Renal nerves and nNOS: roles in natriuresis of acute isovolumetric sodium loading in conscious rats

Elzbieta Kompanowska-Jezierska, Helle Wolff, Marta Kuczeriszka, Jan B Gramsbergen, Agnieszka Walkowska, Edward J Johns, Peter Bie

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

Udgivelsesdato: Apr
OriginalsprogEngelsk
TidsskriftAmerican Journal of Physiology: Regulatory, Integrative and Comparative Physiology
Vol/bind294
Udgave nummer4
Sider (fra-til)R1130-9
ISSN0363-6119
DOI
StatusUdgivet - 1. apr. 2008

Fingeraftryk

Natriuresis
Kidney
Renin
Hypertonic Solutions
Intravenous Infusions
Arterial Pressure
Down-Regulation

Citer dette

Kompanowska-Jezierska, Elzbieta ; Wolff, Helle ; Kuczeriszka, Marta ; Gramsbergen, Jan B ; Walkowska, Agnieszka ; Johns, Edward J ; Bie, Peter. / Renal nerves and nNOS : roles in natriuresis of acute isovolumetric sodium loading in conscious rats. I: American Journal of Physiology: Regulatory, Integrative and Comparative Physiology. 2008 ; Bind 294, Nr. 4. s. R1130-9.
@article{8b7d7570622511ddb1a1000ea68e967b,
title = "Renal nerves and nNOS: roles in natriuresis of acute isovolumetric sodium loading in conscious rats",
abstract = "It was hypothesized that renal sympathetic nerve activity (RSNA) and neuronal nitric oxide synthase (nNOS) are involved in the acute inhibition of renin secretion and the natriuresis following slow NaCl loading (NaLoad) and that RSNA participates in the regulation of arterial blood pressure (MABP). This was tested by NaLoad after chronic renal denervation with and without inhibition of nNOS by S-methyl-thiocitrulline (SMTC). In addition, the acute effects of renal denervation on MABP and sodium balance were assessed. Rats were investigated in the conscious, catheterized state, in metabolic cages, and acutely during anesthesia. NaLoad was performed over 2 h by intravenous infusion of hypertonic solution (50 micromol.min(-1).kg body mass(-1)) at constant body volume conditions. SMTC was coinfused in amounts (20 microg.min(-1).kg(-1)) reported to selectively inhibit nNOS. Directly measured MABPs of acutely and chronically denervated rats were less than control (15{\%} and 9{\%}, respectively, P < 0.005). Plasma renin concentration (PRC) was reduced by renal denervation (14.5 +/- 0.2 vs. 19.3 +/- 1.3 mIU/l, P < 0.005) and by nNOS inhibition (12.4 +/- 2.3 vs. 19.6 +/- 1.6 mlU/l, P < 0.005). NaLoad reduced PRC (P < 0.05) and elevated MABP modestly (P < 0.05) and increased sodium excretion six-fold, irrespective of renal denervation and SMTC. The metabolic data demonstrated that renal denervation lowered sodium balance during the first days after denervation (P < 0.001). These data show that renal denervation decreases MABP and renin secretion. However, neither renal denervation nor nNOS inhibition affects either the renin down-regulation or the natriuretic response to acute sodium loading. Acute sodium-driven renin regulation seems independent of RSNA and nNOS under the present conditions.",
keywords = "Animals, Blood Pressure, Citrulline, Consciousness, Enzyme Inhibitors, Female, Glomerular Filtration Rate, Infusions, Intravenous, Kidney, Natriuresis, Nitric Oxide Synthase, Norepinephrine, Rats, Rats, Sprague-Dawley, Renin, Saline Solution, Hypertonic, Sympathectomy, Sympathetic Nervous System, Thiourea, Time Factors, Water-Electrolyte Balance",
author = "Elzbieta Kompanowska-Jezierska and Helle Wolff and Marta Kuczeriszka and Gramsbergen, {Jan B} and Agnieszka Walkowska and Johns, {Edward J} and Peter Bie",
year = "2008",
month = "4",
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doi = "10.1152/ajpregu.00908.2007",
language = "English",
volume = "294",
pages = "R1130--9",
journal = "American Journal of Physiology: Regulatory, Integrative and Comparative Physiology",
issn = "0363-6119",
publisher = "American Physiological Society",
number = "4",

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Renal nerves and nNOS : roles in natriuresis of acute isovolumetric sodium loading in conscious rats. / Kompanowska-Jezierska, Elzbieta; Wolff, Helle; Kuczeriszka, Marta; Gramsbergen, Jan B; Walkowska, Agnieszka; Johns, Edward J; Bie, Peter.

I: American Journal of Physiology: Regulatory, Integrative and Comparative Physiology, Bind 294, Nr. 4, 01.04.2008, s. R1130-9.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Renal nerves and nNOS

T2 - roles in natriuresis of acute isovolumetric sodium loading in conscious rats

AU - Kompanowska-Jezierska, Elzbieta

AU - Wolff, Helle

AU - Kuczeriszka, Marta

AU - Gramsbergen, Jan B

AU - Walkowska, Agnieszka

AU - Johns, Edward J

AU - Bie, Peter

PY - 2008/4/1

Y1 - 2008/4/1

N2 - It was hypothesized that renal sympathetic nerve activity (RSNA) and neuronal nitric oxide synthase (nNOS) are involved in the acute inhibition of renin secretion and the natriuresis following slow NaCl loading (NaLoad) and that RSNA participates in the regulation of arterial blood pressure (MABP). This was tested by NaLoad after chronic renal denervation with and without inhibition of nNOS by S-methyl-thiocitrulline (SMTC). In addition, the acute effects of renal denervation on MABP and sodium balance were assessed. Rats were investigated in the conscious, catheterized state, in metabolic cages, and acutely during anesthesia. NaLoad was performed over 2 h by intravenous infusion of hypertonic solution (50 micromol.min(-1).kg body mass(-1)) at constant body volume conditions. SMTC was coinfused in amounts (20 microg.min(-1).kg(-1)) reported to selectively inhibit nNOS. Directly measured MABPs of acutely and chronically denervated rats were less than control (15% and 9%, respectively, P < 0.005). Plasma renin concentration (PRC) was reduced by renal denervation (14.5 +/- 0.2 vs. 19.3 +/- 1.3 mIU/l, P < 0.005) and by nNOS inhibition (12.4 +/- 2.3 vs. 19.6 +/- 1.6 mlU/l, P < 0.005). NaLoad reduced PRC (P < 0.05) and elevated MABP modestly (P < 0.05) and increased sodium excretion six-fold, irrespective of renal denervation and SMTC. The metabolic data demonstrated that renal denervation lowered sodium balance during the first days after denervation (P < 0.001). These data show that renal denervation decreases MABP and renin secretion. However, neither renal denervation nor nNOS inhibition affects either the renin down-regulation or the natriuretic response to acute sodium loading. Acute sodium-driven renin regulation seems independent of RSNA and nNOS under the present conditions.

AB - It was hypothesized that renal sympathetic nerve activity (RSNA) and neuronal nitric oxide synthase (nNOS) are involved in the acute inhibition of renin secretion and the natriuresis following slow NaCl loading (NaLoad) and that RSNA participates in the regulation of arterial blood pressure (MABP). This was tested by NaLoad after chronic renal denervation with and without inhibition of nNOS by S-methyl-thiocitrulline (SMTC). In addition, the acute effects of renal denervation on MABP and sodium balance were assessed. Rats were investigated in the conscious, catheterized state, in metabolic cages, and acutely during anesthesia. NaLoad was performed over 2 h by intravenous infusion of hypertonic solution (50 micromol.min(-1).kg body mass(-1)) at constant body volume conditions. SMTC was coinfused in amounts (20 microg.min(-1).kg(-1)) reported to selectively inhibit nNOS. Directly measured MABPs of acutely and chronically denervated rats were less than control (15% and 9%, respectively, P < 0.005). Plasma renin concentration (PRC) was reduced by renal denervation (14.5 +/- 0.2 vs. 19.3 +/- 1.3 mIU/l, P < 0.005) and by nNOS inhibition (12.4 +/- 2.3 vs. 19.6 +/- 1.6 mlU/l, P < 0.005). NaLoad reduced PRC (P < 0.05) and elevated MABP modestly (P < 0.05) and increased sodium excretion six-fold, irrespective of renal denervation and SMTC. The metabolic data demonstrated that renal denervation lowered sodium balance during the first days after denervation (P < 0.001). These data show that renal denervation decreases MABP and renin secretion. However, neither renal denervation nor nNOS inhibition affects either the renin down-regulation or the natriuretic response to acute sodium loading. Acute sodium-driven renin regulation seems independent of RSNA and nNOS under the present conditions.

KW - Animals

KW - Blood Pressure

KW - Citrulline

KW - Consciousness

KW - Enzyme Inhibitors

KW - Female

KW - Glomerular Filtration Rate

KW - Infusions, Intravenous

KW - Kidney

KW - Natriuresis

KW - Nitric Oxide Synthase

KW - Norepinephrine

KW - Rats

KW - Rats, Sprague-Dawley

KW - Renin

KW - Saline Solution, Hypertonic

KW - Sympathectomy

KW - Sympathetic Nervous System

KW - Thiourea

KW - Time Factors

KW - Water-Electrolyte Balance

U2 - 10.1152/ajpregu.00908.2007

DO - 10.1152/ajpregu.00908.2007

M3 - Journal article

C2 - 18234741

VL - 294

SP - R1130-9

JO - American Journal of Physiology: Regulatory, Integrative and Comparative Physiology

JF - American Journal of Physiology: Regulatory, Integrative and Comparative Physiology

SN - 0363-6119

IS - 4

ER -