TY - JOUR
T1 - RNA decay by messenger RNA interferases
AU - Christensen-Dalsgaard, Mikkel
AU - Overgaard, Martin
AU - Winther, Kristoffer Skovbo
AU - Gerdes, Kenn
PY - 2008/1/1
Y1 - 2008/1/1
N2 - Two abundant toxin-antitoxin (TA) gene families, relBE and mazEF, encode mRNA cleaving enzymes whose ectopic overexpression abruptly inhibits translation and thereby induces a bacteriostatic condition. Here we describe and discuss protocols for the overproduction, purification, and analysis of mRNA cleaving enzymes such as RelE of Escherichia coli and the corresponding antitoxin RelB. In particular, we describe a set of plasmid vectors useful for the detailed analysis of cleavage sites in model mRNAs.
AB - Two abundant toxin-antitoxin (TA) gene families, relBE and mazEF, encode mRNA cleaving enzymes whose ectopic overexpression abruptly inhibits translation and thereby induces a bacteriostatic condition. Here we describe and discuss protocols for the overproduction, purification, and analysis of mRNA cleaving enzymes such as RelE of Escherichia coli and the corresponding antitoxin RelB. In particular, we describe a set of plasmid vectors useful for the detailed analysis of cleavage sites in model mRNAs.
U2 - 10.1016/S0076-6879(08)02225-8
DO - 10.1016/S0076-6879(08)02225-8
M3 - Journal article
C2 - 19161859
SN - 0076-6879
VL - 447
SP - 521
EP - 535
JO - Methods in Enzymology
JF - Methods in Enzymology
ER -