RNA-Seq and Mass-Spectrometry-Based Lipidomics Reveal Extensive Changes of Glycerolipid Pathways in Brown Adipose Tissue in Response to Cold

Ann-Britt Marcher, Anne Loft, Ronni Nielsen, Terhi Vihervaara, Jesper Grud Skat Madsen, Marko Sysi-Aho, Kim Ekroos, Susanne Mandrup

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Resumé

Cold exposure greatly alters brown adipose tissue (BAT) gene expression and metabolism to increase thermogenic capacity. Here, we used RNA sequencing and mass-spectrometry-based lipidomics to provide acomprehensive resource describing the molecular signature of cold adaptation at the level of the transcriptome and lipidome. We show that short-term (3-day) cold exposure leads to a robust increase in expression of several brown adipocyte genes related to thermogenesis as well as the gene encoding the hormone irisin. However, pathway analysis shows that the most significantly induced genes are those involved in glycerophospholipid synthesis and fatty acid elongation. This is accompanied by
significant changes in the acyl chain composition of triacylglycerols (TAGs) as well as subspecies-selective changes of acyl chains in glycerophospholipids. These results indicate that cold adaptation of BAT is associated with significant and highly species-selective remodeling of both TAGs and glycerophospholipids.
OriginalsprogEngelsk
TidsskriftCell Reports
Vol/bind13
Udgave nummer9
Sider (fra-til)2000-2013
DOI
StatusUdgivet - 1. dec. 2015

Fingeraftryk

Glycerophospholipids
Mass spectrometry
RNA
Tissue
Triglycerides
Genes
Gene encoding
Brown Adipocytes
RNA Sequence Analysis
Metabolism
Gene expression
Elongation
Fatty Acids
Transcriptome
Hormones
Chemical analysis

Citer dette

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title = "RNA-Seq and Mass-Spectrometry-Based Lipidomics Reveal Extensive Changes of Glycerolipid Pathways in Brown Adipose Tissue in Response to Cold",
abstract = "Cold exposure greatly alters brown adipose tissue (BAT) gene expression and metabolism to increase thermogenic capacity. Here, we used RNA sequencing and mass-spectrometry-based lipidomics to provide a comprehensive resource describing the molecular signature of cold adaptation at the level of the transcriptome and lipidome. We show that short-term (3-day) cold exposure leads to a robust increase in expression of several brown adipocyte genes related to thermogenesis as well as the gene encoding the hormone irisin. However, pathway analysis shows that the most significantly induced genes are those involved in glycerophospholipid synthesis and fatty acid elongation. This is accompanied by significant changes in the acyl chain composition of triacylglycerols (TAGs) as well as subspecies-selective changes of acyl chains in glycerophospholipids. These results indicate that cold adaptation of BAT is associated with significant and highly species-selective remodeling of both TAGs and glycerophospholipids.",
author = "Ann-Britt Marcher and Anne Loft and Ronni Nielsen and Terhi Vihervaara and Madsen, {Jesper Grud Skat} and Marko Sysi-Aho and Kim Ekroos and Susanne Mandrup",
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year = "2015",
month = "12",
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doi = "10.1016/j.celrep.2015.10.069",
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RNA-Seq and Mass-Spectrometry-Based Lipidomics Reveal Extensive Changes of Glycerolipid Pathways in Brown Adipose Tissue in Response to Cold. / Marcher, Ann-Britt; Loft, Anne; Nielsen, Ronni; Vihervaara, Terhi; Madsen, Jesper Grud Skat; Sysi-Aho, Marko; Ekroos, Kim; Mandrup, Susanne.

I: Cell Reports, Bind 13, Nr. 9, 01.12.2015, s. 2000-2013.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - RNA-Seq and Mass-Spectrometry-Based Lipidomics Reveal Extensive Changes of Glycerolipid Pathways in Brown Adipose Tissue in Response to Cold

AU - Marcher, Ann-Britt

AU - Loft, Anne

AU - Nielsen, Ronni

AU - Vihervaara, Terhi

AU - Madsen, Jesper Grud Skat

AU - Sysi-Aho, Marko

AU - Ekroos, Kim

AU - Mandrup, Susanne

N1 - Copyright © 2015 The Authors. Published by Elsevier Inc. All rights reserved.

PY - 2015/12/1

Y1 - 2015/12/1

N2 - Cold exposure greatly alters brown adipose tissue (BAT) gene expression and metabolism to increase thermogenic capacity. Here, we used RNA sequencing and mass-spectrometry-based lipidomics to provide a comprehensive resource describing the molecular signature of cold adaptation at the level of the transcriptome and lipidome. We show that short-term (3-day) cold exposure leads to a robust increase in expression of several brown adipocyte genes related to thermogenesis as well as the gene encoding the hormone irisin. However, pathway analysis shows that the most significantly induced genes are those involved in glycerophospholipid synthesis and fatty acid elongation. This is accompanied by significant changes in the acyl chain composition of triacylglycerols (TAGs) as well as subspecies-selective changes of acyl chains in glycerophospholipids. These results indicate that cold adaptation of BAT is associated with significant and highly species-selective remodeling of both TAGs and glycerophospholipids.

AB - Cold exposure greatly alters brown adipose tissue (BAT) gene expression and metabolism to increase thermogenic capacity. Here, we used RNA sequencing and mass-spectrometry-based lipidomics to provide a comprehensive resource describing the molecular signature of cold adaptation at the level of the transcriptome and lipidome. We show that short-term (3-day) cold exposure leads to a robust increase in expression of several brown adipocyte genes related to thermogenesis as well as the gene encoding the hormone irisin. However, pathway analysis shows that the most significantly induced genes are those involved in glycerophospholipid synthesis and fatty acid elongation. This is accompanied by significant changes in the acyl chain composition of triacylglycerols (TAGs) as well as subspecies-selective changes of acyl chains in glycerophospholipids. These results indicate that cold adaptation of BAT is associated with significant and highly species-selective remodeling of both TAGs and glycerophospholipids.

U2 - 10.1016/j.celrep.2015.10.069

DO - 10.1016/j.celrep.2015.10.069

M3 - Journal article

C2 - 26628366

VL - 13

SP - 2000

EP - 2013

JO - Cell Reports

JF - Cell Reports

SN - 2211-1247

IS - 9

ER -