Responsive Emulsions for Sequential Multienzyme Cascades

Zhiyong Sun, Qingcai Zhao, Rainer Haag*, Changzhu Wu*


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Multienzyme cascade biocatalysis is an efficient synthetic process, avoiding the isolation/purification of intermediates and shifting the reaction equilibrium to the product side. However, multienzyme systems are often limited by their incompatibility and cross-reactivity. Herein, we report a multi-responsive emulsion to proceed multienzyme reactions sequentially for high reactivity. The emulsion is achieved using a CO2, pH, and thermo-responsive block copolymer as a stabilizer, allowing the on-demand control of emulsion morphology and phase composition. Applying this system to a three-step cascade reaction enables the individual optimal condition for each enzyme, and a high overall conversion (ca. 97 % of the calculated limit) is thereby obtained. Moreover, the multi-responsiveness of the emulsion allows the facile and separate yielding/recycling of products, polymers and active enzymes. Besides, the system could be scaled up with a good yield.

TidsskriftAngewandte Chemie International Edition
Udgave nummer15
Sider (fra-til)8410-8414
StatusUdgivet - 6. apr. 2021

Bibliografisk note

Funding Information:
We acknowledge financial support from the German Research Foundation and the Independent Research Fund Denmark. We acknowledge the assistance of the Core Facility BioSupraMol supported by the DFG. We thank Prof. Marion B. Ansorge‐Schumacher for valuable discussions and support. We thank Prof. Wolfgang Kroutil for providing the plasmid of ADH‐ht. Z.S. thanks the support from China Scholarship Council (CSC). Q.Z. thanks Dahlem Research School for financial support. Open access funding enabled and organized by Projekt DEAL.

Publisher Copyright:
© 2021 The Authors. Angewandte Chemie International Edition published by Wiley-VCH GmbH

Copyright 2021 Elsevier B.V., All rights reserved.


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