1007/s00428-018-2408-z
M3 - Journal article
C2 - 30030621
AN - SCOPUS:85050364831
SN - 0945-6317
VL - 473
SP - 405
EP - 412
JO - Virchows Archiv
JF - Virchows Archiv
IS - 4
ER -