Reduced complement-mediated immune complex solubilizing capacity and the presence of incompletely solubilized immune complexes in SLE sera

Gunnar Baatrup, I Petersen, J C Jensenius, S E Svehag

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

    Resumé

    Reduced complement-mediated solubilization (CMS) of pre-formed immune complexes (IC) was demonstrated in sera from 11 out of 12 SLE patients. The presence of incompletely solubilized endogeneous IC in SLE sera was indicated by the following findings: (1) When IC positive SLE sera with reduced CMS capacity were mixed with normal donor sera they inhibited the CMS of the latter sera. (2) Resuspended PEG (2.75%) precipitates obtained from SLE sera inhibited the CMS of normal donor sera. (3) Non-solubilized or incompletely complement solubilized IC in SLE sera give a strong response in the PEG-CC assay for IC. The IC activity of SLE sera was clearly reduced in this assay when the endogeneous IC were solubilized prior to testing. In contrast, sera of 14 rheumatoid arthritis (RA) patients exhibited normal CMS. IC which could be further solubilized by complement were not demonstrable although all RA sera were IC positive.

    OriginalsprogEngelsk
    TidsskriftClinical and Experimental Immunology
    Vol/bind54
    Udgave nummer2
    Sider (fra-til)439-47
    ISSN0009-9104
    StatusUdgivet - nov. 1983

    Fingeraftryk

    Serum

    Emneord

    • Adult
    • Aged
    • Antigen-Antibody Complex
    • Arthritis, Rheumatoid
    • Chromatography, Gel
    • Complement Fixation Tests
    • Complement System Proteins
    • Female
    • Humans
    • Immunoglobulin G
    • Lupus Erythematosus, Systemic
    • Male
    • Middle Aged
    • Polyethylene Glycols
    • Precipitin Tests
    • Solubility

    Citer dette

    @article{dcdcfeeae67d41858d7d19b52899360c,
    title = "Reduced complement-mediated immune complex solubilizing capacity and the presence of incompletely solubilized immune complexes in SLE sera",
    abstract = "Reduced complement-mediated solubilization (CMS) of pre-formed immune complexes (IC) was demonstrated in sera from 11 out of 12 SLE patients. The presence of incompletely solubilized endogeneous IC in SLE sera was indicated by the following findings: (1) When IC positive SLE sera with reduced CMS capacity were mixed with normal donor sera they inhibited the CMS of the latter sera. (2) Resuspended PEG (2.75{\%}) precipitates obtained from SLE sera inhibited the CMS of normal donor sera. (3) Non-solubilized or incompletely complement solubilized IC in SLE sera give a strong response in the PEG-CC assay for IC. The IC activity of SLE sera was clearly reduced in this assay when the endogeneous IC were solubilized prior to testing. In contrast, sera of 14 rheumatoid arthritis (RA) patients exhibited normal CMS. IC which could be further solubilized by complement were not demonstrable although all RA sera were IC positive.",
    keywords = "Adult, Aged, Antigen-Antibody Complex, Arthritis, Rheumatoid, Chromatography, Gel, Complement Fixation Tests, Complement System Proteins, Female, Humans, Immunoglobulin G, Lupus Erythematosus, Systemic, Male, Middle Aged, Polyethylene Glycols, Precipitin Tests, Solubility",
    author = "Gunnar Baatrup and I Petersen and Jensenius, {J C} and Svehag, {S E}",
    year = "1983",
    month = "11",
    language = "English",
    volume = "54",
    pages = "439--47",
    journal = "Clinical and Experimental Immunology",
    issn = "0009-9104",
    publisher = "Wiley-Blackwell",
    number = "2",

    }

    Reduced complement-mediated immune complex solubilizing capacity and the presence of incompletely solubilized immune complexes in SLE sera. / Baatrup, Gunnar; Petersen, I; Jensenius, J C; Svehag, S E.

    I: Clinical and Experimental Immunology, Bind 54, Nr. 2, 11.1983, s. 439-47.

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

    TY - JOUR

    T1 - Reduced complement-mediated immune complex solubilizing capacity and the presence of incompletely solubilized immune complexes in SLE sera

    AU - Baatrup, Gunnar

    AU - Petersen, I

    AU - Jensenius, J C

    AU - Svehag, S E

    PY - 1983/11

    Y1 - 1983/11

    N2 - Reduced complement-mediated solubilization (CMS) of pre-formed immune complexes (IC) was demonstrated in sera from 11 out of 12 SLE patients. The presence of incompletely solubilized endogeneous IC in SLE sera was indicated by the following findings: (1) When IC positive SLE sera with reduced CMS capacity were mixed with normal donor sera they inhibited the CMS of the latter sera. (2) Resuspended PEG (2.75%) precipitates obtained from SLE sera inhibited the CMS of normal donor sera. (3) Non-solubilized or incompletely complement solubilized IC in SLE sera give a strong response in the PEG-CC assay for IC. The IC activity of SLE sera was clearly reduced in this assay when the endogeneous IC were solubilized prior to testing. In contrast, sera of 14 rheumatoid arthritis (RA) patients exhibited normal CMS. IC which could be further solubilized by complement were not demonstrable although all RA sera were IC positive.

    AB - Reduced complement-mediated solubilization (CMS) of pre-formed immune complexes (IC) was demonstrated in sera from 11 out of 12 SLE patients. The presence of incompletely solubilized endogeneous IC in SLE sera was indicated by the following findings: (1) When IC positive SLE sera with reduced CMS capacity were mixed with normal donor sera they inhibited the CMS of the latter sera. (2) Resuspended PEG (2.75%) precipitates obtained from SLE sera inhibited the CMS of normal donor sera. (3) Non-solubilized or incompletely complement solubilized IC in SLE sera give a strong response in the PEG-CC assay for IC. The IC activity of SLE sera was clearly reduced in this assay when the endogeneous IC were solubilized prior to testing. In contrast, sera of 14 rheumatoid arthritis (RA) patients exhibited normal CMS. IC which could be further solubilized by complement were not demonstrable although all RA sera were IC positive.

    KW - Adult

    KW - Aged

    KW - Antigen-Antibody Complex

    KW - Arthritis, Rheumatoid

    KW - Chromatography, Gel

    KW - Complement Fixation Tests

    KW - Complement System Proteins

    KW - Female

    KW - Humans

    KW - Immunoglobulin G

    KW - Lupus Erythematosus, Systemic

    KW - Male

    KW - Middle Aged

    KW - Polyethylene Glycols

    KW - Precipitin Tests

    KW - Solubility

    M3 - Journal article

    VL - 54

    SP - 439

    EP - 447

    JO - Clinical and Experimental Immunology

    JF - Clinical and Experimental Immunology

    SN - 0009-9104

    IS - 2

    ER -