Routine noninvasive prenatal screening for fetal RHD in plasma of RhD-negative pregnant women-2 years of screening experience from Denmark

F Banch Clausen, R Steffensen, M Christiansen, M Rudby, M A Jakobsen, T R Jakobsen, G R Krog, R D Madsen, K R Nielsen, K Rieneck, U Sprogøe, K M Homburg, J Baech, M H Dziegiel, N Grunnet

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

OBJECTIVE: Prenatal and postnatal RhD prophylaxis reduces the risk of RhD immunization in pregnancies of RhD-negative women. Based on the result from prenatal screening for the fetal RHD gene, prenatal RhD prophylaxis in Denmark is targeted to RhD-negative women who carry an RhD-positive fetus. Here, we present a 2-year evaluation of a nationwide prenatal RHD screening.

METHODS: Blood samples were drawn from RhD-negative women in gestational week 25. DNA was extracted from maternal plasma and analyzed for the RHD gene. The prenatal RHD results were compared with the serological typing of newborns in 12,668 pregnancies. Early compliance was assessed for 690 pregnancies.

RESULTS: The sensitivity for the detection of fetal RHD was 99.9% (95% CI: 99.7-99.9%). Unnecessary recommendation of prenatal RhD prophylaxis was avoided in 97.3% of the women carrying an RhD-negative fetus. Fetuses that were seropositive for RhD were not detected in 11 pregnancies (0.087%). The sample uptake percentage was 84.2%, and the compliance for prenatal anti-D administration was 93.2%.

CONCLUSION: The high sensitivity, maintained over 2 years, underlines the reliability of routine prenatal fetal RHD screening in RhD-negative pregnant women, specifically at 25 weeks of gestation. The remaining challenges are logistical and are related to program compliance.

OriginalsprogEngelsk
TidsskriftPrenatal Diagnosis
Vol/bind34
Udgave nummer10
Sider (fra-til)1000-5
Antal sider6
ISSN0197-3851
DOI
StatusUdgivet - 2014

Fingeraftryk

Denmark
Prenatal Diagnosis
Pregnant Women
Compliance
Fetus
Mothers
Newborn Infant
DNA

Citer dette

Banch Clausen, F ; Steffensen, R ; Christiansen, M ; Rudby, M ; Jakobsen, M A ; Jakobsen, T R ; Krog, G R ; Madsen, R D ; Nielsen, K R ; Rieneck, K ; Sprogøe, U ; Homburg, K M ; Baech, J ; Dziegiel, M H ; Grunnet, N. / Routine noninvasive prenatal screening for fetal RHD in plasma of RhD-negative pregnant women-2 years of screening experience from Denmark. I: Prenatal Diagnosis. 2014 ; Bind 34, Nr. 10. s. 1000-5.
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title = "Routine noninvasive prenatal screening for fetal RHD in plasma of RhD-negative pregnant women-2 years of screening experience from Denmark",
abstract = "OBJECTIVE: Prenatal and postnatal RhD prophylaxis reduces the risk of RhD immunization in pregnancies of RhD-negative women. Based on the result from prenatal screening for the fetal RHD gene, prenatal RhD prophylaxis in Denmark is targeted to RhD-negative women who carry an RhD-positive fetus. Here, we present a 2-year evaluation of a nationwide prenatal RHD screening.METHODS: Blood samples were drawn from RhD-negative women in gestational week 25. DNA was extracted from maternal plasma and analyzed for the RHD gene. The prenatal RHD results were compared with the serological typing of newborns in 12,668 pregnancies. Early compliance was assessed for 690 pregnancies.RESULTS: The sensitivity for the detection of fetal RHD was 99.9{\%} (95{\%} CI: 99.7-99.9{\%}). Unnecessary recommendation of prenatal RhD prophylaxis was avoided in 97.3{\%} of the women carrying an RhD-negative fetus. Fetuses that were seropositive for RhD were not detected in 11 pregnancies (0.087{\%}). The sample uptake percentage was 84.2{\%}, and the compliance for prenatal anti-D administration was 93.2{\%}.CONCLUSION: The high sensitivity, maintained over 2 years, underlines the reliability of routine prenatal fetal RHD screening in RhD-negative pregnant women, specifically at 25 weeks of gestation. The remaining challenges are logistical and are related to program compliance.",
author = "{Banch Clausen}, F and R Steffensen and M Christiansen and M Rudby and Jakobsen, {M A} and Jakobsen, {T R} and Krog, {G R} and Madsen, {R D} and Nielsen, {K R} and K Rieneck and U Sprog{\o}e and Homburg, {K M} and J Baech and Dziegiel, {M H} and N Grunnet",
note = "{\circledC} 2014 John Wiley & Sons, Ltd.",
year = "2014",
doi = "10.1002/pd.4419",
language = "English",
volume = "34",
pages = "1000--5",
journal = "Prenatal Diagnosis",
issn = "0197-3851",
publisher = "JohnWiley & Sons Ltd.",
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Banch Clausen, F, Steffensen, R, Christiansen, M, Rudby, M, Jakobsen, MA, Jakobsen, TR, Krog, GR, Madsen, RD, Nielsen, KR, Rieneck, K, Sprogøe, U, Homburg, KM, Baech, J, Dziegiel, MH & Grunnet, N 2014, 'Routine noninvasive prenatal screening for fetal RHD in plasma of RhD-negative pregnant women-2 years of screening experience from Denmark', Prenatal Diagnosis, bind 34, nr. 10, s. 1000-5. https://doi.org/10.1002/pd.4419

Routine noninvasive prenatal screening for fetal RHD in plasma of RhD-negative pregnant women-2 years of screening experience from Denmark. / Banch Clausen, F; Steffensen, R; Christiansen, M; Rudby, M; Jakobsen, M A; Jakobsen, T R; Krog, G R; Madsen, R D; Nielsen, K R; Rieneck, K; Sprogøe, U; Homburg, K M; Baech, J; Dziegiel, M H; Grunnet, N.

I: Prenatal Diagnosis, Bind 34, Nr. 10, 2014, s. 1000-5.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Routine noninvasive prenatal screening for fetal RHD in plasma of RhD-negative pregnant women-2 years of screening experience from Denmark

AU - Banch Clausen, F

AU - Steffensen, R

AU - Christiansen, M

AU - Rudby, M

AU - Jakobsen, M A

AU - Jakobsen, T R

AU - Krog, G R

AU - Madsen, R D

AU - Nielsen, K R

AU - Rieneck, K

AU - Sprogøe, U

AU - Homburg, K M

AU - Baech, J

AU - Dziegiel, M H

AU - Grunnet, N

N1 - © 2014 John Wiley & Sons, Ltd.

PY - 2014

Y1 - 2014

N2 - OBJECTIVE: Prenatal and postnatal RhD prophylaxis reduces the risk of RhD immunization in pregnancies of RhD-negative women. Based on the result from prenatal screening for the fetal RHD gene, prenatal RhD prophylaxis in Denmark is targeted to RhD-negative women who carry an RhD-positive fetus. Here, we present a 2-year evaluation of a nationwide prenatal RHD screening.METHODS: Blood samples were drawn from RhD-negative women in gestational week 25. DNA was extracted from maternal plasma and analyzed for the RHD gene. The prenatal RHD results were compared with the serological typing of newborns in 12,668 pregnancies. Early compliance was assessed for 690 pregnancies.RESULTS: The sensitivity for the detection of fetal RHD was 99.9% (95% CI: 99.7-99.9%). Unnecessary recommendation of prenatal RhD prophylaxis was avoided in 97.3% of the women carrying an RhD-negative fetus. Fetuses that were seropositive for RhD were not detected in 11 pregnancies (0.087%). The sample uptake percentage was 84.2%, and the compliance for prenatal anti-D administration was 93.2%.CONCLUSION: The high sensitivity, maintained over 2 years, underlines the reliability of routine prenatal fetal RHD screening in RhD-negative pregnant women, specifically at 25 weeks of gestation. The remaining challenges are logistical and are related to program compliance.

AB - OBJECTIVE: Prenatal and postnatal RhD prophylaxis reduces the risk of RhD immunization in pregnancies of RhD-negative women. Based on the result from prenatal screening for the fetal RHD gene, prenatal RhD prophylaxis in Denmark is targeted to RhD-negative women who carry an RhD-positive fetus. Here, we present a 2-year evaluation of a nationwide prenatal RHD screening.METHODS: Blood samples were drawn from RhD-negative women in gestational week 25. DNA was extracted from maternal plasma and analyzed for the RHD gene. The prenatal RHD results were compared with the serological typing of newborns in 12,668 pregnancies. Early compliance was assessed for 690 pregnancies.RESULTS: The sensitivity for the detection of fetal RHD was 99.9% (95% CI: 99.7-99.9%). Unnecessary recommendation of prenatal RhD prophylaxis was avoided in 97.3% of the women carrying an RhD-negative fetus. Fetuses that were seropositive for RhD were not detected in 11 pregnancies (0.087%). The sample uptake percentage was 84.2%, and the compliance for prenatal anti-D administration was 93.2%.CONCLUSION: The high sensitivity, maintained over 2 years, underlines the reliability of routine prenatal fetal RHD screening in RhD-negative pregnant women, specifically at 25 weeks of gestation. The remaining challenges are logistical and are related to program compliance.

U2 - 10.1002/pd.4419

DO - 10.1002/pd.4419

M3 - Journal article

VL - 34

SP - 1000

EP - 1005

JO - Prenatal Diagnosis

JF - Prenatal Diagnosis

SN - 0197-3851

IS - 10

ER -