Role of B-cells in mycosis fungoides

Pia Rude Nielsen; Jens Ole Eriksen; Mia Dahl Sørensen; Ulrike Wehkamp; Lise Maria Lindahl; Michael Bzorek; Lars Iversen; Anders Woetman; Niels Ødum; Thomas Litman; Lise Mette Rahbek Gjerdrum

doi:10.2340/00015555-3775

Role of B-cells in mycosis fungoides

Pia Rude Nielsen^*
, Jens Ole Eriksen
, Mia Dahl Sørensen
, Ulrike Wehkamp
, Lise Maria Lindahl
, Michael Bzorek
, Lars Iversen
, Anders Woetman
, Niels Ødum
, Thomas Litman
, Lise Mette Rahbek Gjerdrum

^*Kontaktforfatter

KI, OUH, Forskningsenhed for Patologi (Odense)

Sjællands Universitetshospital, Roskilde
Aarhus Universitetshospital
Københavns Universitet
University Medical Center Schleswig-Holstein

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

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Abstract

Mycosis fungoides is the most common type of cutaneous T-cell lymphoma. The inflammatory microenvironment in mycosis fungoides is complex. There is accumulating evidence that the neoplastic T-cells take control of the microenvironment and thereby promote their own expansion by suppressing cellular immunity. B-cells have proved to be upregulated in large-cell transformed mycosis fungoides, and could potentially play a role in disease progression. To investigate the presence of B-cells in mycosis fungoides compared with controls, this study analysed 85 formalin-fixed and paraffin-embedded mycosis fungoides biopsies. MS4A1 gene expression was significantly upregulated in mycosis fungoides compared with controls (p < 0.0001) and further upregulated in disease progression, (p = 0.001). Digital quantification of PAX5⁺/ CD20⁺ cells confirmed the increased presence of Bcells in mycosis fungoides compared with controls. No co-labelling of CD3/CD20 was observed in the neoplastic T-cells. This study found a significantly increased presence of B-cells in the tumour-associated microenvironment in mycosis fungoides. These findings could potentially lead to new treatment strategies for mycosis fungoides.

Originalsprog	Engelsk
Artikelnummer	adv00413
Tidsskrift	Acta Dermato-Venereologica
Vol/bind	101
Udgave nummer	3
ISSN	0001-5555
DOI	https://doi.org/10.2340/00015555-3775
Status	Udgivet - 11. mar. 2021

Bibliografisk note

Funding Information:
LMRG has received funding from NanoString Technologies. T.L. is employed both by Copenhagen University and by LEO Pharma A/S. The remaining authors have no conflicts of interest to declare.

Funding Information:
This work was supported by the Danish Cancer Research Foundation (Dansk Kræftforskningsfond), Region Zealand Health and Research Foundation, the Region of Zealand and Southern Denmark Research Foundation, Aage Bang Foundation, LINAK A/S Nordborg, the Harboe Foundation, the carpenter Jørgen Holm and wife Elisa F. Hansen Memorial Foundation, the Medical Research Association Foundation and the A.P. Moeller Foundation.

Funding Information:
The technical assistance provided by the immunohistochemical and molecular team at the Department of Pathology, N?stved, Denmark is greatly appreciated. This work was supported by the Danish Cancer Research Foundation (Dansk Kr?ftforskningsfond), Region Zealand Health and Research Foundation, the Region of Zealand and Southern Denmark Research Foundation, Aage Bang Foundation, LINAK A/S Nordborg, the Harboe Foundation, the carpenter J?rgen Holm and wife Elisa F. Hansen Memorial Foundation, the Medical Research Association Foundation and the A.P. Moeller Foundation. LMRG has received funding from NanoString Technologies. T.L. is employed both by Copenhagen University and by LEO Pharma A/S. The remaining authors have no conflicts of interest to declare.

Finansiering

LMRG has received funding from NanoString Technologies. T.L. is employed both by Copenhagen University and by LEO Pharma A/S. The remaining authors have no conflicts of interest to declare. This work was supported by the Danish Cancer Research Foundation (Dansk Kræftforskningsfond), Region Zealand Health and Research Foundation, the Region of Zealand and Southern Denmark Research Foundation, Aage Bang Foundation, LINAK A/S Nordborg, the Harboe Foundation, the carpenter Jørgen Holm and wife Elisa F. Hansen Memorial Foundation, the Medical Research Association Foundation and the A.P. Moeller Foundation. The technical assistance provided by the immunohistochemical and molecular team at the Department of Pathology, N?stved, Denmark is greatly appreciated. This work was supported by the Danish Cancer Research Foundation (Dansk Kr?ftforskningsfond), Region Zealand Health and Research Foundation, the Region of Zealand and Southern Denmark Research Foundation, Aage Bang Foundation, LINAK A/S Nordborg, the Harboe Foundation, the carpenter J?rgen Holm and wife Elisa F. Hansen Memorial Foundation, the Medical Research Association Foundation and the A.P. Moeller Foundation. LMRG has received funding from NanoString Technologies. T.L. is employed both by Copenhagen University and by LEO Pharma A/S. The remaining authors have no conflicts of interest to declare.

Dokumenter og links

10.2340/00015555-3775Licens: CC BY-NC

Open Access VersionForlagets udgivne version, 3,25 MBLicens: CC BY-NC

SDU link

Tjek adgangen til materialet i Syddansk Universitetsbiblioteks søgemaskine

Citationsformater

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abstract = "Mycosis fungoides is the most common type of cutaneous T-cell lymphoma. The inflammatory microenvironment in mycosis fungoides is complex. There is accumulating evidence that the neoplastic T-cells take control of the microenvironment and thereby promote their own expansion by suppressing cellular immunity. B-cells have proved to be upregulated in large-cell transformed mycosis fungoides, and could potentially play a role in disease progression. To investigate the presence of B-cells in mycosis fungoides compared with controls, this study analysed 85 formalin-fixed and paraffin-embedded mycosis fungoides biopsies. MS4A1 gene expression was significantly upregulated in mycosis fungoides compared with controls (p < 0.0001) and further upregulated in disease progression, (p = 0.001). Digital quantification of PAX5+/ CD20+ cells confirmed the increased presence of Bcells in mycosis fungoides compared with controls. No co-labelling of CD3/CD20 was observed in the neoplastic T-cells. This study found a significantly increased presence of B-cells in the tumour-associated microenvironment in mycosis fungoides. These findings could potentially lead to new treatment strategies for mycosis fungoides.",

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AU - Nielsen, Pia Rude

AU - Eriksen, Jens Ole

AU - Sørensen, Mia Dahl

AU - Wehkamp, Ulrike

AU - Lindahl, Lise Maria

AU - Bzorek, Michael

AU - Iversen, Lars

AU - Woetman, Anders

AU - Ødum, Niels

AU - Litman, Thomas

AU - Gjerdrum, Lise Mette Rahbek

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N2 - Mycosis fungoides is the most common type of cutaneous T-cell lymphoma. The inflammatory microenvironment in mycosis fungoides is complex. There is accumulating evidence that the neoplastic T-cells take control of the microenvironment and thereby promote their own expansion by suppressing cellular immunity. B-cells have proved to be upregulated in large-cell transformed mycosis fungoides, and could potentially play a role in disease progression. To investigate the presence of B-cells in mycosis fungoides compared with controls, this study analysed 85 formalin-fixed and paraffin-embedded mycosis fungoides biopsies. MS4A1 gene expression was significantly upregulated in mycosis fungoides compared with controls (p < 0.0001) and further upregulated in disease progression, (p = 0.001). Digital quantification of PAX5+/ CD20+ cells confirmed the increased presence of Bcells in mycosis fungoides compared with controls. No co-labelling of CD3/CD20 was observed in the neoplastic T-cells. This study found a significantly increased presence of B-cells in the tumour-associated microenvironment in mycosis fungoides. These findings could potentially lead to new treatment strategies for mycosis fungoides.

AB - Mycosis fungoides is the most common type of cutaneous T-cell lymphoma. The inflammatory microenvironment in mycosis fungoides is complex. There is accumulating evidence that the neoplastic T-cells take control of the microenvironment and thereby promote their own expansion by suppressing cellular immunity. B-cells have proved to be upregulated in large-cell transformed mycosis fungoides, and could potentially play a role in disease progression. To investigate the presence of B-cells in mycosis fungoides compared with controls, this study analysed 85 formalin-fixed and paraffin-embedded mycosis fungoides biopsies. MS4A1 gene expression was significantly upregulated in mycosis fungoides compared with controls (p < 0.0001) and further upregulated in disease progression, (p = 0.001). Digital quantification of PAX5+/ CD20+ cells confirmed the increased presence of Bcells in mycosis fungoides compared with controls. No co-labelling of CD3/CD20 was observed in the neoplastic T-cells. This study found a significantly increased presence of B-cells in the tumour-associated microenvironment in mycosis fungoides. These findings could potentially lead to new treatment strategies for mycosis fungoides.

KW - B-cells

KW - Cutaneous t-cell lymphoma

KW - Mycosis fungoides

KW - Tumour microenvironment

U2 - 10.2340/00015555-3775

DO - 10.2340/00015555-3775

M3 - Journal article

C2 - 33686443

AN - SCOPUS:85102905080

SN - 0001-5555

VL - 101

JO - Acta Dermato-Venereologica

JF - Acta Dermato-Venereologica

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Role of B-cells in mycosis fungoides

Abstract

Bibliografisk note

Finansiering

Dokumenter og links

SDU link

Fingeraftryk

Citationsformater