Response of midpiece vesicles on human sperm to osmotic stress

Joanna V Abraham-Peskir, Eric Chantler, Erik Uggerhøj, Jens Fedder

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

BACKGROUND: We investigated the osmotic response of midpiece vesicles (MPV) on human sperm.

METHODS: Light microscopy, transmission X-ray microscopy and computer-aided semen analysis was used to investigate sperm in normozoospermic semen from healthy donors, separated from semen and suspended in hyper- or hypo-osmotic solutions.

RESULTS: The mean incidence of MPV-bearing sperm in semen was 31% (range 8-71; n = 47). MPV were morphologically different from cytoplasmic droplets. The incidence of MPV-bearing sperm in separated populations increased reversibly after washing in Sperm Preparation Medium but not after washing in seminal plasma. There was an inverse relationship between medium osmolality and both MPV-bearing sperm incidence and MPV diameter. However, initial osmolality in semen from different donors did not correlate with incidence of MPV-bearing sperm. Furthermore, a direct relationship was observed in semen as osmolality increased with time. No correlation existed between progressive motility and semen osmolality. Progressive motility and the amplitude of lateral head displacement were significantly reduced in sperm with an MPV (three out of four semen samples, 26-32 sperm). The incidence of MPV-bearing sperm in those that had penetrated cervical mucus (75, 46, and 40%) was increased compared with the adjacent semen (24, 35, and 24%).

CONCLUSIONS: MPV are ubiquitous and distinct from cytoplasmic droplets. They respond to osmolality changes of the surrounding medium. The presence of an MPV can reduce motility but not survival in cervical mucus. Therefore, they should not be considered detrimental to sperm function.

OriginalsprogEngelsk
TidsskriftHuman Reproduction
Vol/bind17
Udgave nummer2
Sider (fra-til)375-82
Antal sider8
ISSN0268-1161
StatusUdgivet - feb. 2002

Fingeraftryk

Osmotic Pressure
Semen
Incidence
Cervix Mucus
Microscopy
X-Rays

Citer dette

Abraham-Peskir, J. V., Chantler, E., Uggerhøj, E., & Fedder, J. (2002). Response of midpiece vesicles on human sperm to osmotic stress. Human Reproduction, 17(2), 375-82.
Abraham-Peskir, Joanna V ; Chantler, Eric ; Uggerhøj, Erik ; Fedder, Jens. / Response of midpiece vesicles on human sperm to osmotic stress. I: Human Reproduction. 2002 ; Bind 17, Nr. 2. s. 375-82.
@article{859fd9c2ca194a2ba86d6f91eda75a33,
title = "Response of midpiece vesicles on human sperm to osmotic stress",
abstract = "BACKGROUND: We investigated the osmotic response of midpiece vesicles (MPV) on human sperm.METHODS: Light microscopy, transmission X-ray microscopy and computer-aided semen analysis was used to investigate sperm in normozoospermic semen from healthy donors, separated from semen and suspended in hyper- or hypo-osmotic solutions.RESULTS: The mean incidence of MPV-bearing sperm in semen was 31{\%} (range 8-71; n = 47). MPV were morphologically different from cytoplasmic droplets. The incidence of MPV-bearing sperm in separated populations increased reversibly after washing in Sperm Preparation Medium but not after washing in seminal plasma. There was an inverse relationship between medium osmolality and both MPV-bearing sperm incidence and MPV diameter. However, initial osmolality in semen from different donors did not correlate with incidence of MPV-bearing sperm. Furthermore, a direct relationship was observed in semen as osmolality increased with time. No correlation existed between progressive motility and semen osmolality. Progressive motility and the amplitude of lateral head displacement were significantly reduced in sperm with an MPV (three out of four semen samples, 26-32 sperm). The incidence of MPV-bearing sperm in those that had penetrated cervical mucus (75, 46, and 40{\%}) was increased compared with the adjacent semen (24, 35, and 24{\%}).CONCLUSIONS: MPV are ubiquitous and distinct from cytoplasmic droplets. They respond to osmolality changes of the surrounding medium. The presence of an MPV can reduce motility but not survival in cervical mucus. Therefore, they should not be considered detrimental to sperm function.",
keywords = "Cervix Mucus, Humans, Male, Osmolar Concentration, Osmotic Pressure, Radiography, Reference Values, Semen, Sperm Motility, Spermatozoa, Stress, Physiological, Time Factors, Journal Article, Research Support, Non-U.S. Gov't",
author = "Abraham-Peskir, {Joanna V} and Eric Chantler and Erik Uggerh{\o}j and Jens Fedder",
year = "2002",
month = "2",
language = "English",
volume = "17",
pages = "375--82",
journal = "Human Reproduction",
issn = "0268-1161",
publisher = "Heinemann",
number = "2",

}

Abraham-Peskir, JV, Chantler, E, Uggerhøj, E & Fedder, J 2002, 'Response of midpiece vesicles on human sperm to osmotic stress', Human Reproduction, bind 17, nr. 2, s. 375-82.

Response of midpiece vesicles on human sperm to osmotic stress. / Abraham-Peskir, Joanna V; Chantler, Eric; Uggerhøj, Erik; Fedder, Jens.

I: Human Reproduction, Bind 17, Nr. 2, 02.2002, s. 375-82.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Response of midpiece vesicles on human sperm to osmotic stress

AU - Abraham-Peskir, Joanna V

AU - Chantler, Eric

AU - Uggerhøj, Erik

AU - Fedder, Jens

PY - 2002/2

Y1 - 2002/2

N2 - BACKGROUND: We investigated the osmotic response of midpiece vesicles (MPV) on human sperm.METHODS: Light microscopy, transmission X-ray microscopy and computer-aided semen analysis was used to investigate sperm in normozoospermic semen from healthy donors, separated from semen and suspended in hyper- or hypo-osmotic solutions.RESULTS: The mean incidence of MPV-bearing sperm in semen was 31% (range 8-71; n = 47). MPV were morphologically different from cytoplasmic droplets. The incidence of MPV-bearing sperm in separated populations increased reversibly after washing in Sperm Preparation Medium but not after washing in seminal plasma. There was an inverse relationship between medium osmolality and both MPV-bearing sperm incidence and MPV diameter. However, initial osmolality in semen from different donors did not correlate with incidence of MPV-bearing sperm. Furthermore, a direct relationship was observed in semen as osmolality increased with time. No correlation existed between progressive motility and semen osmolality. Progressive motility and the amplitude of lateral head displacement were significantly reduced in sperm with an MPV (three out of four semen samples, 26-32 sperm). The incidence of MPV-bearing sperm in those that had penetrated cervical mucus (75, 46, and 40%) was increased compared with the adjacent semen (24, 35, and 24%).CONCLUSIONS: MPV are ubiquitous and distinct from cytoplasmic droplets. They respond to osmolality changes of the surrounding medium. The presence of an MPV can reduce motility but not survival in cervical mucus. Therefore, they should not be considered detrimental to sperm function.

AB - BACKGROUND: We investigated the osmotic response of midpiece vesicles (MPV) on human sperm.METHODS: Light microscopy, transmission X-ray microscopy and computer-aided semen analysis was used to investigate sperm in normozoospermic semen from healthy donors, separated from semen and suspended in hyper- or hypo-osmotic solutions.RESULTS: The mean incidence of MPV-bearing sperm in semen was 31% (range 8-71; n = 47). MPV were morphologically different from cytoplasmic droplets. The incidence of MPV-bearing sperm in separated populations increased reversibly after washing in Sperm Preparation Medium but not after washing in seminal plasma. There was an inverse relationship between medium osmolality and both MPV-bearing sperm incidence and MPV diameter. However, initial osmolality in semen from different donors did not correlate with incidence of MPV-bearing sperm. Furthermore, a direct relationship was observed in semen as osmolality increased with time. No correlation existed between progressive motility and semen osmolality. Progressive motility and the amplitude of lateral head displacement were significantly reduced in sperm with an MPV (three out of four semen samples, 26-32 sperm). The incidence of MPV-bearing sperm in those that had penetrated cervical mucus (75, 46, and 40%) was increased compared with the adjacent semen (24, 35, and 24%).CONCLUSIONS: MPV are ubiquitous and distinct from cytoplasmic droplets. They respond to osmolality changes of the surrounding medium. The presence of an MPV can reduce motility but not survival in cervical mucus. Therefore, they should not be considered detrimental to sperm function.

KW - Cervix Mucus

KW - Humans

KW - Male

KW - Osmolar Concentration

KW - Osmotic Pressure

KW - Radiography

KW - Reference Values

KW - Semen

KW - Sperm Motility

KW - Spermatozoa

KW - Stress, Physiological

KW - Time Factors

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

M3 - Journal article

C2 - 11821282

VL - 17

SP - 375

EP - 382

JO - Human Reproduction

JF - Human Reproduction

SN - 0268-1161

IS - 2

ER -

Abraham-Peskir JV, Chantler E, Uggerhøj E, Fedder J. Response of midpiece vesicles on human sperm to osmotic stress. Human Reproduction. 2002 feb;17(2):375-82.