Reduced expression of C5a receptors on neutrophils from cord blood

Mads Nybo, O Sørensen, R Leslie, P Wang

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

AIM: To describe further functional deficiencies of neonatal neutrophils by measuring the expression of C5a receptors.

METHODS: C5a uptake was measured using flow cytometry with fluorescein isothiocynate labelled recombinant C5a. The response of neutrophils to stimulation with C5a and fMLP was tested by measuring migration and exocytosis of myeloperoxidase and lactoferrin.

RESULTS: C5a mean fluorescence on neutrophils from neonates was significantly lower (22.4 (SD 3.5)) than in adult controls (31.5 (3.1)). Neutrophils from neonates migrated poorly towards both C5a and fMLP compared with those from adult controls. Exocytosis of myeloperoxidase, but not lactoferrin from neonatal neutrophils stimulated with C5a, was significantly lower than in adult controls. fMLP stimulation, on the other hand, resulted in significantly higher exocytosis in neonates.

CONCLUSION: The lower expression of C5a receptors on neutrophils from neonates could be related to reduced C5a mediated exocytosis of myeloperoxidase.

OriginalsprogEngelsk
TidsskriftArchives of Disease in Childhood. Fetal and Neonatal Edition
Vol/bind78
Udgave nummer2
Sider (fra-til)F129-32
ISSN1359-2998
StatusUdgivet - mar. 1998

Fingeraftryk

Anaphylatoxin C5a Receptor
Fetal Blood
Neutrophils
Exocytosis
Peroxidase
Lactoferrin
Fluorescein
Flow Cytometry
Fluorescence

Citer dette

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title = "Reduced expression of C5a receptors on neutrophils from cord blood",
abstract = "AIM: To describe further functional deficiencies of neonatal neutrophils by measuring the expression of C5a receptors.METHODS: C5a uptake was measured using flow cytometry with fluorescein isothiocynate labelled recombinant C5a. The response of neutrophils to stimulation with C5a and fMLP was tested by measuring migration and exocytosis of myeloperoxidase and lactoferrin.RESULTS: C5a mean fluorescence on neutrophils from neonates was significantly lower (22.4 (SD 3.5)) than in adult controls (31.5 (3.1)). Neutrophils from neonates migrated poorly towards both C5a and fMLP compared with those from adult controls. Exocytosis of myeloperoxidase, but not lactoferrin from neonatal neutrophils stimulated with C5a, was significantly lower than in adult controls. fMLP stimulation, on the other hand, resulted in significantly higher exocytosis in neonates.CONCLUSION: The lower expression of C5a receptors on neutrophils from neonates could be related to reduced C5a mediated exocytosis of myeloperoxidase.",
keywords = "Antigens, CD, Cells, Cultured, Complement C5a, Fetal Blood, Flow Cytometry, Humans, Infant, Newborn, N-Formylmethionine Leucyl-Phenylalanine, Neutrophils, Peroxidase, Receptor, Anaphylatoxin C5a, Receptors, Complement",
author = "Mads Nybo and O S{\o}rensen and R Leslie and P Wang",
year = "1998",
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journal = "Archives of Disease in Childhood. Fetal and Neonatal Edition",
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Reduced expression of C5a receptors on neutrophils from cord blood. / Nybo, Mads; Sørensen, O; Leslie, R; Wang, P.

I: Archives of Disease in Childhood. Fetal and Neonatal Edition, Bind 78, Nr. 2, 03.1998, s. F129-32.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Reduced expression of C5a receptors on neutrophils from cord blood

AU - Nybo, Mads

AU - Sørensen, O

AU - Leslie, R

AU - Wang, P

PY - 1998/3

Y1 - 1998/3

N2 - AIM: To describe further functional deficiencies of neonatal neutrophils by measuring the expression of C5a receptors.METHODS: C5a uptake was measured using flow cytometry with fluorescein isothiocynate labelled recombinant C5a. The response of neutrophils to stimulation with C5a and fMLP was tested by measuring migration and exocytosis of myeloperoxidase and lactoferrin.RESULTS: C5a mean fluorescence on neutrophils from neonates was significantly lower (22.4 (SD 3.5)) than in adult controls (31.5 (3.1)). Neutrophils from neonates migrated poorly towards both C5a and fMLP compared with those from adult controls. Exocytosis of myeloperoxidase, but not lactoferrin from neonatal neutrophils stimulated with C5a, was significantly lower than in adult controls. fMLP stimulation, on the other hand, resulted in significantly higher exocytosis in neonates.CONCLUSION: The lower expression of C5a receptors on neutrophils from neonates could be related to reduced C5a mediated exocytosis of myeloperoxidase.

AB - AIM: To describe further functional deficiencies of neonatal neutrophils by measuring the expression of C5a receptors.METHODS: C5a uptake was measured using flow cytometry with fluorescein isothiocynate labelled recombinant C5a. The response of neutrophils to stimulation with C5a and fMLP was tested by measuring migration and exocytosis of myeloperoxidase and lactoferrin.RESULTS: C5a mean fluorescence on neutrophils from neonates was significantly lower (22.4 (SD 3.5)) than in adult controls (31.5 (3.1)). Neutrophils from neonates migrated poorly towards both C5a and fMLP compared with those from adult controls. Exocytosis of myeloperoxidase, but not lactoferrin from neonatal neutrophils stimulated with C5a, was significantly lower than in adult controls. fMLP stimulation, on the other hand, resulted in significantly higher exocytosis in neonates.CONCLUSION: The lower expression of C5a receptors on neutrophils from neonates could be related to reduced C5a mediated exocytosis of myeloperoxidase.

KW - Antigens, CD

KW - Cells, Cultured

KW - Complement C5a

KW - Fetal Blood

KW - Flow Cytometry

KW - Humans

KW - Infant, Newborn

KW - N-Formylmethionine Leucyl-Phenylalanine

KW - Neutrophils

KW - Peroxidase

KW - Receptor, Anaphylatoxin C5a

KW - Receptors, Complement

M3 - Journal article

C2 - 9577284

VL - 78

SP - F129-32

JO - Archives of Disease in Childhood. Fetal and Neonatal Edition

JF - Archives of Disease in Childhood. Fetal and Neonatal Edition

SN - 1359-2998

IS - 2

ER -