SCE nordic αamylase study II: Assessment of proposed calibration procedure. A report by the scandinavian committee on enzymes (SCE)

W. Gerhardt*, J. Waldenström, M. Hörder, E. Magid, J. H. Strömme, L. Theodorsen, M. Harkönen, A. Icén, P. Hyltoft Petersen, P. J. Jörgensen

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Abstrakt

Eighty-seven Nordic Hospital laboratories participated in a joint SCE-NORDKEM follow-up study of the long-term stability of the previously established calibration factors for a number of αamylase routine methods based on six different substrates. Human control materials with 90% pancreatic, 90% salivary, and pure pancreatic αamylases were measured by the participants. The data were plotted before and after calibration of each method using a human pancreatic calibrator with an assigned catalytic concentration of 390 U/l (Phadebas® blue starch method, 37 °C). As in the previous study, carried out 9 months earlier, the pre-calibration values varied over a six-fold range. The post-calibration values of all methods except those based on a tetraose substrate showed an acceptable inter-laboratory comparability. As a temporary measure, SCE recommends that the Nordic laboratories calibrate the accepted routine methods by their individual calibration factor. Detailed suggestions for calibration procedures and a discussion of the principles of transferability will shortly be published by the SCE in this journal.

OriginalsprogEngelsk
BogserieScandinavian Journal of Clinical and Laboratory Investigation
Vol/bind46
Udgave nummer5
Sider (fra-til)465-469
Antal sider5
ISSN0036-5513
DOI
StatusUdgivet - 1. jan. 1986

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