Regulatory elements in the promoter region of the rat gene encoding the acyl-CoA-binding protein

M Elholm, G Bjerking, J Knudsen, K Kristiansen, S Mandrup

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

Udgivelsesdato: 1996-Sep-16
OriginalsprogEngelsk
TidsskriftGene
Vol/bind173
Udgave nummer2
Sider (fra-til)233-238
Antal sider5
ISSN0378-1119
DOI
StatusUdgivet - 16. sep. 1996

Fingeraftryk

Diazepam Binding Inhibitor
Retinoid X Receptor alpha
Peroxisome Proliferators
PPAR gamma
PPAR delta
PPAR alpha
Liver Extracts
Essential Genes
Transcription Factor AP-1
Electrophoretic Mobility Shift Assay
Hepatocellular Carcinoma
Messenger RNA

Citer dette

Elholm, M ; Bjerking, G ; Knudsen, J ; Kristiansen, K ; Mandrup, S. / Regulatory elements in the promoter region of the rat gene encoding the acyl-CoA-binding protein. I: Gene. 1996 ; Bind 173, Nr. 2. s. 233-238.
@article{18286b107d1911de9c46000ea68e967b,
title = "Regulatory elements in the promoter region of the rat gene encoding the acyl-CoA-binding protein",
abstract = "Acyl-CoA-binding protein (ACBP) is an ubiquitously expressed 10-kDa protein which is present in high amounts in cells involved in solute transport or secretion. Rat ACBP is encoded by a gene containing the typical hallmarks of a housekeeping gene. Analysis of the promoter region of the rat ACBP gene by electrophoretic mobility shift assay (EMSA) revealed specific binding of proteins from rat liver nuclear extracts to potential recognition sequences of NF-1/CTF, Sp1, AP-1, C/EBP and HNF-3. In addition, specific binding to a DR-1 type element was observed. By using in vitro translated peroxisome proliferator activated receptors (PPAR) and a retinoid X receptor alpha (RXRalpha), we demonstrated that this DR-1 element was capable of binding PPARalpha/RXRalpha, PPARdelta/RXRalpha and PPARgamma2/RXRalpha heterodimers. The PPARgamma2/RXRalpha heterodimer appeared to have the highest affinity for the ACBP DR-1 element. Addition of peroxisome proliferators (PP) to H4IIEC3 rat hepatoma cells led to an increase in the ACBP mRNA level, indicating that the DR-1 element could be a functional peroxisome proliferator responsive element (PPRE). Analysis of the ACBP promoter by transient transfection showed that deletion of the region containing the DR-1 element reduced transcriptional activity, and further indicated that three AP-2 sites and one NF-1/CTF site in the proximal promoter are of importance for basal promoter activity.",
keywords = "Animals, Binding Sites, Carrier Proteins, Diazepam Binding Inhibitor, Electrophoresis, Polyacrylamide Gel, Gene Expression, Phosphoproteins, Promoter Regions, Genetic, Pyrimidines, Rats, Rats, Sprague-Dawley, Transcription Factors, Transfection, Tumor Cells, Cultured",
author = "M Elholm and G Bjerking and J Knudsen and K Kristiansen and S Mandrup",
year = "1996",
month = "9",
day = "16",
doi = "10.1016/0378-1119(96)00213-2",
language = "English",
volume = "173",
pages = "233--238",
journal = "Gene",
issn = "0378-1119",
publisher = "Elsevier",
number = "2",

}

Regulatory elements in the promoter region of the rat gene encoding the acyl-CoA-binding protein. / Elholm, M; Bjerking, G; Knudsen, J; Kristiansen, K; Mandrup, S.

I: Gene, Bind 173, Nr. 2, 16.09.1996, s. 233-238.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Regulatory elements in the promoter region of the rat gene encoding the acyl-CoA-binding protein

AU - Elholm, M

AU - Bjerking, G

AU - Knudsen, J

AU - Kristiansen, K

AU - Mandrup, S

PY - 1996/9/16

Y1 - 1996/9/16

N2 - Acyl-CoA-binding protein (ACBP) is an ubiquitously expressed 10-kDa protein which is present in high amounts in cells involved in solute transport or secretion. Rat ACBP is encoded by a gene containing the typical hallmarks of a housekeeping gene. Analysis of the promoter region of the rat ACBP gene by electrophoretic mobility shift assay (EMSA) revealed specific binding of proteins from rat liver nuclear extracts to potential recognition sequences of NF-1/CTF, Sp1, AP-1, C/EBP and HNF-3. In addition, specific binding to a DR-1 type element was observed. By using in vitro translated peroxisome proliferator activated receptors (PPAR) and a retinoid X receptor alpha (RXRalpha), we demonstrated that this DR-1 element was capable of binding PPARalpha/RXRalpha, PPARdelta/RXRalpha and PPARgamma2/RXRalpha heterodimers. The PPARgamma2/RXRalpha heterodimer appeared to have the highest affinity for the ACBP DR-1 element. Addition of peroxisome proliferators (PP) to H4IIEC3 rat hepatoma cells led to an increase in the ACBP mRNA level, indicating that the DR-1 element could be a functional peroxisome proliferator responsive element (PPRE). Analysis of the ACBP promoter by transient transfection showed that deletion of the region containing the DR-1 element reduced transcriptional activity, and further indicated that three AP-2 sites and one NF-1/CTF site in the proximal promoter are of importance for basal promoter activity.

AB - Acyl-CoA-binding protein (ACBP) is an ubiquitously expressed 10-kDa protein which is present in high amounts in cells involved in solute transport or secretion. Rat ACBP is encoded by a gene containing the typical hallmarks of a housekeeping gene. Analysis of the promoter region of the rat ACBP gene by electrophoretic mobility shift assay (EMSA) revealed specific binding of proteins from rat liver nuclear extracts to potential recognition sequences of NF-1/CTF, Sp1, AP-1, C/EBP and HNF-3. In addition, specific binding to a DR-1 type element was observed. By using in vitro translated peroxisome proliferator activated receptors (PPAR) and a retinoid X receptor alpha (RXRalpha), we demonstrated that this DR-1 element was capable of binding PPARalpha/RXRalpha, PPARdelta/RXRalpha and PPARgamma2/RXRalpha heterodimers. The PPARgamma2/RXRalpha heterodimer appeared to have the highest affinity for the ACBP DR-1 element. Addition of peroxisome proliferators (PP) to H4IIEC3 rat hepatoma cells led to an increase in the ACBP mRNA level, indicating that the DR-1 element could be a functional peroxisome proliferator responsive element (PPRE). Analysis of the ACBP promoter by transient transfection showed that deletion of the region containing the DR-1 element reduced transcriptional activity, and further indicated that three AP-2 sites and one NF-1/CTF site in the proximal promoter are of importance for basal promoter activity.

KW - Animals

KW - Binding Sites

KW - Carrier Proteins

KW - Diazepam Binding Inhibitor

KW - Electrophoresis, Polyacrylamide Gel

KW - Gene Expression

KW - Phosphoproteins

KW - Promoter Regions, Genetic

KW - Pyrimidines

KW - Rats

KW - Rats, Sprague-Dawley

KW - Transcription Factors

KW - Transfection

KW - Tumor Cells, Cultured

U2 - 10.1016/0378-1119(96)00213-2

DO - 10.1016/0378-1119(96)00213-2

M3 - Journal article

VL - 173

SP - 233

EP - 238

JO - Gene

JF - Gene

SN - 0378-1119

IS - 2

ER -