Role of PPAR-gamma in the modulation of CD36 and FcgammaRII induced by LDL with low and high degrees of oxidation during the differentiation of the monocytic THP-1 cell line

Francisco J O Rios, Sonia Jancar, Ivo B Melo, Daniel F J Ketelhuth, Magnus Gidlund

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

Scavenger or Fcgamma receptors are important for capture and clearance of modified LDL particles by monocytes/macrophages. Uptake via scavenger receptors is not regulated by intracellular levels of cholesterol and in consequence, macrophages develop into foam cells in the arterial intima. The levels of scavenger receptor CD36 are increased in atherosclerotic lesions and there is evidence that some components of oxLDL auto-regulate the expression of this receptor. Fcgamma receptor expression is increased in cardiovascular diseases but it is not known weather their expression is regulated by oxLDL. The biological properties of oxLDLs vary depending on the degree of oxidation. In the present study we investigated the effect of LDL particles showing extensive or low oxidation (HoxLDL and LoxLDL) on the expression of CD36 and FcgammaRII in a human monocytic cell line (THP-1), differentiated or not to macrophage, and the involvement of PPARgamma. It was found that both forms of oxLDL are able to increase the expression of CD36 and FcgammaRII and that this effect is dependent on the degree of oxidation and of the stage of cell differentiation (monocyte or macrophage). We also showed that the increased expression of FcgammaRII is dependent on PPARgamma whereas that of the CD36 is independent of PPARgamma.

OriginalsprogEngelsk
TidsskriftCellular Physiology and Biochemistry
Vol/bind22
Udgave nummer5-6
Sider (fra-til)549-56
Antal sider8
ISSN1015-8987
DOI
StatusUdgivet - 2008

Fingeraftryk

PPAR gamma
Cell Line
Macrophages
Scavenger Receptors
Foam Cells
Cell Differentiation
oxidized low density lipoprotein

Bibliografisk note

Copyright 2008 S. Karger AG, Basel.

Citer dette

@article{ecfdd44e88954c74825cd3e50ec4ef30,
title = "Role of PPAR-gamma in the modulation of CD36 and FcgammaRII induced by LDL with low and high degrees of oxidation during the differentiation of the monocytic THP-1 cell line",
abstract = "Scavenger or Fcgamma receptors are important for capture and clearance of modified LDL particles by monocytes/macrophages. Uptake via scavenger receptors is not regulated by intracellular levels of cholesterol and in consequence, macrophages develop into foam cells in the arterial intima. The levels of scavenger receptor CD36 are increased in atherosclerotic lesions and there is evidence that some components of oxLDL auto-regulate the expression of this receptor. Fcgamma receptor expression is increased in cardiovascular diseases but it is not known weather their expression is regulated by oxLDL. The biological properties of oxLDLs vary depending on the degree of oxidation. In the present study we investigated the effect of LDL particles showing extensive or low oxidation (HoxLDL and LoxLDL) on the expression of CD36 and FcgammaRII in a human monocytic cell line (THP-1), differentiated or not to macrophage, and the involvement of PPARgamma. It was found that both forms of oxLDL are able to increase the expression of CD36 and FcgammaRII and that this effect is dependent on the degree of oxidation and of the stage of cell differentiation (monocyte or macrophage). We also showed that the increased expression of FcgammaRII is dependent on PPARgamma whereas that of the CD36 is independent of PPARgamma.",
keywords = "Anilides/pharmacology, CD11b Antigen/metabolism, CD18 Antigens/metabolism, CD36 Antigens/metabolism, Cell Differentiation/drug effects, Cell Line, Chromatography, Ion Exchange, Fatty Acids, Unsaturated/metabolism, Humans, Lipoproteins, LDL/pharmacology, Monocytes/cytology, Oxidation-Reduction/drug effects, PPAR gamma/antagonists & inhibitors, Receptors, IgG/metabolism, Rosiglitazone, Tetradecanoylphorbol Acetate/pharmacology, Thiazolidinediones/pharmacology, Thiobarbituric Acid Reactive Substances/metabolism",
author = "Rios, {Francisco J O} and Sonia Jancar and Melo, {Ivo B} and Ketelhuth, {Daniel F J} and Magnus Gidlund",
note = "Copyright 2008 S. Karger AG, Basel.",
year = "2008",
doi = "10.1159/000185539",
language = "English",
volume = "22",
pages = "549--56",
journal = "Cellular Physiology and Biochemistry",
issn = "1015-8987",
publisher = "S. Karger AG",
number = "5-6",

}

Role of PPAR-gamma in the modulation of CD36 and FcgammaRII induced by LDL with low and high degrees of oxidation during the differentiation of the monocytic THP-1 cell line. / Rios, Francisco J O; Jancar, Sonia; Melo, Ivo B; Ketelhuth, Daniel F J; Gidlund, Magnus.

I: Cellular Physiology and Biochemistry, Bind 22, Nr. 5-6, 2008, s. 549-56.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Role of PPAR-gamma in the modulation of CD36 and FcgammaRII induced by LDL with low and high degrees of oxidation during the differentiation of the monocytic THP-1 cell line

AU - Rios, Francisco J O

AU - Jancar, Sonia

AU - Melo, Ivo B

AU - Ketelhuth, Daniel F J

AU - Gidlund, Magnus

N1 - Copyright 2008 S. Karger AG, Basel.

PY - 2008

Y1 - 2008

N2 - Scavenger or Fcgamma receptors are important for capture and clearance of modified LDL particles by monocytes/macrophages. Uptake via scavenger receptors is not regulated by intracellular levels of cholesterol and in consequence, macrophages develop into foam cells in the arterial intima. The levels of scavenger receptor CD36 are increased in atherosclerotic lesions and there is evidence that some components of oxLDL auto-regulate the expression of this receptor. Fcgamma receptor expression is increased in cardiovascular diseases but it is not known weather their expression is regulated by oxLDL. The biological properties of oxLDLs vary depending on the degree of oxidation. In the present study we investigated the effect of LDL particles showing extensive or low oxidation (HoxLDL and LoxLDL) on the expression of CD36 and FcgammaRII in a human monocytic cell line (THP-1), differentiated or not to macrophage, and the involvement of PPARgamma. It was found that both forms of oxLDL are able to increase the expression of CD36 and FcgammaRII and that this effect is dependent on the degree of oxidation and of the stage of cell differentiation (monocyte or macrophage). We also showed that the increased expression of FcgammaRII is dependent on PPARgamma whereas that of the CD36 is independent of PPARgamma.

AB - Scavenger or Fcgamma receptors are important for capture and clearance of modified LDL particles by monocytes/macrophages. Uptake via scavenger receptors is not regulated by intracellular levels of cholesterol and in consequence, macrophages develop into foam cells in the arterial intima. The levels of scavenger receptor CD36 are increased in atherosclerotic lesions and there is evidence that some components of oxLDL auto-regulate the expression of this receptor. Fcgamma receptor expression is increased in cardiovascular diseases but it is not known weather their expression is regulated by oxLDL. The biological properties of oxLDLs vary depending on the degree of oxidation. In the present study we investigated the effect of LDL particles showing extensive or low oxidation (HoxLDL and LoxLDL) on the expression of CD36 and FcgammaRII in a human monocytic cell line (THP-1), differentiated or not to macrophage, and the involvement of PPARgamma. It was found that both forms of oxLDL are able to increase the expression of CD36 and FcgammaRII and that this effect is dependent on the degree of oxidation and of the stage of cell differentiation (monocyte or macrophage). We also showed that the increased expression of FcgammaRII is dependent on PPARgamma whereas that of the CD36 is independent of PPARgamma.

KW - Anilides/pharmacology

KW - CD11b Antigen/metabolism

KW - CD18 Antigens/metabolism

KW - CD36 Antigens/metabolism

KW - Cell Differentiation/drug effects

KW - Cell Line

KW - Chromatography, Ion Exchange

KW - Fatty Acids, Unsaturated/metabolism

KW - Humans

KW - Lipoproteins, LDL/pharmacology

KW - Monocytes/cytology

KW - Oxidation-Reduction/drug effects

KW - PPAR gamma/antagonists & inhibitors

KW - Receptors, IgG/metabolism

KW - Rosiglitazone

KW - Tetradecanoylphorbol Acetate/pharmacology

KW - Thiazolidinediones/pharmacology

KW - Thiobarbituric Acid Reactive Substances/metabolism

U2 - 10.1159/000185539

DO - 10.1159/000185539

M3 - Journal article

C2 - 19088437

VL - 22

SP - 549

EP - 556

JO - Cellular Physiology and Biochemistry

JF - Cellular Physiology and Biochemistry

SN - 1015-8987

IS - 5-6

ER -