Regulation of Na+/K+-ATPase activity by nitric oxide in the kidney and gill of the brown trout (Salmo trutta).

Christian K Tipsmark, Steffen S Madsen

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In teleost fish, successful osmoregulation involves controlled ion transport mechanisms in kidney and gill epithelia. In this study, the effect of nitric oxide (NO) on Na(+)/K(+)-ATPase was investigated in vitro in these two tissues in brown trout (Salmo trutta) acclimated to freshwater. The NO donor sodium nitroprusside (SNP) inhibited in situ Na(+)/K(+)-ATPase activity, measured as ouabain-sensitive Rb(+) uptake, in both samples of kidney and gill tissue and in isolated gill cells. The effect was dose-dependent in both tissues, with a maximal observed inhibition of approximately 40-50% (1 mmol l (-1) SNP). The time-course of inhibition revealed a maximum effect with 10 min pre-incubation. The effect of SNP was reproduced with another NO donor, papa-nonoate (NOC-15; 200 micro mol l(-1)), and was prevented by the NO scavenger 2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl 3-oxide (PTIO; 1 mmol l(-1)). To further investigate the mechanism of the NO effect, whole-tissue Na(+) and K(+) levels were analysed. In kidney, SNP (1 mmol l(-1)) led to an increase in tissue Na(+) levels and a decrease in K(+) levels in a 3:2 ratio. In gill tissue, no change in either ion was observed. These observations indicate that the effect on Na(+)/K(+)-ATPase is direct rather than due to a decrease in intracellular Na(+), its rate-limiting substrate. SNP elevated the level of cyclic GMP (cGMP) in both kidney and gill tissue. Dibutyryl cyclic GMP (db-cGMP; 1 mmol l(-1)) also inhibited Na(+)/K(+)-ATPase activity in both tissues. Hence, a possible mechanism may involve the cGMP-activated kinase, even though other mechanisms cannot be excluded.
Udgivelsesdato: 2003-May
TidsskriftJournal of Experimental Biology
Udgave nummerPt 9
Sider (fra-til)1503-1510
Antal sider7
StatusUdgivet - 1. maj 2003