TY - JOUR
T1 - Recent advances in practical methods for liver cell biology
T2 - A short overview
AU - Torres, Sandra
AU - Abdullah, Zeinab
AU - Brol, Maximilian J.
AU - Hellerbrand, Claus
AU - Fernandez, Mercedes
AU - Fiorotto, Romina
AU - Klein, Sabine
AU - Königshofer, Philipp
AU - Liedtke, Christian
AU - Lotersztajn, Sophie
AU - Nevzorova, Yulia A.
AU - Schierwagen, Robert
AU - Reiberger, Thomas
AU - Uschner, Frank Erhard
AU - Tacke, Frank
AU - Weiskirchen, Ralf
AU - Trebicka, Jonel
PY - 2020
Y1 - 2020
N2 - Molecular and cellular research modalities for the study of liver pathologies have been tremendously improved over the recent decades. Advanced technologies offer novel opportunities to establish cell isolation techniques with excellent purity, paving the path for 2D and 3D microscopy and high-throughput assays (e.g., bulk or single-cell RNA sequencing). The use of stem cell and organoid research will help to decipher the pathophysiology of liver diseases and the interaction between various parenchymal and non-parenchymal liver cells. Furthermore, sophisticated animal models of liver disease allow for the in vivo assessment of fibrogenesis, portal hypertension and hepatocellular carcinoma (HCC) and for the preclinical testing of therapeutic strategies. The purpose of this review is to portray in detail novel in vitro and in vivo methods for the study of liver cell biology that had been presented at the workshop of the 8th meeting of the European Club for Liver Cell Biology (ECLCB-8) in October of 2018 in Bonn, Germany.
AB - Molecular and cellular research modalities for the study of liver pathologies have been tremendously improved over the recent decades. Advanced technologies offer novel opportunities to establish cell isolation techniques with excellent purity, paving the path for 2D and 3D microscopy and high-throughput assays (e.g., bulk or single-cell RNA sequencing). The use of stem cell and organoid research will help to decipher the pathophysiology of liver diseases and the interaction between various parenchymal and non-parenchymal liver cells. Furthermore, sophisticated animal models of liver disease allow for the in vivo assessment of fibrogenesis, portal hypertension and hepatocellular carcinoma (HCC) and for the preclinical testing of therapeutic strategies. The purpose of this review is to portray in detail novel in vitro and in vivo methods for the study of liver cell biology that had been presented at the workshop of the 8th meeting of the European Club for Liver Cell Biology (ECLCB-8) in October of 2018 in Bonn, Germany.
KW - Fibrogenesis
KW - Hepatic stellate cells
KW - Hepatocellular cancer
KW - In vitro models
KW - Steatosis
U2 - 10.3390/ijms21062027
DO - 10.3390/ijms21062027
M3 - Journal article
C2 - 32188134
AN - SCOPUS:85082072085
VL - 21
JO - International Journal of Molecular Sciences
JF - International Journal of Molecular Sciences
SN - 1661-6596
IS - 6
M1 - 2027
ER -