Ribosomal protein S6 phosphorylation and morphological changes in response to the tumour promoter 12-O-tetradecanoylphorbol 13-acetate in primary human tumour cells, established and transformed cell lines

A J Rance, M Thönnes, O G Issinger

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

Udgivelsesdato: 1985-Oct-30
OriginalsprogEngelsk
TidsskriftBBA General Subjects
Vol/bind847
Udgave nummer1
Sider (fra-til)128-131
Antal sider3
ISSN0304-4165
StatusUdgivet - 1985

Fingeraftryk

Ribosomal Protein S6
Transformed Cell Line
S 6
Fibroblasts
Neoplasms
Metabolic Networks and Pathways
Observation

Citer dette

@article{12c9b0505a4311de839d000ea68e967b,
title = "Ribosomal protein S6 phosphorylation and morphological changes in response to the tumour promoter 12-O-tetradecanoylphorbol 13-acetate in primary human tumour cells, established and transformed cell lines",
abstract = "The phosphorylation of ribosomal protein S6 in fibroblasts, primary human tumour cells, established and SV40-transformed human cell lines was compared after the addition of 12-O-tetradecanoylphorbol 13-acetate (TPA). In fibroblasts and primary tumour cell cultures, stimulation of S6 phosphorylation was about 4-6-fold. Established and transformed cell lines showed enhanced S6 phosphorylation which was not further stimulated by the addition of TPA. These findings indicated that the influence of TPA on the metabolic pathway, that finally leads to the phosphorylation of protein S6 in cells with a limited lifespan (fibroblasts, primary human tumour cells) can be mimicked by unknown steps also associated with immortalization (establishment function) and the transformed state of the tumour cells. Another interesting observation were morphological changes of the established and SV40-transformed cells which were visible as early as 20 min after the addition of TPA. In fibroblasts and primary tumour cells no changes in morphology were observed, even after 8h incubation.",
keywords = "Cell Line, Cell Transformation, Neoplastic, Cell Transformation, Viral, Fibroblasts, Glioma, Humans, Meningeal Neoplasms, Meningioma, Neoplasms, Phosphorylation, Ribosomal Proteins, Simian virus 40, Tetradecanoylphorbol Acetate",
author = "Rance, {A J} and M Th{\"o}nnes and Issinger, {O G}",
year = "1985",
language = "English",
volume = "847",
pages = "128--131",
journal = "B B A - General Subjects",
issn = "0304-4165",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - Ribosomal protein S6 phosphorylation and morphological changes in response to the tumour promoter 12-O-tetradecanoylphorbol 13-acetate in primary human tumour cells, established and transformed cell lines

AU - Rance, A J

AU - Thönnes, M

AU - Issinger, O G

PY - 1985

Y1 - 1985

N2 - The phosphorylation of ribosomal protein S6 in fibroblasts, primary human tumour cells, established and SV40-transformed human cell lines was compared after the addition of 12-O-tetradecanoylphorbol 13-acetate (TPA). In fibroblasts and primary tumour cell cultures, stimulation of S6 phosphorylation was about 4-6-fold. Established and transformed cell lines showed enhanced S6 phosphorylation which was not further stimulated by the addition of TPA. These findings indicated that the influence of TPA on the metabolic pathway, that finally leads to the phosphorylation of protein S6 in cells with a limited lifespan (fibroblasts, primary human tumour cells) can be mimicked by unknown steps also associated with immortalization (establishment function) and the transformed state of the tumour cells. Another interesting observation were morphological changes of the established and SV40-transformed cells which were visible as early as 20 min after the addition of TPA. In fibroblasts and primary tumour cells no changes in morphology were observed, even after 8h incubation.

AB - The phosphorylation of ribosomal protein S6 in fibroblasts, primary human tumour cells, established and SV40-transformed human cell lines was compared after the addition of 12-O-tetradecanoylphorbol 13-acetate (TPA). In fibroblasts and primary tumour cell cultures, stimulation of S6 phosphorylation was about 4-6-fold. Established and transformed cell lines showed enhanced S6 phosphorylation which was not further stimulated by the addition of TPA. These findings indicated that the influence of TPA on the metabolic pathway, that finally leads to the phosphorylation of protein S6 in cells with a limited lifespan (fibroblasts, primary human tumour cells) can be mimicked by unknown steps also associated with immortalization (establishment function) and the transformed state of the tumour cells. Another interesting observation were morphological changes of the established and SV40-transformed cells which were visible as early as 20 min after the addition of TPA. In fibroblasts and primary tumour cells no changes in morphology were observed, even after 8h incubation.

KW - Cell Line

KW - Cell Transformation, Neoplastic

KW - Cell Transformation, Viral

KW - Fibroblasts

KW - Glioma

KW - Humans

KW - Meningeal Neoplasms

KW - Meningioma

KW - Neoplasms

KW - Phosphorylation

KW - Ribosomal Proteins

KW - Simian virus 40

KW - Tetradecanoylphorbol Acetate

M3 - Journal article

C2 - 2996613

VL - 847

SP - 128

EP - 131

JO - B B A - General Subjects

JF - B B A - General Subjects

SN - 0304-4165

IS - 1

ER -